(Circular) (Six-base) MAPSORT of: pFN21AE0648.seq Check: 7768 from: 1 to: 5845 pFN21AE0648 5845 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3027 3188 1937 Size: 1090 161 4594 Fragments arranged by size: 4594 1090 161 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 2237 2605 3908 5292 5560 1632 Size: 578 27 368 1303 1384 268 1917 Fragments arranged by size: 1917 1384 1303 578 368 268 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 4744 5721 1203 Size: 3541 977 1327 Fragments arranged by size: 3541 1327 977 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 2944 4574 2944 Size: 1630 4215 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5845 AlwNI CAG_nnn'CTG Cuts at: 2065 2611 3588 5160 2065 Size: 546 977 1572 2750 Fragments arranged by size: 2750 1572 977 546 ApaI G_GGCC'C Cuts at: 2826 3139 2826 Size: 313 5532 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 3154 3380 3532 5641 1493 Size: 1661 226 152 2109 1697 Fragments arranged by size: 2109 1697 1661 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 2699 2724 3170 1870 Size: 73 756 25 446 4545 Fragments arranged by size: 4545 756 446 73 25 BamHI G'GATC_C Cuts at: 3175 3542 3175 Size: 367 5478 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2318 2558 2880 3088 3166 Size: 325 217 627 531 240 322 208 78 Cuts at: 3166 4621 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 531 325 322 240 217 208 78 BanII G_rGCy'C Cuts at: 1391 2201 2585 2646 2826 3139 3164 1391 Size: 810 384 61 180 313 25 4072 Fragments arranged by size: 4072 810 384 313 180 61 25 BbeI G_GCGC'C Cuts at: 2322 2562 3092 2322 Size: 240 530 5075 Fragments arranged by size: 5075 530 240 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2258 928 Size: 971 359 4515 Fragments arranged by size: 4515 971 359 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2504 2538 4036 4070 1903 Size: 34 567 34 1498 34 3678 Fragments arranged by size: 3678 1498 567 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2372 2736 4456 Size: 107 122 71 631 605 700 364 1720 Cuts at: 4456 136 Size: 1525 Fragments arranged by size: 1720 1525 700 631 605 364 122 107 71 BglII A'GATC_T Cuts at: 5840 5840 Size: 5845 BlpI GC'TnA_GC Cuts at: 2567 3262 2567 Size: 695 5150 Bme1580I G_kGCm'C Cuts at: 1790 2826 3139 3906 5062 1790 Size: 1036 313 767 1156 2573 Fragments arranged by size: 2573 1156 1036 767 313 BmgBI CAC'GTC Cuts at: 1318 2115 1318 Size: 797 5048 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3576 4534 442 Size: 416 53 83 399 24 2159 958 1753 Fragments arranged by size: 2159 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4505 1239 Size: 3266 2579 Bpu10I CC'TnA_GC Cuts at: 2543 2543 Size: 5845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3970 4835 5133 3970 Size: 865 298 4682 Fragments arranged by size: 4682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2657 4508 882 Size: 617 1158 1851 2219 Fragments arranged by size: 2219 1851 1158 617 BsaAI yAC'GTr Cuts at: 493 1204 2170 2758 3636 493 Size: 711 966 588 878 2702 Fragments arranged by size: 2702 966 878 711 588 BsaBI GATnn'nnATC Cuts at: 5839 5839 Size: 5845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2292 2319 2559 3089 Size: 53 83 186 936 759 27 240 530 Cuts at: 3089 3652 4034 4669 275 Size: 563 382 635 1451 Fragments arranged by size: 1451 936 759 635 563 530 382 240 186 83 53 27 BsaWI w'CCGG_w Cuts at: 4278 4676 4950 5097 5508 4278 Size: 398 274 147 411 4615 Fragments arranged by size: 4615 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2375 2405 1178 Size: 30 165 30 972 30 4618 Fragments arranged by size: 4618 972 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2519 2651 2654 1186 Size: 1333 132 3 4377 Fragments arranged by size: 4377 1333 132 3 BseYI C'CCAG_C Cuts at: 2049 2282 2954 5048 5544 2049 Size: 233 672 2094 496 2350 Fragments arranged by size: 2350 2094 672 496 233 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2455 2771 3029 1163 Size: 573 719 316 258 3979 Fragments arranged by size: 3979 719 573 316 258 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2326 3295 4056 5084 1540 Size: 275 186 325 969 761 1028 2301 Fragments arranged by size: 2301 1028 969 761 325 275 186 BsiHKAI G_wGCw'C Cuts at: 2646 3111 3906 3991 5062 2646 Size: 465 795 85 1071 3429 Fragments arranged by size: 3429 1071 795 465 85 BsmI GAATG_Cn' Cuts at: 3356 3449 3356 Size: 93 5752 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 2585 2646 2826 3111 3139 3164 Size: 399 411 384 61 180 285 28 25 Cuts at: 3164 3906 3991 5062 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 411 399 384 285 180 85 61 28 25 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 3734 5464 3734 Size: 1730 4115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3133 3200 844 Size: 2289 67 3489 Fragments arranged by size: 3489 2289 67 BsrBI CCG'CTC Cuts at: 1394 1823 2086 2176 2380 2908 3732 1394 Size: 429 263 90 204 528 824 3507 Fragments arranged by size: 3507 824 528 429 263 204 90 BsrDI GCAATG_nn' Cuts at: 65 1248 2578 4340 4514 65 Size: 1183 1330 1762 174 1396 Fragments arranged by size: 1762 1396 1330 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4489 4676 1700 Size: 110 2679 187 2869 Fragments arranged by size: 2869 2679 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5845 BssHII G'CGCG_C Cuts at: 1925 2259 1925 Size: 334 5511 BssSI C'ACGA_G Cuts at: 2204 3014 3905 4917 2204 Size: 810 891 1012 3132 Fragments arranged by size: 3132 1012 891 810 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5845 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5845 BstXI CCAn_nnnn'nTGG Cuts at: 2170 2170 Size: 5845 BstYI r'GATC_y Cuts at: 1972 3175 3542 3927 3944 5396 5482 5494 5840 Size: 1203 367 385 17 1452 86 12 346 Cuts at: 5840 1972 Size: 1977 Fragments arranged by size: 1977 1452 1203 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2667 3057 513 Size: 552 1602 390 3301 Fragments arranged by size: 3301 1602 552 390 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5106 Fragments arranged by size: 5106 683 56 BtsI GCAGTG_nn' Cuts at: 3361 4155 4175 3361 Size: 794 20 5031 Fragments arranged by size: 5031 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2373 2575 Size: 54 1425 50 275 18 306 237 202 Cuts at: 2575 3292 4181 5810 8 Size: 717 889 1629 43 Fragments arranged by size: 1629 1425 889 717 306 275 237 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3292 1537 Size: 275 1480 4090 Fragments arranged by size: 4090 1480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3775 1353 Size: 2422 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2644 2644 Size: 5845 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2237 2605 3908 4505 5292 5560 Size: 393 578 27 368 1303 597 787 268 Cuts at: 5560 1239 Size: 1524 Fragments arranged by size: 1524 1303 787 597 578 393 368 268 27 EcoNI CCTnn'n_nnAGG Cuts at: 2192 2402 2552 2192 Size: 210 150 5485 Fragments arranged by size: 5485 210 150 EcoO109I rG'GnC_Cy Cuts at: 2823 3135 3136 2823 Size: 312 1 5532 Fragments arranged by size: 5532 312 1 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 FspI TGC'GCA Cuts at: 2552 2796 4351 2552 Size: 244 1555 4046 Fragments arranged by size: 4046 1555 244 HaeII r_GCGC'y Cuts at: 1678 2322 2562 2842 3049 3092 4992 1678 Size: 644 240 280 207 43 1900 2531 Fragments arranged by size: 2531 1900 644 280 240 207 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 2930 2962 4486 Size: 32 135 32 806 32 520 32 1524 Cuts at: 4486 4518 4560 4592 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1524 806 520 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3189 3435 669 Size: 988 281 1251 246 3079 Fragments arranged by size: 3079 1251 988 281 246 HindIII A'AGCT_T Cuts at: 748 2536 748 Size: 1788 4057 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KasI G'GCGC_C Cuts at: 2318 2558 3088 2318 Size: 240 530 5075 Fragments arranged by size: 5075 530 240 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2187 2514 4959 5143 1305 Size: 882 327 2445 184 2007 Fragments arranged by size: 2445 2007 882 327 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2577 10 Size: 54 1425 343 745 3278 Fragments arranged by size: 3278 1425 745 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 2672 3803 4162 4321 518 Size: 648 1257 249 1131 359 159 2042 Fragments arranged by size: 2042 1257 1131 648 359 249 159 MspA1I CmG'CkG Cuts at: 1606 2316 2835 3043 3261 3938 5086 5331 1606 Size: 710 519 208 218 677 1148 245 2120 Fragments arranged by size: 2120 1148 710 677 519 245 218 208 NarI GG'CG_CC Cuts at: 2319 2559 3089 2319 Size: 240 530 5075 Fragments arranged by size: 5075 530 240 NcoI C'CATG_G Cuts at: 513 1065 2667 3057 513 Size: 552 1602 390 3301 Fragments arranged by size: 3301 1602 552 390 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 2600 3203 4748 1132 Size: 97 1371 603 1545 2229 Fragments arranged by size: 2229 1545 1371 603 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5845 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 2065 2115 3058 1108 Size: 957 50 943 3895 Fragments arranged by size: 3895 957 943 50 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PspOMI G'GGCC_C Cuts at: 2822 3135 2822 Size: 313 5532 PstI C_TGCA'G Cuts at: 830 3144 3197 830 Size: 2314 53 3478 Fragments arranged by size: 3478 2314 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 SacI G_AGCT'C Cuts at: 2646 2646 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SbfI CC_TGCA'GG Cuts at: 3144 3197 3144 Size: 53 5792 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SfcI C'TryA_G Cuts at: 826 1045 3140 3193 4328 5009 5200 826 Size: 219 2095 53 1135 681 191 1471 Fragments arranged by size: 2095 1471 1135 681 219 191 53 SfiI GGCCn_nnn'nGGCC Cuts at: 2736 2736 Size: 5845 SfoI GGC'GCC Cuts at: 2320 2560 3090 2320 Size: 240 530 5075 Fragments arranged by size: 5075 530 240 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 2726 3172 1872 Size: 854 446 4545 Fragments arranged by size: 4545 854 446 SmlI C'TyrA_G Cuts at: 820 1017 1943 3949 4850 5112 820 Size: 197 926 2006 901 262 1553 Fragments arranged by size: 2006 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 2232 1517 Size: 715 5130 StyI C'CwwG_G Cuts at: 513 1065 2667 3057 3284 513 Size: 552 1602 390 227 3074 Fragments arranged by size: 3074 1602 552 390 227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3872 3889 4042 4227 4672 325 Size: 1255 2292 17 153 185 445 1498 Fragments arranged by size: 2292 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4091 5803 96 Size: 274 80 33 51 1443 2114 1712 138 Fragments arranged by size: 2114 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5805 5837 5805 Size: 32 5813 Tth111I GACn'n_nGTC Cuts at: 1316 3023 1316 Size: 1707 4138 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2497 2755 3064 2497 Size: 258 309 5278 Fragments arranged by size: 5278 309 258 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 2724 3170 1870 Size: 854 446 4545 Fragments arranged by size: 4545 854 446 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SrfI SwaI