Restriction map for
pFN21AE0736
(Circular) (Six-base) MAPSORT of: pFN21AE0736.seq Check: 929 from: 1 to: 5974
pFN21AE0736 5974 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2361 3154 4037 5421 5689 1632
Size: 729 793 883 1384 268 1917
Fragments arranged by size:
1917 1384 883 793 729 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 3237 4873 5850 1203
Size: 2034 1636 977 1327
Fragments arranged by size:
2034 1636 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3216 1184
Size: 2032 3942
AlwNI CAG_nnn'CTG
Cuts at: 3717 5289 3717
Size: 1572 4402
ApaI G_GGCC'C
Cuts at: 2551 2551
Size: 5974
ApaLI G'TGCA_C
Cuts at: 3191 4031 5187 3191
Size: 840 1156 3978
Fragments arranged by size:
3978 1156 840
ApoI r'AATT_y
Cuts at: 1493 3283 3509 3661 5770 1493
Size: 1790 226 152 2109 1697
Fragments arranged by size:
2109 1790 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2559 3299 1870
Size: 73 616 740 4545
Fragments arranged by size:
4545 740 616 73
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2190 2386 2424 2462 2551 2778 2916 3293
Size: 799 196 38 38 89 227 138 377
Cuts at: 3293 1391
Size: 4072
Fragments arranged by size:
4072 799 377 227 196 138 89 38
38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 928
Size: 971 429 4574
Fragments arranged by size:
4574 971 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4165 4199 1903
Size: 34 50 34 2144 34 3678
Fragments arranged by size:
3678 2144 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 2365 3391 2365
Size: 1026 4948
Bme1580I G_kGCm'C
Cuts at: 1790 2551 3195 4035 5191 1790
Size: 761 644 840 1156 2573
Fragments arranged by size:
2573 1156 840 761 644
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2794 3705 4663
Size: 416 53 83 399 24 1377 911 958
Cuts at: 4663 442
Size: 1753
Fragments arranged by size:
1753 1377 958 911 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2804 4634 1239
Size: 839 726 1830 2579
Fragments arranged by size:
2579 1830 839 726
Bpu10I CC'TnA_GC
Cuts at: 2772 2903 2772
Size: 131 5843
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3071 4099 4964 5262 3071
Size: 1028 865 298 3783
Fragments arranged by size:
3783 1028 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2082 3050 4637 882
Size: 617 583 968 1587 2219
Fragments arranged by size:
2219 1587 968 617 583
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3000 3781 4163 4798
Size: 53 83 186 936 1467 781 382 635
Cuts at: 4798 275
Size: 1451
Fragments arranged by size:
1467 1451 936 781 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2048 2750 2812 3264 4407 4805 5079 5226 5637
Size: 702 62 452 1143 398 274 147 411
Cuts at: 5637 2048
Size: 2385
Fragments arranged by size:
2385 1143 702 452 411 398 274 147
62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2010 2040 2140 2170 1178
Size: 30 165 30 607 30 100 30 4982
Fragments arranged by size:
4982 607 165 100 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2531 2615 2858 1186
Size: 858 487 84 243 4302
Fragments arranged by size:
4302 858 487 243 84
BseYI C'CCAG_C
Cuts at: 2127 2777 2915 3148 5177 5673 2127
Size: 650 138 233 2029 496 2428
Fragments arranged by size:
2428 2029 650 496 233 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3195 4035 4120 5191 3195
Size: 840 85 1071 3978
Fragments arranged by size:
3978 1071 840 85
BsmI GAATG_Cn'
Cuts at: 3485 3578 3485
Size: 93 5881
BsmBI CGTCTCn'nnnn_
Cuts at: 2160 2160
Size: 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2386 2424 2462 2551 2778 2916
Size: 399 400 196 38 38 89 227 138
Cuts at: 2916 3195 3293 4035 4120 5191 1391
Size: 279 98 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 279 227 196
138 98 89 85 38 38
BspEI T'CCGG_A
Cuts at: 3264 5637 3264
Size: 2373 3601
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3018 3329 844
Size: 2174 311 3489
Fragments arranged by size:
3489 2174 311
BsrBI CCG'CTC
Cuts at: 1394 1823 2635 3861 1394
Size: 429 812 1226 3507
Fragments arranged by size:
3507 1226 812 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstXI CCAn_nnnn'nTGG
Cuts at: 3040 3040
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2451 2941 3304 3671 4056 4073 5525 5611
Size: 479 490 363 367 385 17 1452 86
Cuts at: 5611 5623 5969 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 490 479 385 367 363 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2598 2598
Size: 5974
BtgI C'CryG_G
Cuts at: 513 1065 2211 513
Size: 552 1146 4276
Fragments arranged by size:
4276 1146 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2170 2948 3490 4284 4304 2170
Size: 778 542 794 20 3840
Fragments arranged by size:
3840 794 778 542 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2666 4981 809
Size: 507 1350 2315 1802
Fragments arranged by size:
2315 1802 1350 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2445 2836 3258
Size: 54 1425 50 275 18 615 391 422
Cuts at: 3258 3421 4310 5939 8
Size: 163 889 1629 43
Fragments arranged by size:
1629 1425 889 615 422 391 275 163
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2813 3904 1353
Size: 1460 1091 3423
Fragments arranged by size:
3423 1460 1091
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2113 2266 4561 4935 5081 387
Size: 1064 138 524 153 2295 374 146 1280
Fragments arranged by size:
2295 1280 1064 524 374 153 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2361 2804 3154 4037 4634 5421
Size: 393 446 283 443 350 883 597 787
Cuts at: 5421 5689 1239
Size: 268 1524
Fragments arranged by size:
1524 883 787 597 446 443 393 350
283 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2576 2576
Size: 5974
EcoO109I rG'GnC_Cy
Cuts at: 2021 2395 2547 2548 2592 2021
Size: 374 152 1 44 5403
Fragments arranged by size:
5403 374 152 44 1
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 5974
FspI TGC'GCA
Cuts at: 2270 3047 4480 2270
Size: 777 1433 3764
Fragments arranged by size:
3764 1433 777
HaeII r_GCGC'y
Cuts at: 1678 2037 2799 5121 1678
Size: 359 762 2322 2531
Fragments arranged by size:
2531 2322 762 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2461 2493 4615 4647 4689
Size: 32 135 32 889 32 2122 32 42
Cuts at: 4689 4721 1373
Size: 32 2626
Fragments arranged by size:
2626 2122 889 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2659 3318 3564 669
Size: 988 281 721 659 246 3079
Fragments arranged by size:
3079 988 721 659 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3260 10
Size: 54 1425 343 1428 2724
Fragments arranged by size:
2724 1428 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3110 3141 3198 3932 4291 4450 518
Size: 648 1944 31 57 734 359 159 2042
Fragments arranged by size:
2042 1944 734 648 359 159 57 31
MspA1I CmG'CkG
Cuts at: 1606 2707 3390 4067 5215 5460 1606
Size: 1101 683 677 1148 245 2120
Fragments arranged by size:
2120 1148 1101 683 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5422
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 3241 3332 4877 1132
Size: 97 2012 91 1545 2229
Fragments arranged by size:
2229 2012 1545 97 91
PasI CC'CwG_GG
Cuts at: 1348 2616 2926 1348
Size: 1268 310 4396
Fragments arranged by size:
4396 1268 310
PciI A'CATG_T
Cuts at: 3237 4873 3237
Size: 1636 4338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5974
PfoI T'CCnGG_A
Cuts at: 2138 2138
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2021 2395 2021
Size: 374 5600
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspOMI G'GGCC_C
Cuts at: 2547 2547
Size: 5974
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2104 2136 2104
Size: 32 5942
PstI C_TGCA'G
Cuts at: 830 2878 3326 830
Size: 2048 448 3478
Fragments arranged by size:
3478 2048 448
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2707 2707
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SapI GCTCTTCn'nnn_
Cuts at: 2813 2813
Size: 5974
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SexAI A'CCwGG_T
Cuts at: 2964 3039 2964
Size: 75 5899
SfcI C'TryA_G
Cuts at: 826 1045 2041 2874 3322 4457 5138 5329 826
Size: 219 996 833 448 1135 681 191 1471
Fragments arranged by size:
1471 1135 996 833 681 448 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 2561 3301 1872
Size: 689 740 4545
Fragments arranged by size:
4545 740 689
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3086 4078 4979 5241 820
Size: 197 926 1143 992 901 262 1553
Fragments arranged by size:
1553 1143 992 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2106 2463 2684 3129 3413 513
Size: 552 1041 357 221 445 284 3074
Fragments arranged by size:
3074 1041 552 445 357 284 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4001 4018 4171 4356 4801 325
Size: 1255 2421 17 153 185 445 1498
Fragments arranged by size:
2421 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2255 4220 5932
Size: 274 80 33 51 1443 278 1965 1712
Cuts at: 5932 96
Size: 138
Fragments arranged by size:
1965 1712 1443 278 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2010 2042 2140 2172 5934 5966 2010
Size: 32 98 32 3762 32 2018
Fragments arranged by size:
3762 2018 98 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2923 2923
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 2559 3299 1870
Size: 689 740 4545
Fragments arranged by size:
4545 740 689
XmnI GAAnn'nnTTC
Cuts at: 3071 4103 3071
Size: 1032 4942
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI