(Circular) (Six-base) MAPSORT of: pFN21AE0684.seq Check: 5664 from: 1 to: 5902 pFN21AE0684 5902 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2307 3712 278 Size: 53 83 186 936 771 1405 2468 Fragments arranged by size: 2468 1405 936 771 186 83 53 AccI GT'mk_AC Cuts at: 1937 3245 1937 Size: 1308 4594 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2064 2455 2840 3965 5349 5617 1632 Size: 432 391 385 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1125 432 391 385 268 AflII C'TTAA_G Cuts at: 820 1017 2449 820 Size: 197 1432 4273 Fragments arranged by size: 4273 1432 197 AflIII A'CryG_T Cuts at: 1203 2568 3162 4801 5778 1203 Size: 1365 594 1639 977 1327 Fragments arranged by size: 1639 1365 1327 977 594 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5902 AlwNI CAG_nnn'CTG Cuts at: 3645 5217 3645 Size: 1572 4330 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 2231 2256 2378 2716 3211 3437 3589 5698 Size: 738 25 122 338 495 226 152 2109 Cuts at: 5698 1493 Size: 1697 Fragments arranged by size: 2109 1697 738 495 338 226 152 122 25 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 2480 2546 3091 3227 1870 Size: 73 537 66 545 136 4545 Fragments arranged by size: 4545 545 537 136 73 66 BamHI G'GATC_C Cuts at: 2242 3232 3599 2242 Size: 990 367 4545 Fragments arranged by size: 4545 990 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3223 4678 618 Size: 325 217 627 1436 1455 1842 Fragments arranged by size: 1842 1455 1436 627 325 217 BanII G_rGCy'C Cuts at: 1391 2916 2991 3098 3221 1391 Size: 1525 75 107 123 4072 Fragments arranged by size: 4072 1525 123 107 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2019 2052 2307 2545 2846 928 Size: 971 120 33 255 238 301 3984 Fragments arranged by size: 3984 971 301 255 238 120 33 BbvCI CC'TCA_GC Cuts at: 2711 2942 2711 Size: 231 5671 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 1548 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4513 136 Size: 107 122 71 631 605 2841 1525 Fragments arranged by size: 2841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5897 5897 Size: 5902 BlpI GC'TnA_GC Cuts at: 3319 3319 Size: 5902 Bme1580I G_kGCm'C Cuts at: 1790 3963 5119 1790 Size: 2173 1156 2573 Fragments arranged by size: 2573 2173 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2305 3633 4591 Size: 416 53 83 399 24 888 1328 958 Cuts at: 4591 442 Size: 1753 Fragments arranged by size: 1753 1328 958 888 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4562 1239 Size: 3323 2579 Bpu10I CC'TnA_GC Cuts at: 2711 2942 2711 Size: 231 5671 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4027 4892 5190 4027 Size: 865 298 4739 Fragments arranged by size: 4739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4565 882 Size: 617 3066 2219 Fragments arranged by size: 3066 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2571 2578 3693 493 Size: 711 1367 7 1115 2702 Fragments arranged by size: 2702 1367 1115 711 7 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 3709 4091 4726 Size: 53 83 186 936 771 1405 382 635 Cuts at: 4726 275 Size: 1451 Fragments arranged by size: 1451 1405 936 771 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3078 4335 4733 5007 5154 5565 3078 Size: 1257 398 274 147 411 3415 Fragments arranged by size: 3415 1257 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2820 2850 1178 Size: 30 165 30 1417 30 4230 Fragments arranged by size: 4230 1417 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 2717 3116 3125 1186 Size: 1009 522 399 9 3963 Fragments arranged by size: 3963 1009 522 399 9 BseYI C'CCAG_C Cuts at: 2824 2999 5105 5601 2824 Size: 175 2106 496 3125 Fragments arranged by size: 3125 2106 496 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2803 3049 3352 4113 5141 1540 Size: 275 186 802 246 303 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 303 275 246 186 BsiHKAI G_wGCw'C Cuts at: 2991 3963 4048 5119 2991 Size: 972 85 1071 3774 Fragments arranged by size: 3774 1071 972 85 BsmI GAATG_Cn' Cuts at: 3413 3506 3413 Size: 93 5809 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2916 2991 3098 3221 3963 4048 5119 Size: 399 1126 75 107 123 742 85 1071 Cuts at: 5119 1391 Size: 2174 Fragments arranged by size: 2174 1126 1071 742 399 123 107 85 75 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2289 3004 3257 844 Size: 1445 715 253 3489 Fragments arranged by size: 3489 1445 715 253 BsrBI CCG'CTC Cuts at: 1394 1823 3789 1394 Size: 429 1966 3507 Fragments arranged by size: 3507 1966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4397 4571 65 Size: 1183 3149 174 1396 Fragments arranged by size: 3149 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2853 4546 4733 1700 Size: 110 1043 1693 187 2869 Fragments arranged by size: 2869 1693 1043 187 110 BsrGI T'GTAC_A Cuts at: 96 2440 96 Size: 2344 3558 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5902 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 2242 2751 3081 3148 3232 3599 3984 4001 Size: 270 509 330 67 84 367 385 17 Cuts at: 4001 5453 5539 5551 5897 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 509 385 367 346 330 270 86 84 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2700 2700 Size: 5902 BtgI C'CryG_G Cuts at: 513 1065 2004 2217 2643 2671 513 Size: 552 939 213 426 28 3744 Fragments arranged by size: 3744 939 552 426 213 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2644 487 Size: 683 56 1418 3745 Fragments arranged by size: 3745 1418 683 56 BtsI GCAGTG_nn' Cuts at: 3418 4212 4232 3418 Size: 794 20 5088 Fragments arranged by size: 5088 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DraIII CAC_nnn'GTG Cuts at: 2629 2629 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3046 3349 4238 Size: 54 1425 50 275 18 1216 303 889 Cuts at: 4238 5867 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1216 889 303 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3046 3349 1537 Size: 275 1234 303 4090 Fragments arranged by size: 4090 1234 303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2055 3832 1353 Size: 702 1777 3423 Fragments arranged by size: 3423 1777 702 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4489 4863 5009 387 Size: 1064 138 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2989 2989 Size: 5902 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2064 2455 2840 3965 4562 5349 5617 Size: 393 432 391 385 1125 597 787 268 Cuts at: 5617 1239 Size: 1524 Fragments arranged by size: 1524 1125 787 597 432 393 391 385 268 EcoNI CCTnn'n_nnAGG Cuts at: 2699 2699 Size: 5902 EcoO109I rG'GnC_Cy Cuts at: 2692 2962 2692 Size: 270 5632 EcoRI G'AATT_C Cuts at: 2256 3211 3589 2256 Size: 955 378 4569 Fragments arranged by size: 4569 955 378 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 2854 5049 1678 Size: 1176 2195 2531 Fragments arranged by size: 2531 2195 1176 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2149 2181 4543 4575 4617 Size: 32 135 32 577 32 2362 32 42 Cuts at: 4617 4649 1373 Size: 32 2626 Fragments arranged by size: 2626 2362 577 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3246 3492 669 Size: 988 281 1308 246 3079 Fragments arranged by size: 3079 1308 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5902 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2913 5016 5200 1305 Size: 1608 2103 184 2007 Fragments arranged by size: 2103 2007 1608 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4080 Fragments arranged by size: 4080 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2597 2663 3860 4219 4378 518 Size: 648 1431 66 1197 359 159 2042 Fragments arranged by size: 2042 1431 1197 648 359 159 66 MspA1I CmG'CkG Cuts at: 1606 2673 3318 3995 5143 5388 1606 Size: 1067 645 677 1148 245 2120 Fragments arranged by size: 2120 1148 1067 677 645 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2217 513 Size: 552 939 213 4198 Fragments arranged by size: 4198 939 552 213 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 3166 3260 4805 1132 Size: 97 1937 94 1545 2229 Fragments arranged by size: 2229 1937 1545 97 94 PasI CC'CwG_GG Cuts at: 1348 2916 1348 Size: 1568 4334 PciI A'CATG_T Cuts at: 3162 4801 3162 Size: 1639 4263 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5902 PfoI T'CCnGG_A Cuts at: 2754 2754 Size: 5902 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PmlI CAC'GTG Cuts at: 2571 2571 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2692 2962 2692 Size: 270 5632 PshAI GACnn'nnGTC Cuts at: 1613 2085 1613 Size: 472 5430 PsiI TTA'TAA Cuts at: 2322 3472 2322 Size: 1150 4752 PstI C_TGCA'G Cuts at: 830 3254 830 Size: 2424 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 SacI G_AGCT'C Cuts at: 2991 2991 Size: 5902 SacII CC_GC'GG Cuts at: 2674 2674 Size: 5902 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2285 3250 4385 5066 5257 826 Size: 219 1240 965 1135 681 191 1471 Fragments arranged by size: 1471 1240 1135 965 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 2853 1700 Size: 1153 4749 SmaI CCC'GGG Cuts at: 1872 2482 2548 3229 1872 Size: 610 66 681 4545 Fragments arranged by size: 4545 681 610 66 SmlI C'TyrA_G Cuts at: 820 1017 1943 2449 4006 4907 5169 820 Size: 197 926 506 1557 901 262 1553 Fragments arranged by size: 1557 1553 926 901 506 262 197 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 2386 3826 5 Size: 47 2334 1440 2081 Fragments arranged by size: 2334 2081 1440 47 StuI AGG'CCT Cuts at: 1517 2334 3026 1517 Size: 817 692 4393 Fragments arranged by size: 4393 817 692 StyI C'CwwG_G Cuts at: 513 1065 2004 2217 3341 513 Size: 552 939 213 1124 3074 Fragments arranged by size: 3074 1124 939 552 213 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 2885 3929 3946 4099 4284 4729 Size: 1255 774 531 1044 17 153 185 445 Cuts at: 4729 325 Size: 1498 Fragments arranged by size: 1498 1255 1044 774 531 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2440 4148 5860 Size: 274 80 33 51 1443 463 1708 1712 Cuts at: 5860 96 Size: 138 Fragments arranged by size: 1712 1708 1443 463 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2820 2852 5862 5894 2820 Size: 32 3010 32 2828 Fragments arranged by size: 3010 2828 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 2077 3238 2077 Size: 1161 4741 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 2480 2546 3227 1870 Size: 610 66 681 4545 Fragments arranged by size: 4545 681 610 66 XmnI GAAnn'nnTTC Cuts at: 2073 4031 2073 Size: 1958 3944 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2305 3710 276 Size: 53 83 186 936 771 1405 2468 Fragments arranged by size: 2468 1405 936 771 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI