################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO109I EcoO65I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 1129 CGCG CG^CG AfaI 821 GTAC GT^AC AflIII 443 ACATGT A^C[GA][CT]GT Alw44I 787 GTGCAC G^TGCAC ApaLI 787 GTGCAC G^TGCAC BalI 879 TGGCCA TGG^CCA BanI 1192 GGTGCC G^G[CT][GA]CC BbsI 1507 GTCTTC GAAGAC(2/6) BbvCI 409 GCTGAGG CCTCAGC(-5/-2) BglII 1362 AGATCT A^GATCT BpuAI 1507 GTCTTC GAAGAC(2/6) BsgI 851 GTGCAG GTGCAG(16/14) BsmI 472 GAATGC GAATGC(1/-1) BspHI 176 TCATGA T^CATGA BspLU11I 443 ACATGT A^CATGT BsrFI 1025 ACCGGC [GA]^CCGG[CT] BstUI 1129 CGCG CG^CG BstYI 1362 AGATCT [GA]^GATC[CT] CfoI 963 GCGC GCG^C Cfr10I 1025 ACCGGC [GA]^CCGG[CT] DraIII 1076 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 879 TGGCCA [CT]^GGCC[GA] EcoRI 9 GAATTC G^AATTC EcoRV 652 GATATC GAT^ATC FauI 491 CCCGC CCCGC(4/6) HhaI 963 GCGC GCG^C HinP1I 963 GCGC G^CGC HincII 991 GTTGAC GT[CT]^[GA]AC HindII 991 GTTGAC GT[CT]^[GA]AC MflI 1362 AGATCT [GA]^GATC[CT] MluNI 879 TGGCCA TGG^CCA MscI 879 TGGCCA TGG^CCA MvnI 1129 CGCG CG^CG NheI 70 GCTAGC G^CTAGC RcaI 176 TCATGA T^CATGA RsaI 821 GTAC GT^AC SmaI 1054 CCCGGG CCC^GGG SphI 1340 GCATGC GCATG^C StuI 1448 AGGCCT AGG^CCT TaqI 405 TCGA T^CGA TfiI 79 GAATC G^A[AT]TC TthHB8I 405 TCGA T^CGA XbaI 189 TCTAGA T^CTAGA XhoII 1362 AGATCT [GA]^GATC[CT] XmaCI 1054 CCCGGG C^CCGGG XmaI 1054 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 364 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1181 CAGGGGCTG AocI 14 CCTCAGG CC^T[ACGT]AGG 1444 CCTGAGG AvaI 1019 CTCGGG C^[CT]CG[GA]G 1054 CCCGGG BsoBI 1019 CTCGGG C^[CT]CG[GA]G 1054 CCCGGG BstXI 218 CCATTTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1465 CCATCCCTCTGG Bsu36I 14 CCTCAGG CC^T[ACGT]AGG 1444 CCTGAGG Eco81I 14 CCTCAGG CC^T[ACGT]AGG 1444 CCTGAGG EcoNI 306 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1440 CCTTCCTGAGG EcoT14I 104 CCATGG C^C[AT][AT]GG 387 CCATGG HindIII 762 AAGCTT A^AGCTT 1549 AAGCTT MseI 719 TTAA T^TAA 1592 TTAA MslI 219 CATTTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1038 CACCCTGGTG NcoI 104 CCATGG C^CATGG 387 CCATGG PstI 337 CTGCAG CTGCA^G 604 CTGCAG SfcI 337 CTGCAG C^T[GA][CT]AG 604 CTGCAG SmlI 24 CTCAAG C^T[CT][GA]AG 167 CTCAAG StyI 104 CCATGG C^C[AT][AT]GG 387 CCATGG Tru9I 719 TTAA T^TAA 1592 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PvuII SacI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI Van91I XcmI