(Circular) (Six-base) MAPSORT of: pFN21AE0677.seq Check: 3340 from: 1 to: 5494 pFN21AE0677 5494 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3304 278 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2837 1937 Size: 900 4594 Acc65I G'GTAC_C Cuts at: 2815 2815 Size: 5494 AclI AA'CG_TT Cuts at: 1563 3621 3994 1563 Size: 2058 373 3063 Fragments arranged by size: 3063 2058 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 3557 4941 5209 1632 Size: 482 1443 1384 268 1917 Fragments arranged by size: 1917 1443 1384 482 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5297 AflIII A'CryG_T Cuts at: 1203 4393 5370 1203 Size: 3190 977 1327 Fragments arranged by size: 3190 1327 977 AgeI A'CCGG_T Cuts at: 4325 4325 Size: 5494 AhdI GACnn_n'nnGTC Cuts at: 4223 4223 Size: 5494 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5494 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5494 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2419 2451 2419 Size: 32 5462 AlwNI CAG_nnn'CTG Cuts at: 2137 2419 2660 3237 4809 2137 Size: 282 241 577 1572 2822 Fragments arranged by size: 2822 1572 577 282 241 ApaI G_GGCC'C Cuts at: 2537 2537 Size: 5494 ApaLI G'TGCA_C Cuts at: 3551 4707 3551 Size: 1156 4338 ApoI r'AATT_y Cuts at: 1493 2803 3029 3181 5290 1493 Size: 1310 226 152 2109 1697 Fragments arranged by size: 2109 1697 1310 226 152 AseI AT'TA_AT Cuts at: 160 1032 4048 160 Size: 872 3016 1606 Fragments arranged by size: 3016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 AvaI C'yCGr_G Cuts at: 1870 1943 2147 2819 1870 Size: 73 204 672 4545 Fragments arranged by size: 4545 672 204 73 BamHI G'GATC_C Cuts at: 2824 3191 2824 Size: 367 5127 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2725 2815 4270 618 Size: 325 217 627 938 90 1455 1842 Fragments arranged by size: 1842 1455 938 627 325 217 90 BanII G_rGCy'C Cuts at: 1391 2202 2319 2537 2813 1391 Size: 811 117 218 276 4072 Fragments arranged by size: 4072 811 276 218 117 BbeI G_GCGC'C Cuts at: 2729 2729 Size: 5494 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2176 2279 928 Size: 971 277 103 4143 Fragments arranged by size: 4143 971 277 103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3685 3719 1903 Size: 34 1748 34 3678 Fragments arranged by size: 3678 1748 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3386 4596 3386 Size: 1210 4284 BclI T'GATC_A Cuts at: 1548 1548 Size: 5494 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2621 2723 4105 Size: 107 122 71 631 605 949 102 1382 Cuts at: 4105 136 Size: 1525 Fragments arranged by size: 1525 1382 949 631 605 122 107 102 71 BglII A'GATC_T Cuts at: 5489 5489 Size: 5494 BlpI GC'TnA_GC Cuts at: 2911 2911 Size: 5494 Bme1580I G_kGCm'C Cuts at: 1790 2042 2537 3555 4711 1790 Size: 252 495 1018 1156 2573 Fragments arranged by size: 2573 1156 1018 495 252 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5494 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3225 4183 442 Size: 416 53 83 399 24 1808 958 1753 Fragments arranged by size: 1808 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5494 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2216 2216 Size: 5494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2325 2423 4154 1239 Size: 1086 98 1731 2579 Fragments arranged by size: 2579 1731 1086 98 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3619 4484 4782 3619 Size: 865 298 4331 Fragments arranged by size: 4331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4157 882 Size: 617 2658 2219 Fragments arranged by size: 2658 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3285 493 Size: 711 2081 2702 Fragments arranged by size: 2702 2081 711 BsaBI GATnn'nnATC Cuts at: 5488 5488 Size: 5494 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2726 3301 3683 4318 Size: 53 83 186 936 1193 575 382 635 Cuts at: 4318 275 Size: 1451 Fragments arranged by size: 1451 1193 936 635 575 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2112 3927 4325 4599 4746 5157 2112 Size: 1815 398 274 147 411 2449 Fragments arranged by size: 2449 1815 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2323 2342 2353 2372 1178 Size: 30 165 30 920 19 11 19 4300 Fragments arranged by size: 4300 920 165 30 30 19 19 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2328 1186 Size: 1142 4352 BseYI C'CCAG_C Cuts at: 2409 4697 5193 2409 Size: 2288 496 2710 Fragments arranged by size: 2710 2288 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2255 1163 Size: 573 519 4402 Fragments arranged by size: 4402 573 519 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2944 3705 4733 1540 Size: 275 186 943 761 1028 2301 Fragments arranged by size: 2301 1028 943 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2202 2546 2652 3555 3640 4711 2202 Size: 344 106 903 85 1071 2985 Fragments arranged by size: 2985 1071 903 344 106 85 BsmI GAATG_Cn' Cuts at: 3005 3098 3005 Size: 93 5401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2202 2319 2537 2546 2652 2813 Size: 399 252 160 117 218 9 106 161 Cuts at: 2813 3555 3640 4711 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 252 218 161 160 117 106 85 9 BspEI T'CCGG_A Cuts at: 5157 5157 Size: 5494 BspHI T'CATG_A Cuts at: 3383 5113 3383 Size: 1730 3764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2227 2849 844 Size: 1383 622 3489 Fragments arranged by size: 3489 1383 622 BsrBI CCG'CTC Cuts at: 1394 1823 3381 1394 Size: 429 1558 3507 Fragments arranged by size: 3507 1558 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3989 4163 65 Size: 1183 2741 174 1396 Fragments arranged by size: 2741 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2722 4138 4325 1700 Size: 110 912 1416 187 2869 Fragments arranged by size: 2869 1416 912 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5494 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5494 BssSI C'ACGA_G Cuts at: 3554 4566 3554 Size: 1012 4482 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5494 BstBI TT'CG_AA Cuts at: 4298 4298 Size: 5494 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5494 BstYI r'GATC_y Cuts at: 1972 2824 3191 3576 3593 5045 5131 5143 5489 Size: 852 367 385 17 1452 86 12 346 Cuts at: 5489 1972 Size: 1977 Fragments arranged by size: 1977 1452 852 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2319 2565 513 Size: 552 1254 246 3442 Fragments arranged by size: 3442 1254 552 246 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4755 Fragments arranged by size: 4755 683 56 BtsI GCAGTG_nn' Cuts at: 2017 2040 3010 3804 3824 2017 Size: 23 970 794 20 3687 Fragments arranged by size: 3687 970 794 23 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5494 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5459 DraI TTT'AAA Cuts at: 2798 2798 Size: 5494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4501 809 Size: 507 3185 1802 Fragments arranged by size: 3185 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2487 2613 2622 Size: 54 1425 50 275 18 657 126 9 Cuts at: 2622 2941 3830 5459 8 Size: 319 889 1629 43 Fragments arranged by size: 1629 1425 889 657 319 275 126 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2941 1537 Size: 275 1129 4090 Fragments arranged by size: 4090 1129 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2068 2133 2190 2780 3424 1353 Size: 715 65 57 590 644 3423 Fragments arranged by size: 3423 715 644 590 65 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4081 4455 4601 387 Size: 1064 138 2492 374 146 1280 Fragments arranged by size: 2492 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2200 2200 Size: 5494 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2325 2423 3557 4154 4941 5209 Size: 393 482 211 98 1134 597 787 268 Cuts at: 5209 1239 Size: 1524 Fragments arranged by size: 1524 1134 787 597 482 393 268 211 98 EcoO109I rG'GnC_Cy Cuts at: 2286 2435 2444 2533 2286 Size: 149 9 89 5247 Fragments arranged by size: 5247 149 89 9 EcoRI G'AATT_C Cuts at: 2803 3181 2803 Size: 378 5116 FspI TGC'GCA Cuts at: 2633 4000 2633 Size: 1367 4127 HaeII r_GCGC'y Cuts at: 1678 2107 2402 2729 4641 1678 Size: 429 295 327 1912 2531 Fragments arranged by size: 2531 1912 429 327 295 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2110 2142 2323 2355 4135 Size: 32 135 32 538 32 181 32 1780 Cuts at: 4135 4167 4209 4241 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1780 538 181 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2838 3084 669 Size: 988 281 900 246 3079 Fragments arranged by size: 3079 988 900 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5494 HpaI GTT'AAC Cuts at: 3084 3084 Size: 5494 KasI G'GCGC_C Cuts at: 2725 2725 Size: 5494 KpnI G_GTAC'C Cuts at: 2819 2819 Size: 5494 MfeI C'AATT_G Cuts at: 3093 3093 Size: 5494 MluI A'CGCG_T Cuts at: 5370 5370 Size: 5494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4608 4792 1305 Size: 3303 184 2007 Fragments arranged by size: 3303 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2624 10 Size: 54 1425 343 792 2880 Fragments arranged by size: 2880 1425 792 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3452 3811 3970 518 Size: 648 2286 359 159 2042 Fragments arranged by size: 2286 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2910 3587 4735 4980 1606 Size: 531 773 677 1148 245 2120 Fragments arranged by size: 2120 1148 773 677 531 245 NaeI GCC'GGC Cuts at: 2724 2724 Size: 5494 NarI GG'CG_CC Cuts at: 2726 2726 Size: 5494 NcoI C'CATG_G Cuts at: 513 1065 2319 2565 513 Size: 552 1254 246 3442 Fragments arranged by size: 3442 1254 552 246 NdeI CA'TA_TG Cuts at: 387 387 Size: 5494 NgoMIV G'CCGG_C Cuts at: 2722 2722 Size: 5494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5494 NotI GC'GGCC_GC Cuts at: 2941 2941 Size: 5494 NspI r_CATG'y Cuts at: 1132 1229 2852 4397 1132 Size: 97 1623 1545 2229 Fragments arranged by size: 2229 1623 1545 97 PasI CC'CwG_GG Cuts at: 1348 2369 1348 Size: 1021 4473 PciI A'CATG_T Cuts at: 4393 4393 Size: 5494 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5494 PmeI GTTT'AAAC Cuts at: 2798 2798 Size: 5494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 5076 5108 3563 Size: 32 1481 32 3949 Fragments arranged by size: 3949 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2435 2435 Size: 5494 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5494 PsiI TTA'TAA Cuts at: 3064 3064 Size: 5494 PspOMI G'GGCC_C Cuts at: 2533 2533 Size: 5494 PstI C_TGCA'G Cuts at: 830 2443 2670 2846 830 Size: 1613 227 176 3478 Fragments arranged by size: 3478 1613 227 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5494 PvuII CAG'CTG Cuts at: 2137 2137 Size: 5494 SacI G_AGCT'C Cuts at: 2202 2202 Size: 5494 SalI G'TCGA_C Cuts at: 1936 2836 1936 Size: 900 4594 SapI GCTCTTCn'nnn_ Cuts at: 2190 2190 Size: 5494 SbfI CC_TGCA'GG Cuts at: 2443 2846 2443 Size: 403 5091 ScaI AGT'ACT Cuts at: 3742 5454 3742 Size: 1712 3782 SfcI C'TryA_G Cuts at: 826 1045 2439 2666 2842 3977 4658 4849 826 Size: 219 1394 227 176 1135 681 191 1471 Fragments arranged by size: 1471 1394 1135 681 227 219 191 176 SfiI GGCCn_nnn'nGGCC Cuts at: 2621 2621 Size: 5494 SfoI GGC'GCC Cuts at: 2727 2727 Size: 5494 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5494 SmaI CCC'GGG Cuts at: 1872 2821 1872 Size: 949 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 3598 4499 4761 820 Size: 197 926 204 1451 901 262 1553 Fragments arranged by size: 1553 1451 926 901 262 204 197 SnaBI TAC'GTA Cuts at: 493 3285 493 Size: 2792 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5494 SphI G_CATG'C Cuts at: 1132 2852 1132 Size: 1720 3774 SspI AAT'ATT Cuts at: 5 52 3418 5 Size: 47 3366 2081 Fragments arranged by size: 3366 2081 47 StuI AGG'CCT Cuts at: 1517 2504 1517 Size: 987 4507 StyI C'CwwG_G Cuts at: 513 1065 2310 2319 2565 2763 2933 513 Size: 552 1245 9 246 198 170 3074 Fragments arranged by size: 3074 1245 552 246 198 170 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3521 3538 3691 3876 4321 325 Size: 1255 1941 17 153 185 445 1498 Fragments arranged by size: 1941 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3740 5452 96 Size: 274 80 33 51 1443 1763 1712 138 Fragments arranged by size: 1763 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5454 5486 5454 Size: 32 5462 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5494 XbaI T'CTAG_A Cuts at: 2830 2830 Size: 5494 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2317 2317 Size: 5494 XhoI C'TCGA_G Cuts at: 1943 2147 1943 Size: 204 5290 XmaI C'CCGG_G Cuts at: 1870 2819 1870 Size: 949 4545 XmnI GAAnn'nnTTC Cuts at: 3623 3623 Size: 5494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3302 276 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI