Restriction map for
pFN21AE0677
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DpnII DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI HincII HindII HindIII HpaI
KpnI KspI MamI MboI MfeI
MflI MluI MroI MseI MslI
MunI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
Sau3AI ScaI SexAI SfaNI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 640 CGCG CG^CG
AcyI 720 GGCGCC G[GA]^CG[CT]C
ApaI 528 GGGCCC GGGCC^C
AvaI 142 CTCGAG C^[CT]CG[GA]G
AviII 626 TGCGCA TGC^GCA
BalI 617 TGGCCA TGG^CCA
BanI 720 GGCGCC G^G[CT][GA]CC
BbeI 720 GGCGCC GGCGC^C
BfaI 28 CTAG C^TAG
BsaHI 720 GGCGCC G[GA]^CG[CT]C
BsaWI 107 ACCGGA [AT]^CCGG[AT]
BseRI 332 CTCCTC GAGGAG(10/8)
BsgI 229 GTGCAG GTGCAG(16/14)
BsoBI 142 CTCGAG C^[CT]CG[GA]G
BspMI 231 GCAGGT ACCTGC(4/8)
BsrFI 717 GCCGGC [GA]^CCGG[CT]
BsrI 337 CCAGT ACTGG(1/-1)
BstUI 640 CGCG CG^CG
Cfr10I 717 GCCGGC [GA]^CCGG[CT]
FspI 626 TGCGCA TGC^GCA
HgaI 548 GCGTC GACGC(5/10)
Hin1I 720 GGCGCC G[GA]^CG[CT]C
KasI 720 GGCGCC G^GCGCC
MaeI 28 CTAG C^TAG
MaeII 115 ACGT A^CGT
MluNI 617 TGGCCA TGG^CCA
MscI 617 TGGCCA TGG^CCA
MspA1I 130 CAGCTG C[AC]G^C[GT]G
MvnI 640 CGCG CG^CG
NaeI 717 GCCGGC GCC^GGC
NarI 720 GGCGCC GG^CGCC
NgoMIV 717 GCCGGC G^CCGGC
PaeR7I 142 CTCGAG C^TCGAG
PpuMI 429 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 130 CAGCTG CAG^CTG
SacI 193 GAGCTC GAGCT^C
SapI 190 GAAGAGC GCTCTTC(1/4)
SbfI 433 CCTGCAGG CCTGCA^GG
SfiI 609 GGCCAGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 720 GGCGCC GGC^GCC
SmlI 142 CTCGAG C^T[CT][GA]AG
Sse8387I 433 CCTGCAGG CCTGCA^GG
StuI 497 AGGCCT AGG^CCT
TaqI 143 TCGA T^CGA
Tsp45I 325 GTGAC ^GT[GC]AC
TthHB8I 143 TCGA T^CGA
XcmI 305 CCAAGGAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 142 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 369 GGACC G^G[AT]CC
430 GGTCC
BbsI 267 GAAGAC GAAGAC(2/6)
178 GTCTTC
BglI 610 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
712 GCCAAGCCGGC
BpmI 335 CTCCAG CTGGAG(16/14)
397 CTGGAG
BpuAI 267 GAAGAC GAAGAC(2/6)
178 GTCTTC
DdeI 285 CTCAG C^T[ACGT]AG
648 CTCAG
DsaI 314 CCATGG C^C[GA][CT]GG
560 CCATGG
HinfI 468 GACTC G^A[ACGT]TC
566 GACTC
HphI 324 GGTGA GGTGA(8/7)
182 TCACC
MaeIII 325 GTGAC ^GT[ACGT]AC
599 GTAAC
NcoI 314 CCATGG C^CATGG
560 CCATGG
PleI 468 GACTC GAGTC(4/5)
566 GACTC
PstI 434 CTGCAG CTGCA^G
661 CTGCAG
SfcI 434 CTGCAG C^T[GA][CT]AG
661 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwNI AspHI BanII
BbvI BcnI BmyI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI Ksp632I
MboII MnlI MspI MvaI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
StyI TseI TspRI