Restriction map for
pFN21AE0713
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII AccI AccII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AviII AvrII BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KspI
MaeI MaeII MamI MfeI MluI
MseI MspA1I MunI MvnI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 857 GGTACC G^GTACC
AccIII 272 TCCGGA T^CCGGA
AocI 560 CCTCAGG CC^T[ACGT]AGG
Asp718I 857 GGTACC G^GTACC
BamHI 41 GGATCC G^GATCC
BbeI 115 GGCGCC GGCGC^C
BglI 709 GCCTGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 272 TCCGGA [AT]^CCGG[AT]
BseAI 272 TCCGGA T^CCGGA
BsgI 682 CTGCAC GTGCAG(16/14)
BsmAI 457 GAGAC GTCTC(1/5)
BspEI 272 TCCGGA T^CCGGA
BspHI 807 TCATGA T^CATGA
BsrI 735 CCAGT ACTGG(1/-1)
BstAPI 684 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 151 GGATG GGATG(2/0)
BstXI 474 CCATACTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 41 GGATCC [GA]^GATC[CT]
Bsu36I 560 CCTCAGG CC^T[ACGT]AGG
DpnII 42 GATC ^GATC
EarI 640 CTCTTC CTCTTC(1/4)
Eco81I 560 CCTCAGG CC^T[ACGT]AGG
EcoNI 576 CCTACGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 626 CCCGC CCCGC(4/6)
FokI 151 GGATG GGATG(9/13)
HapII 273 CCGG C^CGG
HgaI 539 GACGC GACGC(5/10)
HpaII 273 CCGG C^CGG
KasI 115 GGCGCC G^GCGCC
KpnI 857 GGTACC GGTAC^C
Ksp632I 640 CTCTTC CTCTTC(1/4)
MaeIII 849 GTCAC ^GT[ACGT]AC
MboI 42 GATC ^GATC
MflI 41 GGATCC [GA]^GATC[CT]
MroI 272 TCCGGA T^CCGGA
MslI 645 CACTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 273 CCGG C^CGG
NarI 115 GGCGCC GG^CGCC
NdeII 42 GATC ^GATC
NspI 629 GCATGT [GA]CATG^[CT]
PflMI 162 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 241 CTGCAG CTGCA^G
RcaI 807 TCATGA T^CATGA
Sau3AI 42 GATC ^GATC
SbfI 240 CCTGCAGG CCTGCA^GG
ScaI 29 AGTACT AGT^ACT
SfaNI 152 GATGC GCATC(5/9)
SfcI 241 CTGCAG C^T[GA][CT]AG
SfoI 115 GGCGCC GGC^GCC
Sse8387I 240 CCTGCAGG CCTGCA^GG
TfiI 372 GAATC G^A[AT]TC
Tsp45I 849 GTCAC ^GT[GC]AC
Tsp509I 206 AATT ^AATT
Van91I 162 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 81 CCAGGCCCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 41 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcyI 115 GGCGCC G[GA]^CG[CT]C
539 GACGCC
AlwI 42 GATCC GGATC(4/5)
41 GGATC
BalI 662 TGGCCA TGG^CCA
759 TGGCCA
BanII 103 GAGCTC G[GA]GC[CT]^C
193 GAGCTC
BsaHI 115 GGCGCC G[GA]^CG[CT]C
539 GACGCC
BseRI 655 CTCCTC GAGGAG(10/8)
841 GAGGAG
BspMI 332 ACCTGC ACCTGC(4/8)
686 ACCTGC
DsaI 609 CCATGG C^C[GA][CT]GG
728 CCATGG
EaeI 662 TGGCCA [CT]^GGCC[GA]
759 TGGCCA
Hin1I 115 GGCGCC G[GA]^CG[CT]C
539 GACGCC
MluNI 662 TGGCCA TGG^CCA
759 TGGCCA
MscI 662 TGGCCA TGG^CCA
759 TGGCCA
NcoI 609 CCATGG C^CATGG
728 CCATGG
PleI 8 GAGTC GAGTC(4/5)
470 GAGTC
SacI 103 GAGCTC GAGCT^C
193 GAGCTC
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanI BbvI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
EcoT14I Fnu4HI HaeII HaeIII HhaI
HinP1I HinfI ItaI MboII MnlI
MvaI MwoI NlaIII NlaIV PpuMI
RsaI Sau96I ScrFI StyI TseI
TspRI