(Circular) (Six-base) MAPSORT of: pFN21AE4135.seq Check: 9910 from: 1 to: 6334 pFN21AE4135 6334 bp With 182 enzymes: SgfI * March 28, 2012 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3677 1937 Size: 1740 4594 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 2507 3408 3555 4397 5781 6049 1632 Size: 701 174 901 147 842 1384 268 1917 Fragments arranged by size: 1917 1384 901 842 701 268 174 147 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 2448 5233 6210 1203 Size: 1245 2785 977 1327 Fragments arranged by size: 2785 1327 1245 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 3047 1166 Size: 1881 4453 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3113 1184 Size: 1929 4405 AlwNI CAG_nnn'CTG Cuts at: 2374 3191 4077 5649 2374 Size: 817 886 1572 3059 Fragments arranged by size: 3059 1572 886 817 ApaLI G'TGCA_C Cuts at: 2792 4391 5547 2792 Size: 1599 1156 3579 Fragments arranged by size: 3579 1599 1156 ApoI r'AATT_y Cuts at: 1493 2014 2430 2624 3643 3869 4021 6130 1493 Size: 521 416 194 1019 226 152 2109 1697 Fragments arranged by size: 2109 1697 1019 521 416 226 194 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 3024 3059 3659 1870 Size: 73 1081 35 600 4545 Fragments arranged by size: 4545 1081 600 73 35 BamHI G'GATC_C Cuts at: 3664 4031 3664 Size: 367 5967 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3197 3655 5110 618 Size: 325 217 627 1410 458 1455 1842 Fragments arranged by size: 1842 1455 1410 627 458 325 217 BanII G_rGCy'C Cuts at: 1391 2208 2565 3464 3653 1391 Size: 817 357 899 189 4072 Fragments arranged by size: 4072 899 817 357 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3505 928 Size: 971 1606 3757 Fragments arranged by size: 3757 1606 971 BbvCI CC'TCA_GC Cuts at: 2415 2415 Size: 6334 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4525 4559 1903 Size: 34 2588 34 3678 Fragments arranged by size: 3678 2588 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4226 5436 4226 Size: 1210 5124 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4945 136 Size: 107 122 71 631 605 3273 1525 Fragments arranged by size: 3273 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3367 6329 3367 Size: 2962 3372 BlpI GC'TnA_GC Cuts at: 3751 3751 Size: 6334 Bme1580I G_kGCm'C Cuts at: 1790 2796 3060 4395 5551 1790 Size: 1006 264 1335 1156 2573 Fragments arranged by size: 2573 1335 1156 1006 264 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3010 3092 4065 Size: 416 53 83 399 24 1593 82 973 Cuts at: 4065 5023 442 Size: 958 1753 Fragments arranged by size: 1753 1593 973 958 416 399 83 82 53 24 BmtI G_CTAG'C Cuts at: 1056 2079 1056 Size: 1023 5311 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2497 2497 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2121 3020 3478 4994 1239 Size: 882 899 458 1516 2579 Fragments arranged by size: 2579 1516 899 882 458 Bpu10I CC'TnA_GC Cuts at: 2415 2559 2415 Size: 144 6190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2014 2157 4459 5324 5622 2014 Size: 143 2302 865 298 2726 Fragments arranged by size: 2726 2302 865 298 143 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4997 882 Size: 617 3498 2219 Fragments arranged by size: 3498 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4125 493 Size: 711 2921 2702 Fragments arranged by size: 2921 2702 711 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4141 4523 5158 275 Size: 53 83 186 936 2608 382 635 1451 Fragments arranged by size: 2608 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4767 5165 5439 5586 5997 4767 Size: 398 274 147 411 5104 Fragments arranged by size: 5104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6109 Fragments arranged by size: 6109 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2213 2216 2432 3251 1186 Size: 922 105 3 216 819 4269 Fragments arranged by size: 4269 922 819 216 105 3 BseYI C'CCAG_C Cuts at: 2604 5537 6033 2604 Size: 2933 496 2905 Fragments arranged by size: 2933 2905 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2877 1163 Size: 573 1141 4620 Fragments arranged by size: 4620 1141 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3784 4545 5573 1540 Size: 275 186 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2208 2565 2796 3016 3464 4395 4480 5551 2208 Size: 357 231 220 448 931 85 1071 2991 Fragments arranged by size: 2991 1071 931 448 357 231 220 85 BsmI GAATG_Cn' Cuts at: 2483 3845 3938 2483 Size: 1362 93 4879 Fragments arranged by size: 4879 1362 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2565 2796 3016 3060 3464 3653 Size: 399 418 357 231 220 44 404 189 Cuts at: 3653 4395 4480 5551 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 418 404 399 357 231 220 189 85 44 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 2181 4223 5953 2181 Size: 2042 1730 2562 Fragments arranged by size: 2562 2042 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3689 844 Size: 2845 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4221 1394 Size: 429 2398 3507 Fragments arranged by size: 3507 2398 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4829 5003 65 Size: 1183 3581 174 1396 Fragments arranged by size: 3581 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3030 4978 5165 1700 Size: 110 1220 1948 187 2869 Fragments arranged by size: 2869 1948 1220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6334 BssSI C'ACGA_G Cuts at: 4394 5406 4394 Size: 1012 5322 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6334 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstXI CCAn_nnnn'nTGG Cuts at: 2230 3477 2230 Size: 1247 5087 BstYI r'GATC_y Cuts at: 1972 3367 3664 4031 4416 4433 5885 5971 5983 Size: 1395 297 367 385 17 1452 86 12 Cuts at: 5983 6329 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1395 385 367 346 297 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2020 3450 2020 Size: 1430 4904 BtgI C'CryG_G Cuts at: 513 1065 2004 2109 2392 2963 513 Size: 552 939 105 283 571 3884 Fragments arranged by size: 3884 939 571 552 283 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5595 Fragments arranged by size: 5595 683 56 BtsI GCAGTG_nn' Cuts at: 2910 3095 3850 4644 4664 2910 Size: 185 755 794 20 4580 Fragments arranged by size: 4580 794 755 185 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2911 2946 529 Size: 35 2347 35 3917 Fragments arranged by size: 3917 2347 35 35 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DraIII CAC_nnn'GTG Cuts at: 3086 3086 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5341 809 Size: 507 4025 1802 Fragments arranged by size: 4025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2884 3630 3781 Size: 54 1425 50 275 18 1054 746 151 Cuts at: 3781 4670 6299 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1054 889 746 275 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3781 1537 Size: 275 1969 4090 Fragments arranged by size: 4090 1969 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4264 1353 Size: 2911 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4921 5295 5441 387 Size: 1064 138 3332 374 146 1280 Fragments arranged by size: 3332 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2206 2563 3462 2206 Size: 357 899 5078 Fragments arranged by size: 5078 899 357 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 2333 2507 3020 3408 3478 3555 Size: 393 489 212 174 513 388 70 77 Cuts at: 3555 4397 4994 5781 6049 1239 Size: 842 597 787 268 1524 Fragments arranged by size: 1524 842 787 597 513 489 393 388 268 212 174 77 70 EcoNI CCTnn'n_nnAGG Cuts at: 2315 3449 2315 Size: 1134 5200 EcoRI G'AATT_C Cuts at: 2014 3643 4021 2014 Size: 1629 378 4327 Fragments arranged by size: 4327 1629 378 EcoRV GAT'ATC Cuts at: 2659 2659 Size: 6334 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2760 2760 Size: 6334 FspI TGC'GCA Cuts at: 4840 4840 Size: 6334 HaeII r_GCGC'y Cuts at: 1678 5481 1678 Size: 3803 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2648 2680 2943 2975 3095 Size: 32 135 32 1076 32 263 32 120 Cuts at: 3095 3127 4975 5007 5049 5081 1373 Size: 32 1848 32 42 32 2626 Fragments arranged by size: 2626 1848 1076 263 135 120 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2998 3678 3924 669 Size: 988 281 1060 680 246 3079 Fragments arranged by size: 3079 1060 988 680 281 246 HindIII A'AGCT_T Cuts at: 748 2767 3554 748 Size: 2019 787 3528 Fragments arranged by size: 3528 2019 787 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2331 3138 5448 5632 1305 Size: 1026 807 2310 184 2007 Fragments arranged by size: 2310 2007 1026 807 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2886 10 Size: 54 1425 343 1054 3458 Fragments arranged by size: 3458 1425 1054 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2228 3047 4292 4651 4810 518 Size: 648 843 219 819 1245 359 159 2042 Fragments arranged by size: 2042 1245 843 819 648 359 219 159 MspA1I CmG'CkG Cuts at: 1606 2055 2458 2608 3356 3492 3750 4427 5575 Size: 449 403 150 748 136 258 677 1148 Cuts at: 5575 5820 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 748 677 449 403 258 245 150 136 NcoI C'CATG_G Cuts at: 513 1065 2004 2109 2392 513 Size: 552 939 105 283 4455 Fragments arranged by size: 4455 939 552 283 105 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NheI G'CTAG_C Cuts at: 1052 2075 1052 Size: 1023 5311 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 2452 2974 3349 3692 5237 1132 Size: 97 1223 522 375 343 1545 2229 Fragments arranged by size: 2229 1545 1223 522 375 343 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6334 PciI A'CATG_T Cuts at: 2448 5233 2448 Size: 2785 3549 PflMI CCAn_nnn'nTGG Cuts at: 1108 2260 2374 2836 1108 Size: 1152 114 462 4606 Fragments arranged by size: 4606 1152 462 114 PfoI T'CCnGG_A Cuts at: 3516 3516 Size: 6334 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PstI C_TGCA'G Cuts at: 830 2346 2613 3686 830 Size: 1516 267 1073 3478 Fragments arranged by size: 3478 1516 1073 267 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 2055 2458 2608 3356 3492 2055 Size: 403 150 748 136 4897 Fragments arranged by size: 4897 748 403 150 136 SacI G_AGCT'C Cuts at: 2208 2565 3464 2208 Size: 357 899 5078 Fragments arranged by size: 5078 899 357 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 4582 6294 4582 Size: 1712 4622 SfcI C'TryA_G Cuts at: 826 1045 2342 2609 3682 4817 5498 5689 826 Size: 219 1297 267 1073 1135 681 191 1471 Fragments arranged by size: 1471 1297 1135 1073 681 267 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 3061 3661 1872 Size: 1189 600 4545 Fragments arranged by size: 4545 1189 600 SmlI C'TyrA_G Cuts at: 820 1017 1943 2029 2172 4438 5339 5601 820 Size: 197 926 86 143 2266 901 262 1553 Fragments arranged by size: 2266 1553 926 901 262 197 143 86 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 3349 3692 1132 Size: 2217 343 3774 Fragments arranged by size: 3774 2217 343 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 3455 1517 Size: 1938 4396 StyI C'CwwG_G Cuts at: 513 1065 2004 2109 2392 3773 513 Size: 552 939 105 283 1381 3074 Fragments arranged by size: 3074 1381 939 552 283 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2139 3074 3537 4361 4378 4531 4716 Size: 1255 559 935 463 824 17 153 185 Cuts at: 4716 5161 325 Size: 445 1498 Fragments arranged by size: 1498 1255 935 824 559 463 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4580 6292 96 Size: 274 80 33 51 1443 2603 1712 138 Fragments arranged by size: 2603 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6294 6326 6294 Size: 32 6302 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 2194 3670 2194 Size: 1476 4858 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2596 2970 3079 2596 Size: 374 109 5851 Fragments arranged by size: 5851 374 109 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6334 XmaI C'CCGG_G Cuts at: 1870 3059 3659 1870 Size: 1189 600 4545 Fragments arranged by size: 4545 1189 600 XmnI GAAnn'nnTTC Cuts at: 4463 4463 Size: 6334 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI