(Circular) (Six-base) MAPSORT of: pFN21AB6522.seq Check: 6223 from: 1 to: 5866 pFN21AB6522 5866 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3209 1937 Size: 1272 4594 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2005 3174 3929 5313 5581 1632 Size: 373 1169 755 1384 268 1917 Fragments arranged by size: 1917 1384 1169 755 373 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 4765 5742 1203 Size: 3562 977 1327 Fragments arranged by size: 3562 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5866 AlwNI CAG_nnn'CTG Cuts at: 2760 2930 3609 5181 2760 Size: 170 679 1572 3445 Fragments arranged by size: 3445 1572 679 170 ApaLI G'TGCA_C Cuts at: 3923 5079 3923 Size: 1156 4710 ApoI r'AATT_y Cuts at: 1493 2189 2204 2372 2427 2605 2631 3175 3401 Size: 696 15 168 55 178 26 544 226 Cuts at: 3401 3553 5662 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 696 544 226 178 168 152 55 26 15 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 3191 1870 Size: 73 1248 4545 Fragments arranged by size: 4545 1248 73 BamHI G'GATC_C Cuts at: 3196 3563 3196 Size: 367 5499 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3187 4642 618 Size: 325 217 627 1400 1455 1842 Fragments arranged by size: 1842 1455 1400 627 325 217 BanII G_rGCy'C Cuts at: 1391 3185 1391 Size: 1794 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2424 928 Size: 971 525 4370 Fragments arranged by size: 4370 971 525 BbvCI CC'TCA_GC Cuts at: 3063 3063 Size: 5866 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3758 4968 3758 Size: 1210 4656 BclI T'GATC_A Cuts at: 1548 2392 1548 Size: 844 5022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4477 136 Size: 107 122 71 631 605 2805 1525 Fragments arranged by size: 2805 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 2489 3283 2489 Size: 794 5072 Bme1580I G_kGCm'C Cuts at: 1790 3927 5083 1790 Size: 2137 1156 2573 Fragments arranged by size: 2573 2137 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3597 4555 442 Size: 416 53 83 399 24 2180 958 1753 Fragments arranged by size: 2180 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2182 4526 1239 Size: 943 2344 2579 Fragments arranged by size: 2579 2344 943 Bpu10I CC'TnA_GC Cuts at: 3063 3063 Size: 5866 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3991 4856 5154 3991 Size: 865 298 4703 Fragments arranged by size: 4703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2862 4529 882 Size: 617 1363 1667 2219 Fragments arranged by size: 2219 1667 1363 617 BsaAI yAC'GTr Cuts at: 493 1204 2157 2827 3102 3657 493 Size: 711 953 670 275 555 2702 Fragments arranged by size: 2702 953 711 670 555 275 BsaBI GATnn'nnATC Cuts at: 2931 5860 2931 Size: 2929 2937 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3673 4055 4690 275 Size: 53 83 186 936 2140 382 635 1451 Fragments arranged by size: 2140 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4299 4697 4971 5118 5529 4299 Size: 398 274 147 411 4636 Fragments arranged by size: 4636 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 1178 Size: 30 165 30 597 30 5014 Fragments arranged by size: 5014 597 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2550 2570 2960 3080 1186 Size: 1364 20 390 120 3972 Fragments arranged by size: 3972 1364 390 120 20 BseYI C'CCAG_C Cuts at: 5069 5565 5069 Size: 496 5370 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2424 1163 Size: 573 688 4605 Fragments arranged by size: 4605 688 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3927 4012 5083 3927 Size: 85 1071 4710 Fragments arranged by size: 4710 1071 85 BsmI GAATG_Cn' Cuts at: 3377 3470 3377 Size: 93 5773 BsmBI CGTCTCn'nnnn_ Cuts at: 2212 2212 Size: 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3185 3927 4012 5083 1391 Size: 399 1395 742 85 1071 2174 Fragments arranged by size: 2174 1395 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 3755 5485 3755 Size: 1730 4136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3221 844 Size: 2377 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3753 1394 Size: 429 1930 3507 Fragments arranged by size: 3507 1930 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2288 4361 4535 65 Size: 1183 1040 2073 174 1396 Fragments arranged by size: 2073 1396 1183 1040 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4510 4697 1700 Size: 110 2700 187 2869 Fragments arranged by size: 2869 2700 187 110 BsrGI T'GTAC_A Cuts at: 96 2815 96 Size: 2719 3147 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 2181 3926 4938 2181 Size: 1745 1012 3109 Fragments arranged by size: 3109 1745 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5866 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5866 BstYI r'GATC_y Cuts at: 1972 2095 2713 3196 3563 3948 3965 5417 5503 Size: 123 618 483 367 385 17 1452 86 Cuts at: 5503 5515 5861 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 618 483 385 367 346 123 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4375 Fragments arranged by size: 4375 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 2436 3382 4176 4196 2436 Size: 946 794 20 4106 Fragments arranged by size: 4106 946 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 2091 3170 2091 Size: 1079 4787 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4873 809 Size: 507 3557 1802 Fragments arranged by size: 3557 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3313 4202 5831 Size: 54 1425 50 275 18 1483 889 1629 Cuts at: 5831 8 Size: 43 Fragments arranged by size: 1629 1483 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2105 2464 2703 2860 3796 1353 Size: 752 359 239 157 936 3423 Fragments arranged by size: 3423 936 752 359 239 157 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4453 4827 4973 387 Size: 1064 138 2864 374 146 1280 Fragments arranged by size: 2864 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2005 2182 3174 3929 4526 5313 5581 Size: 393 373 177 992 755 597 787 268 Cuts at: 5581 1239 Size: 1524 Fragments arranged by size: 1524 992 787 755 597 393 373 268 177 EcoO109I rG'GnC_Cy Cuts at: 2477 2800 2477 Size: 323 5543 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 EcoRV GAT'ATC Cuts at: 2225 3037 2225 Size: 812 5054 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2116 2252 2116 Size: 136 5730 FspI TGC'GCA Cuts at: 4372 4372 Size: 5866 HaeII r_GCGC'y Cuts at: 1678 5013 1678 Size: 3335 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2209 2241 2606 Size: 32 135 32 428 32 177 32 365 Cuts at: 2606 2638 4507 4539 4581 4613 1373 Size: 32 1869 32 42 32 2626 Fragments arranged by size: 2626 1869 428 365 177 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3210 3456 669 Size: 988 281 1272 246 3079 Fragments arranged by size: 3079 1272 988 281 246 HindIII A'AGCT_T Cuts at: 748 2445 748 Size: 1697 4169 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2423 4980 5164 1305 Size: 1118 2557 184 2007 Fragments arranged by size: 2557 2007 1118 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4044 Fragments arranged by size: 4044 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2567 3081 3824 4183 4342 518 Size: 648 843 558 514 743 359 159 2042 Fragments arranged by size: 2042 843 743 648 558 514 359 159 MspA1I CmG'CkG Cuts at: 1606 2745 3282 3959 5107 5352 1606 Size: 1139 537 677 1148 245 2120 Fragments arranged by size: 2120 1148 1139 677 537 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4375 Fragments arranged by size: 4375 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 3224 4769 1132 Size: 97 1995 1545 2229 Fragments arranged by size: 2229 1995 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5866 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5866 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2477 2477 Size: 5866 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 2637 3436 2637 Size: 799 5067 PstI C_TGCA'G Cuts at: 830 3218 830 Size: 2388 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 PvuII CAG'CTG Cuts at: 2745 2745 Size: 5866 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SfcI C'TryA_G Cuts at: 826 1045 3214 4349 5030 5221 826 Size: 219 2169 1135 681 191 1471 Fragments arranged by size: 2169 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 3193 1872 Size: 1321 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3970 4871 5133 820 Size: 197 926 2027 901 262 1553 Fragments arranged by size: 2027 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2157 3657 493 Size: 1664 1500 2702 Fragments arranged by size: 2702 1664 1500 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 3224 1132 Size: 2092 3774 SspI AAT'ATT Cuts at: 5 52 2654 3790 5 Size: 47 2602 1136 2081 Fragments arranged by size: 2602 2081 1136 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5866 StyI C'CwwG_G Cuts at: 513 1065 2004 3305 513 Size: 552 939 1301 3074 Fragments arranged by size: 3074 1301 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2840 3893 3910 4063 4248 4693 325 Size: 1255 1260 1053 17 153 185 445 1498 Fragments arranged by size: 1498 1260 1255 1053 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2815 4112 5824 Size: 274 80 33 51 1443 838 1297 1712 Cuts at: 5824 96 Size: 138 Fragments arranged by size: 1712 1443 1297 838 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5826 5858 5826 Size: 32 5834 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3136 3136 Size: 5866 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5866 XmaI C'CCGG_G Cuts at: 1870 3191 1870 Size: 1321 4545 XmnI GAAnn'nnTTC Cuts at: 2018 3995 2018 Size: 1977 3889 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI