Restriction map for
pFN21AE0677
(Linear) (Six-base) MAPSORT of: KIEE0677.seq Check: 2138 from: 1 to: 789
KIEE0677.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 789
Size: 109 680
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 414 446 789
Size: 414 32 343
Fragments arranged by size:
414 343 32
AlwNI CAG_nnn'CTG
Cuts at: 0 132 414 655 789
Size: 132 282 241 134
Fragments arranged by size:
282 241 134 132
ApaI G_GGCC'C
Cuts at: 0 532 789
Size: 532 257
AvaI C'yCGr_G
Cuts at: 0 142 789
Size: 142 647
BanI G'GyrC_C
Cuts at: 0 720 789
Size: 720 69
BanII G_rGCy'C
Cuts at: 0 197 314 532 789
Size: 197 117 218 257
Fragments arranged by size:
257 218 197 117
BbeI G_GCGC'C
Cuts at: 0 724 789
Size: 724 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 171 274 789
Size: 171 103 515
Fragments arranged by size:
515 171 103
BglI GCCn_nnn'nGGC
Cuts at: 0 616 718 789
Size: 616 102 71
Fragments arranged by size:
616 102 71
Bme1580I G_kGCm'C
Cuts at: 0 37 532 789
Size: 37 495 257
Fragments arranged by size:
495 257 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 211 789
Size: 211 578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 418 789
Size: 320 98 371
Fragments arranged by size:
371 320 98
BsaHI Gr'CG_yC
Cuts at: 0 721 789
Size: 721 68
BsaWI w'CCGG_w
Cuts at: 0 107 789
Size: 107 682
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 318 337 348 367 789
Size: 318 19 11 19 422
Fragments arranged by size:
422 318 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 323 789
Size: 323 466
BseYI C'CCAG_C
Cuts at: 0 404 789
Size: 404 385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 789
Size: 250 539
BsiHKAI G_wGCw'C
Cuts at: 0 197 541 647 789
Size: 197 344 106 142
Fragments arranged by size:
344 197 142 106
Bsp1286I G_dGCh'C
Cuts at: 0 37 197 314 532 541 647 789
Size: 37 160 117 218 9 106 142
Fragments arranged by size:
218 160 142 117 106 37 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 222 789
Size: 222 567
BsrFI r'CCGG_y
Cuts at: 0 717 789
Size: 717 72
BtgI C'CryG_G
Cuts at: 0 314 560 789
Size: 314 246 229
Fragments arranged by size:
314 246 229
BtsI GCAGTG_nn'
Cuts at: 0 12 35 789
Size: 12 23 754
Fragments arranged by size:
754 23 12
EaeI y'GGCC_r
Cuts at: 0 482 608 617 789
Size: 482 126 9 172
Fragments arranged by size:
482 172 126 9
EarI CTCTTCn'nnn_
Cuts at: 0 63 128 185 775 789
Size: 63 65 57 590 14
Fragments arranged by size:
590 65 63 57 14
EcoICRI GAG'CTC
Cuts at: 0 195 789
Size: 195 594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 320 418 789
Size: 109 211 98 371
Fragments arranged by size:
371 211 109 98
EcoO109I rG'GnC_Cy
Cuts at: 0 281 430 439 528 789
Size: 281 149 9 89 261
Fragments arranged by size:
281 261 149 89 9
FspI TGC'GCA
Cuts at: 0 628 789
Size: 628 161
HaeII r_GCGC'y
Cuts at: 0 102 397 724 789
Size: 102 295 327 65
Fragments arranged by size:
327 295 102 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 318 350 789
Size: 105 32 181 32 439
Fragments arranged by size:
439 181 105 32 32
KasI G'GCGC_C
Cuts at: 0 720 789
Size: 720 69
MscI TGG'CCA
Cuts at: 0 619 789
Size: 619 170
MspA1I CmG'CkG
Cuts at: 0 132 789
Size: 132 657
NaeI GCC'GGC
Cuts at: 0 719 789
Size: 719 70
NarI GG'CG_CC
Cuts at: 0 721 789
Size: 721 68
NcoI C'CATG_G
Cuts at: 0 314 560 789
Size: 314 246 229
Fragments arranged by size:
314 246 229
NgoMIV G'CCGG_C
Cuts at: 0 717 789
Size: 717 72
PasI CC'CwG_GG
Cuts at: 0 364 789
Size: 364 425
PpuMI rG'GwC_Cy
Cuts at: 0 430 789
Size: 430 359
PspOMI G'GGCC_C
Cuts at: 0 528 789
Size: 528 261
PstI C_TGCA'G
Cuts at: 0 438 665 789
Size: 438 227 124
Fragments arranged by size:
438 227 124
PvuII CAG'CTG
Cuts at: 0 132 789
Size: 132 657
SacI G_AGCT'C
Cuts at: 0 197 789
Size: 197 592
SapI GCTCTTCn'nnn_
Cuts at: 0 185 789
Size: 185 604
SbfI CC_TGCA'GG
Cuts at: 0 438 789
Size: 438 351
SfcI C'TryA_G
Cuts at: 0 434 661 789
Size: 434 227 128
Fragments arranged by size:
434 227 128
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 616 789
Size: 616 173
SfoI GGC'GCC
Cuts at: 0 722 789
Size: 722 67
SmlI C'TyrA_G
Cuts at: 0 142 789
Size: 142 647
StuI AGG'CCT
Cuts at: 0 499 789
Size: 499 290
StyI C'CwwG_G
Cuts at: 0 305 314 560 758 789
Size: 305 9 246 198 31
Fragments arranged by size:
305 246 198 31 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 312 789
Size: 312 477
XhoI C'TCGA_G
Cuts at: 0 142 789
Size: 142 647
Enzymes that do cut:
AcuI AloI AlwNI ApaI AvaI BanI BanII BbeI
BbsI BglI Bme1580I BplI BpmI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BtgI
BtsI EaeI EarI EcoICRI Eco57MI EcoO109I FspI HaeII
Hin4I KasI MscI MspA1I NaeI NarI NcoI NgoMIV
PasI PpuMI PspOMI PstI PvuII SacI SapI SbfI
SfcI SfiI SfoI SmlI StuI StyI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MslI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XmaI XmnI ZraI