(Linear) (Six-base) MAPSORT of: KIEE4135.seq Check: 1565 from: 1 to: 1629 KIEE4135.orf With 182 enzymes: SgfI * March 22, 2012 17:26 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 502 1403 1550 1629 Size: 328 174 901 147 79 Fragments arranged by size: 901 328 174 147 79 AflIII A'CryG_T Cuts at: 0 443 1629 Size: 443 1186 AleI CACnn'nnGTG Cuts at: 0 1042 1629 Size: 1042 587 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1108 1629 Size: 1108 521 AlwNI CAG_nnn'CTG Cuts at: 0 369 1186 1629 Size: 369 817 443 Fragments arranged by size: 817 443 369 ApaLI G'TGCA_C Cuts at: 0 787 1629 Size: 787 842 ApoI r'AATT_y Cuts at: 0 9 425 619 1629 Size: 9 416 194 1010 Fragments arranged by size: 1010 416 194 9 AvaI C'yCGr_G Cuts at: 0 1019 1054 1629 Size: 1019 35 575 Fragments arranged by size: 1019 575 35 BanI G'GyrC_C Cuts at: 0 1192 1629 Size: 1192 437 BanII G_rGCy'C Cuts at: 0 203 560 1459 1629 Size: 203 357 899 170 Fragments arranged by size: 899 357 203 170 BbsI GAAGACnn'nnnn_ Cuts at: 0 1500 1629 Size: 1500 129 BbvCI CC'TCA_GC Cuts at: 0 410 1629 Size: 410 1219 BglII A'GATC_T Cuts at: 0 1362 1629 Size: 1362 267 Bme1580I G_kGCm'C Cuts at: 0 791 1055 1629 Size: 791 264 574 Fragments arranged by size: 791 574 264 BmrI ACTGGGnnnn_n' Cuts at: 0 1005 1087 1629 Size: 1005 82 542 Fragments arranged by size: 1005 542 82 BmtI G_CTAG'C Cuts at: 0 74 1629 Size: 74 1555 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 492 1629 Size: 492 1137 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 1015 1473 1629 Size: 116 899 458 156 Fragments arranged by size: 899 458 156 116 Bpu10I CC'TnA_GC Cuts at: 0 410 554 1629 Size: 410 144 1075 Fragments arranged by size: 1075 410 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 9 152 1629 Size: 9 143 1477 Fragments arranged by size: 1477 143 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 103 208 211 427 1246 1629 Size: 103 105 3 216 819 383 Fragments arranged by size: 819 383 216 105 103 3 BseYI C'CCAG_C Cuts at: 0 599 1629 Size: 599 1030 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 872 1629 Size: 872 757 BsiHKAI G_wGCw'C Cuts at: 0 203 560 791 1011 1459 1629 Size: 203 357 231 220 448 170 Fragments arranged by size: 448 357 231 220 203 170 BsmI GAATG_Cn' Cuts at: 0 478 1629 Size: 478 1151 Bsp1286I G_dGCh'C Cuts at: 0 203 560 791 1011 1055 1459 1629 Size: 203 357 231 220 44 404 170 Fragments arranged by size: 404 357 231 220 203 170 44 BspHI T'CATG_A Cuts at: 0 176 1629 Size: 176 1453 BsrFI r'CCGG_y Cuts at: 0 1025 1629 Size: 1025 604 BstXI CCAn_nnnn'nTGG Cuts at: 0 225 1472 1629 Size: 225 1247 157 Fragments arranged by size: 1247 225 157 BstYI r'GATC_y Cuts at: 0 1362 1629 Size: 1362 267 Bsu36I CC'TnA_GG Cuts at: 0 15 1445 1629 Size: 15 1430 184 Fragments arranged by size: 1430 184 15 BtgI C'CryG_G Cuts at: 0 104 387 958 1629 Size: 104 283 571 671 Fragments arranged by size: 671 571 283 104 BtsI GCAGTG_nn' Cuts at: 0 905 1090 1629 Size: 905 185 539 Fragments arranged by size: 905 539 185 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 906 941 1629 Size: 906 35 688 Fragments arranged by size: 906 688 35 DraIII CAC_nnn'GTG Cuts at: 0 1081 1629 Size: 1081 548 EaeI y'GGCC_r Cuts at: 0 879 1629 Size: 879 750 EcoICRI GAG'CTC Cuts at: 0 201 558 1457 1629 Size: 201 357 899 172 Fragments arranged by size: 899 357 201 172 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 328 502 1015 1403 1473 1550 1629 Size: 116 212 174 513 388 70 77 79 Fragments arranged by size: 513 388 212 174 116 79 77 70 EcoNI CCTnn'n_nnAGG Cuts at: 0 310 1444 1629 Size: 310 1134 185 Fragments arranged by size: 1134 310 185 EcoRI G'AATT_C Cuts at: 0 9 1629 Size: 9 1620 EcoRV GAT'ATC Cuts at: 0 654 1629 Size: 654 975 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 755 1629 Size: 755 874 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 643 675 938 970 1090 1122 1629 Size: 643 32 263 32 120 32 507 Fragments arranged by size: 643 507 263 120 32 32 32 HincII GTy'rAC Cuts at: 0 993 1629 Size: 993 636 HindIII A'AGCT_T Cuts at: 0 762 1549 1629 Size: 762 787 80 Fragments arranged by size: 787 762 80 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 326 1133 1629 Size: 326 807 496 Fragments arranged by size: 807 496 326 MscI TGG'CCA Cuts at: 0 881 1629 Size: 881 748 MslI CAynn'nnrTG Cuts at: 0 223 1042 1629 Size: 223 819 587 Fragments arranged by size: 819 587 223 MspA1I CmG'CkG Cuts at: 0 50 453 603 1351 1487 1629 Size: 50 403 150 748 136 142 Fragments arranged by size: 748 403 150 142 136 50 NcoI C'CATG_G Cuts at: 0 104 387 1629 Size: 104 283 1242 Fragments arranged by size: 1242 283 104 NheI G'CTAG_C Cuts at: 0 70 1629 Size: 70 1559 NspI r_CATG'y Cuts at: 0 447 969 1344 1629 Size: 447 522 375 285 Fragments arranged by size: 522 447 375 285 PciI A'CATG_T Cuts at: 0 443 1629 Size: 443 1186 PflMI CCAn_nnn'nTGG Cuts at: 0 255 369 831 1629 Size: 255 114 462 798 Fragments arranged by size: 798 462 255 114 PfoI T'CCnGG_A Cuts at: 0 1511 1629 Size: 1511 118 PstI C_TGCA'G Cuts at: 0 341 608 1629 Size: 341 267 1021 Fragments arranged by size: 1021 341 267 PvuII CAG'CTG Cuts at: 0 50 453 603 1351 1487 1629 Size: 50 403 150 748 136 142 Fragments arranged by size: 748 403 150 142 136 50 SacI G_AGCT'C Cuts at: 0 203 560 1459 1629 Size: 203 357 899 170 Fragments arranged by size: 899 357 203 170 SfcI C'TryA_G Cuts at: 0 337 604 1629 Size: 337 267 1025 Fragments arranged by size: 1025 337 267 SmaI CCC'GGG Cuts at: 0 1056 1629 Size: 1056 573 SmlI C'TyrA_G Cuts at: 0 24 167 1629 Size: 24 143 1462 Fragments arranged by size: 1462 143 24 SphI G_CATG'C Cuts at: 0 1344 1629 Size: 1344 285 StuI AGG'CCT Cuts at: 0 1450 1629 Size: 1450 179 StyI C'CwwG_G Cuts at: 0 104 387 1629 Size: 104 283 1242 Fragments arranged by size: 1242 283 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 134 1069 1532 1629 Size: 134 935 463 97 Fragments arranged by size: 935 463 134 97 XbaI T'CTAG_A Cuts at: 0 189 1629 Size: 189 1440 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 591 965 1074 1629 Size: 591 374 109 555 Fragments arranged by size: 591 555 374 109 XmaI C'CCGG_G Cuts at: 0 1054 1629 Size: 1054 575 Enzymes that do cut: AcuI AflIII AleI AlfI AlwNI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrFI BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NheI NspI PciI PflMI PfoI PstI PvuII SacI SfcI SmaI SmlI SphI StuI StyI TaqII XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI DrdI EagI EarI EciI EcoO109I FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XhoI XmnI ZraI