(Circular) (Six-base) MAPSORT of: pFN21AE0713.seq Check: 3159 from: 1 to: 5602 pFN21AE0713 5602 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2700 2700 Size: 5602 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2945 1937 Size: 1008 4594 Acc65I G'GTAC_C Cuts at: 2862 2923 2862 Size: 61 5541 AclI AA'CG_TT Cuts at: 1563 2901 3729 4102 1563 Size: 1338 828 373 3063 Fragments arranged by size: 3063 1338 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2279 2309 3665 5049 5317 1632 Size: 647 30 1356 1384 268 1917 Fragments arranged by size: 1917 1384 1356 647 268 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5405 AflIII A'CryG_T Cuts at: 1203 4501 5478 1203 Size: 3298 977 1327 Fragments arranged by size: 3298 1327 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 2654 1166 Size: 1488 4114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5602 AlwNI CAG_nnn'CTG Cuts at: 2092 2134 2338 2533 3345 4917 2092 Size: 42 204 195 812 1572 2777 Fragments arranged by size: 2777 1572 812 204 195 42 ApaLI G'TGCA_C Cuts at: 3659 4815 3659 Size: 1156 4446 ApoI r'AATT_y Cuts at: 1493 2911 3137 3289 5398 1493 Size: 1418 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 226 152 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2927 1870 Size: 73 984 4545 Fragments arranged by size: 4545 984 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2846 2854 2879 2887 2846 Size: 8 25 8 5561 Fragments arranged by size: 5561 25 8 8 BamHI G'GATC_C Cuts at: 2046 2932 3299 2046 Size: 886 367 4349 Fragments arranged by size: 4349 886 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2605 2705 2862 2923 Size: 325 217 627 333 485 100 157 61 Cuts at: 2923 4378 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 485 333 325 217 157 100 61 BanII G_rGCy'C Cuts at: 1391 2112 2202 2921 1391 Size: 721 90 719 4072 Fragments arranged by size: 4072 721 719 90 BbeI G_GCGC'C Cuts at: 2124 2124 Size: 5602 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4631 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3793 3827 1903 Size: 34 50 34 1772 34 3678 Fragments arranged by size: 3678 1772 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3494 4704 3494 Size: 1210 4392 BclI T'GATC_A Cuts at: 1548 1548 Size: 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2720 4213 136 Size: 107 122 71 631 605 1048 1493 1525 Fragments arranged by size: 1525 1493 1048 631 605 122 107 71 BglII A'GATC_T Cuts at: 5597 5597 Size: 5602 BlpI GC'TnA_GC Cuts at: 3019 3019 Size: 5602 Bme1580I G_kGCm'C Cuts at: 1790 3663 4819 1790 Size: 1873 1156 2573 Fragments arranged by size: 2573 1873 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2734 3333 4291 Size: 416 53 83 399 24 1317 599 958 Cuts at: 4291 442 Size: 1753 Fragments arranged by size: 1753 1317 958 599 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 2315 2348 4262 1239 Size: 956 120 33 1914 2579 Fragments arranged by size: 2579 1914 956 120 33 Bpu10I CC'TnA_GC Cuts at: 2079 2079 Size: 5602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3727 4592 4890 3727 Size: 865 298 4439 Fragments arranged by size: 4439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4265 882 Size: 617 2766 2219 Fragments arranged by size: 2766 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3393 493 Size: 711 2189 2702 Fragments arranged by size: 2702 2189 711 BsaBI GATnn'nnATC Cuts at: 5596 5596 Size: 5602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2121 2545 3409 3791 Size: 53 83 186 936 588 424 864 382 Cuts at: 3791 4426 275 Size: 635 1451 Fragments arranged by size: 1451 936 864 635 588 424 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2277 4035 4433 4707 4854 5265 2277 Size: 1758 398 274 147 411 2614 Fragments arranged by size: 2614 1758 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2837 2867 1178 Size: 30 165 30 1434 30 3913 Fragments arranged by size: 3913 1434 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2651 2861 1186 Size: 1465 210 3927 Fragments arranged by size: 3927 1465 210 BseYI C'CCAG_C Cuts at: 2008 2160 4805 5301 2008 Size: 152 2645 496 2309 Fragments arranged by size: 2645 2309 496 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2672 1163 Size: 573 936 4093 Fragments arranged by size: 4093 936 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3052 3813 4841 1540 Size: 275 186 1051 761 1028 2301 Fragments arranged by size: 2301 1051 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2112 2202 2661 3663 3748 4819 2112 Size: 90 459 1002 85 1071 2895 Fragments arranged by size: 2895 1071 1002 459 90 85 BsmI GAATG_Cn' Cuts at: 3113 3206 3113 Size: 93 5509 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2202 2661 2921 3663 3748 4819 Size: 399 322 90 459 260 742 85 1071 Cuts at: 4819 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 459 399 322 260 90 85 BspEI T'CCGG_A Cuts at: 2277 5265 2277 Size: 2988 2614 BspHI T'CATG_A Cuts at: 2812 3491 5221 2812 Size: 679 1730 3193 Fragments arranged by size: 3193 1730 679 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 2700 2957 844 Size: 1502 354 257 3489 Fragments arranged by size: 3489 1502 354 257 BsrBI CCG'CTC Cuts at: 1394 1823 3489 1394 Size: 429 1666 3507 Fragments arranged by size: 3507 1666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4097 4271 65 Size: 1183 2849 174 1396 Fragments arranged by size: 2849 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4246 4433 1700 Size: 110 2436 187 2869 Fragments arranged by size: 2869 2436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 2695 794 Size: 1901 3701 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5602 BstXI CCAn_nnnn'nTGG Cuts at: 2486 2486 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2046 2932 3299 3684 3701 5153 5239 5251 Size: 74 886 367 385 17 1452 86 12 Cuts at: 5251 5597 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 886 385 367 346 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 2566 2566 Size: 5602 BtgI C'CryG_G Cuts at: 513 1065 2614 2733 513 Size: 552 1549 119 3382 Fragments arranged by size: 3382 1549 552 119 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4863 Fragments arranged by size: 4863 683 56 BtsI GCAGTG_nn' Cuts at: 2684 3118 3912 3932 2684 Size: 434 794 20 4354 Fragments arranged by size: 4354 794 434 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5567 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2667 2764 3049 Size: 54 1425 50 275 18 837 97 285 Cuts at: 3049 3938 5567 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 837 285 275 97 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2651 3532 1353 Size: 1298 881 3423 Fragments arranged by size: 3423 1298 881 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4189 4563 4709 387 Size: 1064 138 2600 374 146 1280 Fragments arranged by size: 2600 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2110 2200 2110 Size: 90 5512 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2279 2309 2315 2348 3665 4262 Size: 393 563 84 30 6 33 1317 597 Cuts at: 4262 5049 5317 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1317 787 597 563 393 268 84 33 30 6 EcoNI CCTnn'n_nnAGG Cuts at: 2585 2585 Size: 5602 EcoO109I rG'GnC_Cy Cuts at: 2064 2089 2232 2320 2335 2408 2722 2777 2064 Size: 25 143 88 15 73 314 55 4889 Fragments arranged by size: 4889 314 143 88 73 55 25 15 EcoRI G'AATT_C Cuts at: 2911 3289 2911 Size: 378 5224 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 2086 2124 2575 4749 1678 Size: 408 38 451 2174 2531 Fragments arranged by size: 2531 2174 451 408 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2837 2869 4243 4275 4317 Size: 32 135 32 1265 32 1374 32 42 Cuts at: 4317 4349 1373 Size: 32 2626 Fragments arranged by size: 2626 1374 1265 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2946 3192 669 Size: 988 281 1008 246 3079 Fragments arranged by size: 3079 1008 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5602 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KasI G'GCGC_C Cuts at: 2120 2120 Size: 5602 KpnI G_GTAC'C Cuts at: 2866 2927 2866 Size: 61 5541 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4716 4900 1305 Size: 3411 184 2007 Fragments arranged by size: 3411 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2669 2766 10 Size: 54 1425 343 837 97 2846 Fragments arranged by size: 2846 1425 837 343 97 54 MslI CAynn'nnrTG Cuts at: 518 1166 2654 3560 3919 4078 518 Size: 648 1488 906 359 159 2042 Fragments arranged by size: 2042 1488 906 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3018 3695 4843 5088 1606 Size: 1412 677 1148 245 2120 Fragments arranged by size: 2120 1412 1148 677 245 NarI GG'CG_CC Cuts at: 2121 2121 Size: 5602 NcoI C'CATG_G Cuts at: 513 1065 2614 2733 513 Size: 552 1549 119 3382 Fragments arranged by size: 3382 1549 552 119 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2638 2960 4505 1132 Size: 97 1409 322 1545 2229 Fragments arranged by size: 2229 1545 1409 322 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5602 PciI A'CATG_T Cuts at: 4501 4501 Size: 5602 PflMI CCAn_nnn'nTGG Cuts at: 1108 2173 1108 Size: 1065 4537 PfoI T'CCnGG_A Cuts at: 2331 2331 Size: 5602 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3671 3703 5184 5216 3671 Size: 32 1481 32 4057 Fragments arranged by size: 4057 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2064 2232 2335 2408 2064 Size: 168 103 73 5258 Fragments arranged by size: 5258 168 103 73 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 3172 3172 Size: 5602 PstI C_TGCA'G Cuts at: 830 2250 2954 830 Size: 1420 704 3478 Fragments arranged by size: 3478 1420 704 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5602 SacI G_AGCT'C Cuts at: 2112 2202 2112 Size: 90 5512 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SanDI GG'GwC_CC Cuts at: 2064 2064 Size: 5602 SbfI CC_TGCA'GG Cuts at: 2250 2954 2250 Size: 704 4898 ScaI AGT'ACT Cuts at: 2036 3850 5562 2036 Size: 1814 1712 2076 Fragments arranged by size: 2076 1814 1712 SfcI C'TryA_G Cuts at: 826 1045 2246 2950 4085 4766 4957 826 Size: 219 1201 704 1135 681 191 1471 Fragments arranged by size: 1471 1201 1135 704 681 219 191 SfoI GGC'GCC Cuts at: 2122 2122 Size: 5602 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2929 1872 Size: 1057 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3706 4607 4869 820 Size: 197 926 1763 901 262 1553 Fragments arranged by size: 1763 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5602 StyI C'CwwG_G Cuts at: 513 1065 2058 2167 2614 2733 3041 513 Size: 552 993 109 447 119 308 3074 Fragments arranged by size: 3074 993 552 447 308 119 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3629 3646 3799 3984 4429 325 Size: 1255 2049 17 153 185 445 1498 Fragments arranged by size: 2049 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2034 3848 5560 Size: 274 80 33 51 1443 57 1814 1712 Cuts at: 5560 96 Size: 138 Fragments arranged by size: 1814 1712 1443 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5562 5594 5562 Size: 32 5570 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2093 2093 Size: 5602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2927 1870 Size: 1057 4545 XmnI GAAnn'nnTTC Cuts at: 3731 3731 Size: 5602 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI