Restriction map for
pFN21AE0768
(Circular) (Six-base) MAPSORT of: pFN21AE0768.seq Check: 3542 from: 1 to: 6019
pFN21AE0768 6019 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2955 3267 2955
Size: 312 5707
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3829 278
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3362 1937
Size: 1425 4594
Acc65I G'GTAC_C
Cuts at: 3340 3340
Size: 6019
AclI AA'CG_TT
Cuts at: 1563 4146 4519 1563
Size: 2583 373 3063
Fragments arranged by size:
3063 2583 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2204 2661 2717 4082 5466 5734 1632
Size: 572 457 56 1365 1384 268 1917
Fragments arranged by size:
1917 1384 1365 572 457 268 56
AfeI AGC'GCT
Cuts at: 2070 2070
Size: 6019
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5822
AflIII A'CryG_T
Cuts at: 1203 3120 4918 5895 1203
Size: 1917 1798 977 1327
Fragments arranged by size:
1917 1798 1327 977
AgeI A'CCGG_T
Cuts at: 4850 4850
Size: 6019
AhdI GACnn_n'nnGTC
Cuts at: 4748 4748
Size: 6019
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6019
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6019
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2496 2528 2496
Size: 32 5987
AlwNI CAG_nnn'CTG
Cuts at: 2326 3762 5334 2326
Size: 1436 1572 3011
Fragments arranged by size:
3011 1572 1436
ApaI G_GGCC'C
Cuts at: 2724 2724
Size: 6019
ApaLI G'TGCA_C
Cuts at: 3124 4076 5232 3124
Size: 952 1156 3911
Fragments arranged by size:
3911 1156 952
ApoI r'AATT_y
Cuts at: 1493 2986 3328 3554 3706 5815 1493
Size: 1493 342 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 342 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4573 160
Size: 872 3541 1606
Fragments arranged by size:
3541 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
AvaI C'yCGr_G
Cuts at: 1870 1943 3344 1870
Size: 73 1401 4545
Fragments arranged by size:
4545 1401 73
BamHI G'GATC_C
Cuts at: 3349 3716 3349
Size: 367 5652
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2931 3156 3255 3340 4795
Size: 325 217 627 1144 225 99 85 1455
Cuts at: 4795 618
Size: 1842
Fragments arranged by size:
1842 1455 1144 627 325 225 217 99
85
BanII G_rGCy'C
Cuts at: 1391 2121 2724 3074 3338 1391
Size: 730 603 350 264 4072
Fragments arranged by size:
4072 730 603 350 264
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2906 2920 928
Size: 971 1007 14 4027
Fragments arranged by size:
4027 1007 971 14
BbvCI CC'TCA_GC
Cuts at: 2013 2013
Size: 6019
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4210 4244 1903
Size: 34 50 34 2189 34 3678
Fragments arranged by size:
3678 2189 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2584 3911 5121 2584
Size: 1327 1210 3482
Fragments arranged by size:
3482 1327 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6019
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4630 136
Size: 107 122 71 631 605 2958 1525
Fragments arranged by size:
2958 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6014 6014
Size: 6019
BlpI GC'TnA_GC
Cuts at: 3436 3436
Size: 6019
Bme1580I G_kGCm'C
Cuts at: 1790 2724 3100 3128 3212 4080 5236 1790
Size: 934 376 28 84 868 1156 2573
Fragments arranged by size:
2573 1156 934 868 376 84 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6019
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2293 2800 2874
Size: 416 53 83 399 24 876 507 74
Cuts at: 2874 3198 3750 4708 442
Size: 324 552 958 1753
Fragments arranged by size:
1753 958 876 552 507 416 399 324
83 74 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6019
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2729 2729
Size: 6019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2298 2686 4679 1239
Size: 959 100 388 1993 2579
Fragments arranged by size:
2579 1993 959 388 100
Bpu10I CC'TnA_GC
Cuts at: 2013 2013
Size: 6019
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2873 4144 5009 5307 2873
Size: 1271 865 298 3585
Fragments arranged by size:
3585 1271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2866 4682 882
Size: 617 1367 1816 2219
Fragments arranged by size:
2219 1816 1367 617
BsaAI yAC'GTr
Cuts at: 493 1204 3121 3810 493
Size: 711 1917 689 2702
Fragments arranged by size:
2702 1917 711 689
BsaBI GATnn'nnATC
Cuts at: 2086 6013 2086
Size: 3927 2092
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3826 4208 4843 275
Size: 53 83 186 936 2293 382 635 1451
Fragments arranged by size:
2293 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4452 4850 5124 5271 5682 4452
Size: 398 274 147 411 4789
Fragments arranged by size:
4789 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2198 2201 2228 2400
Size: 30 165 30 768 27 3 27 172
Cuts at: 2400 2430 1178
Size: 30 4767
Fragments arranged by size:
4767 768 172 165 30 30 30 27
27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2381 2511 2549 1186
Size: 1033 162 130 38 4656
Fragments arranged by size:
4656 1033 162 130 38
BseYI C'CCAG_C
Cuts at: 2071 2679 2693 3294 5222 5718 2071
Size: 608 14 601 1928 496 2372
Fragments arranged by size:
2372 1928 608 601 496 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2283 1163
Size: 573 547 4899
Fragments arranged by size:
4899 573 547
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3469 4230 5258 1540
Size: 275 186 1468 761 1028 2301
Fragments arranged by size:
2301 1468 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2184 2972 3128 4080 4165 5236 2184
Size: 788 156 952 85 1071 2967
Fragments arranged by size:
2967 1071 952 788 156 85
BsmI GAATG_Cn'
Cuts at: 2715 3530 3623 2715
Size: 815 93 5111
Fragments arranged by size:
5111 815 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2184 2724 2972 3074 3100 3128
Size: 399 331 63 540 248 102 26 28
Cuts at: 3128 3212 3338 4080 4165 5236 1391
Size: 84 126 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 540 399 331 248 126
102 85 84 63 28 26
BspEI T'CCGG_A
Cuts at: 5682 5682
Size: 6019
BspHI T'CATG_A
Cuts at: 3908 5638 3908
Size: 1730 4289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2763 2955 3267 3374 844
Size: 1919 192 312 107 3489
Fragments arranged by size:
3489 1919 312 192 107
BsrBI CCG'CTC
Cuts at: 1394 1823 2149 3906 1394
Size: 429 326 1757 3507
Fragments arranged by size:
3507 1757 429 326
BsrDI GCAATG_nn'
Cuts at: 65 1248 4514 4688 65
Size: 1183 3266 174 1396
Fragments arranged by size:
3266 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4663 4850 1700
Size: 110 2853 187 2869
Fragments arranged by size:
2869 2853 187 110
BsrGI T'GTAC_A
Cuts at: 96 2098 96
Size: 2002 4017
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6019
BssSI C'ACGA_G
Cuts at: 4079 5091 4079
Size: 1012 5007
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2306 3045 794
Size: 1512 739 3768
Fragments arranged by size:
3768 1512 739
BstBI TT'CG_AA
Cuts at: 4823 4823
Size: 6019
BstEII G'GTnAC_C
Cuts at: 1184 2329 1184
Size: 1145 4874
BstXI CCAn_nnnn'nTGG
Cuts at: 3062 3062
Size: 6019
BstYI r'GATC_y
Cuts at: 1972 3349 3716 4101 4118 5570 5656 5668 6014
Size: 1377 367 385 17 1452 86 12 346
Cuts at: 6014 1972
Size: 1977
Fragments arranged by size:
1977 1452 1377 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2153 513
Size: 552 1088 4379
Fragments arranged by size:
4379 1088 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5280
Fragments arranged by size:
5280 683 56
BtsI GCAGTG_nn'
Cuts at: 3535 4329 4349 3535
Size: 794 20 5205
Fragments arranged by size:
5205 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5984
DraI TTT'AAA
Cuts at: 3323 3323
Size: 6019
DraIII CAC_nnn'GTG
Cuts at: 3166 3166
Size: 6019
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5026 809
Size: 507 3710 1802
Fragments arranged by size:
3710 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2150 3009 3148
Size: 54 1425 50 275 18 320 859 139
Cuts at: 3148 3232 3466 4355 5984 8
Size: 84 234 889 1629 43
Fragments arranged by size:
1629 1425 889 859 320 275 234 139
84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3466 1537
Size: 275 1654 4090
Fragments arranged by size:
4090 1654 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 2196 3134 3218 3949 1353
Size: 786 57 938 84 731 3423
Fragments arranged by size:
3423 938 786 731 84 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2389 4606 4980 5126 387
Size: 1064 138 800 2217 374 146 1280
Fragments arranged by size:
2217 1280 1064 800 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2204 2298 2661 2686 2717 4082
Size: 393 566 6 94 363 25 31 1365
Cuts at: 4082 4679 5466 5734 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1365 787 597 566 393 363 268
94 31 25 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2201 2201
Size: 6019
EcoO109I rG'GnC_Cy
Cuts at: 2171 2323 2512 2720 2721 2171
Size: 152 189 208 1 5469
Fragments arranged by size:
5469 208 189 152 1
EcoRI G'AATT_C
Cuts at: 3328 3706 3328
Size: 378 5641
FspI TGC'GCA
Cuts at: 4525 4525
Size: 6019
HaeII r_GCGC'y
Cuts at: 1678 2072 5166 1678
Size: 394 3094 2531
Fragments arranged by size:
3094 2531 394
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2198 2230 2400
Size: 32 135 32 434 32 160 32 170
Cuts at: 2400 2432 2732 2764 2907 2939 4660 4692 4734
Size: 32 300 32 143 32 1721 32 42
Cuts at: 4734 4766 1373
Size: 32 2626
Fragments arranged by size:
2626 1721 434 300 170 160 143 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3363 3609 669
Size: 988 281 1425 246 3079
Fragments arranged by size:
3079 1425 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6019
HpaI GTT'AAC
Cuts at: 3609 3609
Size: 6019
KpnI G_GTAC'C
Cuts at: 3344 3344
Size: 6019
MfeI C'AATT_G
Cuts at: 3618 3618
Size: 6019
MluI A'CGCG_T
Cuts at: 5895 5895
Size: 6019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3043 5133 5317 1305
Size: 1738 2090 184 2007
Fragments arranged by size:
2090 2007 1738 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3011 10
Size: 54 1425 343 1179 3018
Fragments arranged by size:
3018 1425 1179 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2771 2949 3977 4336 4495 518
Size: 648 1605 178 1028 359 159 2042
Fragments arranged by size:
2042 1605 1028 648 359 178 159
MspA1I CmG'CkG
Cuts at: 1606 2104 2297 2883 3294 3435 4112 5260 5505
Size: 498 193 586 411 141 677 1148 245
Cuts at: 5505 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 586 498 411 245 193
141
NcoI C'CATG_G
Cuts at: 513 1065 2153 513
Size: 552 1088 4379
Fragments arranged by size:
4379 1088 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6019
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6019
NotI GC'GGCC_GC
Cuts at: 3466 3466
Size: 6019
NspI r_CATG'y
Cuts at: 1132 1229 2479 2802 3032 3377 4922 1132
Size: 97 1250 323 230 345 1545 2229
Fragments arranged by size:
2229 1545 1250 345 323 230 97
PasI CC'CwG_GG
Cuts at: 1348 2244 1348
Size: 896 5123
PciI A'CATG_T
Cuts at: 4918 4918
Size: 6019
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6019
PmeI GTTT'AAAC
Cuts at: 3323 3323
Size: 6019
PmlI CAC'GTG
Cuts at: 3121 3121
Size: 6019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4088 4120 5601 5633 4088
Size: 32 1481 32 4474
Fragments arranged by size:
4474 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2171 2512 2171
Size: 341 5678
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6019
PsiI TTA'TAA
Cuts at: 3589 3589
Size: 6019
PspOMI G'GGCC_C
Cuts at: 2720 2720
Size: 6019
PstI C_TGCA'G
Cuts at: 830 2041 3371 830
Size: 1211 1330 3478
Fragments arranged by size:
3478 1330 1211
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6019
PvuII CAG'CTG
Cuts at: 2104 2297 2883 3294 2104
Size: 193 586 411 4829
Fragments arranged by size:
4829 586 411 193
SalI G'TCGA_C
Cuts at: 1936 3361 1936
Size: 1425 4594
SanDI GG'GwC_CC
Cuts at: 2512 2512
Size: 6019
SapI GCTCTTCn'nnn_
Cuts at: 2139 3134 3218 2139
Size: 995 84 4940
Fragments arranged by size:
4940 995 84
SbfI CC_TGCA'GG
Cuts at: 3371 3371
Size: 6019
ScaI AGT'ACT
Cuts at: 4267 5979 4267
Size: 1712 4307
SfcI C'TryA_G
Cuts at: 826 1045 2037 2878 3367 4502 5183 5374 826
Size: 219 992 841 489 1135 681 191 1471
Fragments arranged by size:
1471 1135 992 841 681 489 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6019
SmaI CCC'GGG
Cuts at: 1872 3346 1872
Size: 1474 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2888 4123 5024 5286 820
Size: 197 926 945 1235 901 262 1553
Fragments arranged by size:
1553 1235 945 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3810 493
Size: 3317 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6019
SphI G_CATG'C
Cuts at: 1132 3032 3377 1132
Size: 1900 345 3774
Fragments arranged by size:
3774 1900 345
SspI AAT'ATT
Cuts at: 5 52 3943 5
Size: 47 3891 2081
Fragments arranged by size:
3891 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6019
StyI C'CwwG_G
Cuts at: 513 1065 2153 3458 513
Size: 552 1088 1305 3074
Fragments arranged by size:
3074 1305 1088 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3281 4046 4063 4216 4401 4846 325
Size: 1255 1701 765 17 153 185 445 1498
Fragments arranged by size:
1701 1498 1255 765 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2098 4265 5977
Size: 274 80 33 51 1443 121 2167 1712
Cuts at: 5977 96
Size: 138
Fragments arranged by size:
2167 1712 1443 274 138 121 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2171 2203 5979 6011 2171
Size: 32 3776 32 2179
Fragments arranged by size:
3776 2179 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3279 1316
Size: 1963 4056
XbaI T'CTAG_A
Cuts at: 3355 3355
Size: 6019
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6019
XmaI C'CCGG_G
Cuts at: 1870 3344 1870
Size: 1474 4545
XmnI GAAnn'nnTTC
Cuts at: 3200 4148 3200
Size: 948 5071
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3827 276
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI
RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI
XcmI