(Circular) (Six-base) MAPSORT of: pFN21AE0774.seq Check: 2625 from: 1 to: 6040 pFN21AE0774 6040 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2746 3150 3383 1937 Size: 809 404 233 4594 Fragments arranged by size: 4594 809 404 233 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2901 2928 4103 5487 5755 1632 Size: 1269 27 1175 1384 268 1917 Fragments arranged by size: 1917 1384 1269 1175 268 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 4939 5916 1203 Size: 3736 977 1327 Fragments arranged by size: 3736 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 4769 4769 Size: 6040 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AlwNI CAG_nnn'CTG Cuts at: 3026 3783 5355 3026 Size: 757 1572 3711 Fragments arranged by size: 3711 1572 757 ApaI G_GGCC'C Cuts at: 3092 3092 Size: 6040 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 2537 2831 3104 3349 3575 3727 5836 1493 Size: 1044 294 273 245 226 152 2109 1697 Fragments arranged by size: 2109 1697 1044 294 273 245 226 152 AseI AT'TA_AT Cuts at: 160 1032 2459 2667 4594 160 Size: 872 1427 208 1927 1606 Fragments arranged by size: 1927 1606 1427 872 208 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 3365 1870 Size: 73 1422 4545 Fragments arranged by size: 4545 1422 73 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 3361 4816 618 Size: 325 217 627 258 1316 1455 1842 Fragments arranged by size: 1842 1455 1316 627 325 258 217 BanII G_rGCy'C Cuts at: 1391 3092 3125 3359 1391 Size: 1701 33 234 4072 Fragments arranged by size: 4072 1701 234 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2211 928 Size: 971 312 4757 Fragments arranged by size: 4757 971 312 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 3115 1548 Size: 1567 4473 BfrBI ATG'CAT Cuts at: 2456 2456 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4651 136 Size: 107 122 71 631 605 2979 1525 Fragments arranged by size: 2979 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6035 6035 Size: 6040 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 2048 3092 4101 5257 1790 Size: 258 1044 1009 1156 2573 Fragments arranged by size: 2573 1156 1044 1009 258 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3771 4729 442 Size: 416 53 83 399 24 2354 958 1753 Fragments arranged by size: 2354 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3109 4700 1239 Size: 1870 1591 2579 Fragments arranged by size: 2579 1870 1591 Bpu10I CC'TnA_GC Cuts at: 2725 2725 Size: 6040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4165 5030 5328 4165 Size: 865 298 4877 Fragments arranged by size: 4877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2069 4703 882 Size: 617 570 2634 2219 Fragments arranged by size: 2634 2219 617 570 BsaAI yAC'GTr Cuts at: 493 1204 3202 3831 493 Size: 711 1998 629 2702 Fragments arranged by size: 2702 1998 711 629 BsaBI GATnn'nnATC Cuts at: 6034 6034 Size: 6040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3847 4229 4864 275 Size: 53 83 186 936 2314 382 635 1451 Fragments arranged by size: 2314 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4473 4871 5145 5292 5703 4473 Size: 398 274 147 411 4810 Fragments arranged by size: 4810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 1178 Size: 30 165 30 621 30 5164 Fragments arranged by size: 5164 621 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6040 BseYI C'CCAG_C Cuts at: 5243 5739 5243 Size: 496 5544 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5467 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3490 4251 5279 1540 Size: 275 186 1489 761 1028 2301 Fragments arranged by size: 2301 1489 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2726 4101 4186 5257 2726 Size: 1375 85 1071 3509 Fragments arranged by size: 3509 1375 1071 85 BsmI GAATG_Cn' Cuts at: 3551 3644 3551 Size: 93 5947 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2726 3092 3125 3359 4101 4186 Size: 399 258 678 366 33 234 742 85 Cuts at: 4186 5257 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 678 399 366 258 234 85 33 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 3042 3929 5659 3042 Size: 887 1730 3423 Fragments arranged by size: 3423 1730 887 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3395 844 Size: 2551 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3927 1394 Size: 429 2104 3507 Fragments arranged by size: 3507 2104 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4535 4709 65 Size: 1183 3287 174 1396 Fragments arranged by size: 3287 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4684 4871 1700 Size: 110 2874 187 2869 Fragments arranged by size: 2874 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 4100 5112 4100 Size: 1012 5028 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6040 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6040 BstXI CCAn_nnnn'nTGG Cuts at: 2890 3161 2890 Size: 271 5769 BstYI r'GATC_y Cuts at: 1972 2123 2632 3022 3058 3235 3370 3737 4122 Size: 151 509 390 36 177 135 367 385 Cuts at: 4122 4139 5591 5677 5689 6035 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 509 390 385 367 346 177 151 135 86 36 17 12 BstZ17I GTA'TAC Cuts at: 2747 2747 Size: 6040 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5488 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5301 Fragments arranged by size: 5301 683 56 BtsI GCAGTG_nn' Cuts at: 2860 3556 4350 4370 2860 Size: 696 794 20 4530 Fragments arranged by size: 4530 794 696 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6005 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3290 3487 4376 Size: 54 1425 50 275 18 1460 197 889 Cuts at: 4376 6005 8 Size: 1629 43 Fragments arranged by size: 1629 1460 1425 889 275 197 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3487 1537 Size: 275 1675 4090 Fragments arranged by size: 4090 1675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2238 2424 2926 3970 1353 Size: 885 186 502 1044 3423 Fragments arranged by size: 3423 1044 885 502 186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4627 5001 5147 387 Size: 1064 138 3038 374 146 1280 Fragments arranged by size: 3038 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2901 2928 3109 4103 4700 5487 5755 Size: 393 1269 27 181 994 597 787 268 Cuts at: 5755 1239 Size: 1524 Fragments arranged by size: 1524 1269 994 787 597 393 268 181 27 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 5187 1678 Size: 3509 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3060 3092 3107 3139 3304 Size: 32 135 32 1488 32 15 32 165 Cuts at: 3304 3336 4681 4713 4755 4787 1373 Size: 32 1345 32 42 32 2626 Fragments arranged by size: 2626 1488 1345 165 135 42 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 3384 3630 669 Size: 988 281 1446 246 3079 Fragments arranged by size: 3079 1446 988 281 246 HindIII A'AGCT_T Cuts at: 748 2787 748 Size: 2039 4001 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5154 5338 1305 Size: 3849 184 2007 Fragments arranged by size: 3849 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3292 10 Size: 54 1425 343 1460 2758 Fragments arranged by size: 2758 1460 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3159 3998 4357 4516 518 Size: 648 1993 839 359 159 2042 Fragments arranged by size: 2042 1993 839 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2088 3456 4133 5281 5526 1606 Size: 482 1368 677 1148 245 2120 Fragments arranged by size: 2120 1368 1148 677 482 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5488 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NsiI A_TGCA'T Cuts at: 2458 2458 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 2690 3398 4943 1132 Size: 97 1461 708 1545 2229 Fragments arranged by size: 2229 1545 1461 708 97 PacI TTA_AT'TAA Cuts at: 2667 2667 Size: 6040 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6040 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6040 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3183 3610 3183 Size: 427 5613 PspOMI G'GGCC_C Cuts at: 3088 3088 Size: 6040 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2794 2826 2794 Size: 32 6008 PstI C_TGCA'G Cuts at: 830 2386 3217 3392 830 Size: 1556 831 175 3478 Fragments arranged by size: 3478 1556 831 175 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SapI GCTCTTCn'nnn_ Cuts at: 2238 2424 2238 Size: 186 5854 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 2382 3213 3245 3388 4523 5204 5395 Size: 219 1337 831 32 143 1135 681 191 Cuts at: 5395 826 Size: 1471 Fragments arranged by size: 1471 1337 1135 831 681 219 191 143 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3367 1872 Size: 1495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4144 5045 5307 820 Size: 197 926 2201 901 262 1553 Fragments arranged by size: 2201 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3202 3831 493 Size: 2709 629 2702 Fragments arranged by size: 2709 2702 629 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6040 StyI C'CwwG_G Cuts at: 513 1065 2418 2883 3479 513 Size: 552 1353 465 596 3074 Fragments arranged by size: 3074 1353 596 552 465 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4067 4084 4237 4422 4867 325 Size: 1255 2487 17 153 185 445 1498 Fragments arranged by size: 2487 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4286 5998 96 Size: 274 80 33 51 1443 2309 1712 138 Fragments arranged by size: 2309 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2024 2056 6000 6032 2024 Size: 32 3944 32 2032 Fragments arranged by size: 3944 2032 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3365 1870 Size: 1495 4545 XmnI GAAnn'nnTTC Cuts at: 3108 4169 3108 Size: 1061 4979 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PmlI PpuMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI