Restriction map for
pFN21AE0713
(Linear) (Six-base) MAPSORT of: KIEE0713.seq Check: 604 from: 1 to: 897
KIEE0713.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 695 897
Size: 695 202
Acc65I G'GTAC_C
Cuts at: 0 857 897
Size: 857 40
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 304 897
Size: 274 30 593
Fragments arranged by size:
593 274 30
AleI CACnn'nnGTG
Cuts at: 0 649 897
Size: 649 248
AlwNI CAG_nnn'CTG
Cuts at: 0 87 129 333 528 897
Size: 87 42 204 195 369
Fragments arranged by size:
369 204 195 87 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 841 849 874 882 897
Size: 841 8 25 8 15
Fragments arranged by size:
841 25 15 8 8
BamHI G'GATC_C
Cuts at: 0 41 897
Size: 41 856
BanI G'GyrC_C
Cuts at: 0 115 600 700 857 897
Size: 115 485 100 157 40
Fragments arranged by size:
485 157 115 100 40
BanII G_rGCy'C
Cuts at: 0 107 197 897
Size: 107 90 700
Fragments arranged by size:
700 107 90
BbeI G_GCGC'C
Cuts at: 0 119 897
Size: 119 778
BglI GCCn_nnn'nGGC
Cuts at: 0 715 897
Size: 715 182
BmrI ACTGGGnnnn_n'
Cuts at: 0 729 897
Size: 729 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 310 343 897
Size: 190 120 33 554
Fragments arranged by size:
554 190 120 33
Bpu10I CC'TnA_GC
Cuts at: 0 74 897
Size: 74 823
BsaHI Gr'CG_yC
Cuts at: 0 116 540 897
Size: 116 424 357
Fragments arranged by size:
424 357 116
BsaWI w'CCGG_w
Cuts at: 0 272 897
Size: 272 625
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 832 862 897
Size: 832 30 35
Fragments arranged by size:
832 35 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 646 856 897
Size: 646 210 41
Fragments arranged by size:
646 210 41
BseYI C'CCAG_C
Cuts at: 0 3 155 897
Size: 3 152 742
Fragments arranged by size:
742 152 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 897
Size: 667 230
BsiHKAI G_wGCw'C
Cuts at: 0 107 197 656 897
Size: 107 90 459 241
Fragments arranged by size:
459 241 107 90
Bsp1286I G_dGCh'C
Cuts at: 0 107 197 656 897
Size: 107 90 459 241
Fragments arranged by size:
459 241 107 90
BspEI T'CCGG_A
Cuts at: 0 272 897
Size: 272 625
BspHI T'CATG_A
Cuts at: 0 807 897
Size: 807 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 695 897
Size: 341 354 202
Fragments arranged by size:
354 341 202
BstAPI GCAn_nnn'nTGC
Cuts at: 0 690 897
Size: 690 207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 481 897
Size: 481 416
BstYI r'GATC_y
Cuts at: 0 41 897
Size: 41 856
Bsu36I CC'TnA_GG
Cuts at: 0 561 897
Size: 561 336
BtgI C'CryG_G
Cuts at: 0 609 728 897
Size: 609 119 169
Fragments arranged by size:
609 169 119
BtsI GCAGTG_nn'
Cuts at: 0 679 897
Size: 679 218
EaeI y'GGCC_r
Cuts at: 0 662 759 897
Size: 662 97 138
Fragments arranged by size:
662 138 97
EarI CTCTTCn'nnn_
Cuts at: 0 646 897
Size: 646 251
EcoICRI GAG'CTC
Cuts at: 0 105 195 897
Size: 105 90 702
Fragments arranged by size:
702 105 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 274 304 310 343 897
Size: 190 84 30 6 33 554
Fragments arranged by size:
554 190 84 33 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 580 897
Size: 580 317
EcoO109I rG'GnC_Cy
Cuts at: 0 59 84 227 315 330 403 717 772
Size: 59 25 143 88 15 73 314 55
Cuts at: 772 897
Size: 125
Fragments arranged by size:
314 143 125 88 73 59 55 25
15
HaeII r_GCGC'y
Cuts at: 0 81 119 570 897
Size: 81 38 451 327
Fragments arranged by size:
451 327 81 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 832 864 897
Size: 832 32 33
Fragments arranged by size:
832 33 32
KasI G'GCGC_C
Cuts at: 0 115 897
Size: 115 782
KpnI G_GTAC'C
Cuts at: 0 861 897
Size: 861 36
MscI TGG'CCA
Cuts at: 0 664 761 897
Size: 664 97 136
Fragments arranged by size:
664 136 97
MslI CAynn'nnrTG
Cuts at: 0 649 897
Size: 649 248
NarI GG'CG_CC
Cuts at: 0 116 897
Size: 116 781
NcoI C'CATG_G
Cuts at: 0 609 728 897
Size: 609 119 169
Fragments arranged by size:
609 169 119
NspI r_CATG'y
Cuts at: 0 633 897
Size: 633 264
PflMI CCAn_nnn'nTGG
Cuts at: 0 168 897
Size: 168 729
PfoI T'CCnGG_A
Cuts at: 0 326 897
Size: 326 571
PpuMI rG'GwC_Cy
Cuts at: 0 59 227 330 403 897
Size: 59 168 103 73 494
Fragments arranged by size:
494 168 103 73 59
PstI C_TGCA'G
Cuts at: 0 245 897
Size: 245 652
SacI G_AGCT'C
Cuts at: 0 107 197 897
Size: 107 90 700
Fragments arranged by size:
700 107 90
SanDI GG'GwC_CC
Cuts at: 0 59 897
Size: 59 838
SbfI CC_TGCA'GG
Cuts at: 0 245 897
Size: 245 652
ScaI AGT'ACT
Cuts at: 0 31 897
Size: 31 866
SfcI C'TryA_G
Cuts at: 0 241 897
Size: 241 656
SfoI GGC'GCC
Cuts at: 0 117 897
Size: 117 780
StyI C'CwwG_G
Cuts at: 0 53 162 609 728 897
Size: 53 109 447 119 169
Fragments arranged by size:
447 169 119 109 53
TatI w'GTAC_w
Cuts at: 0 29 897
Size: 29 868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 88 897
Size: 88 809
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI BaeI BamHI BanI
BanII BbeI BglI BmrI BpmI Bpu10I BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
KpnI MscI MslI NarI NcoI NspI PflMI PfoI
PpuMI PstI SacI SanDI SbfI ScaI SfcI SfoI
StyI TatI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
MfeI MluI MmeI MspA1I NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SapI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI
ZraI