Restriction map for
pFN21AB7725
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII BalI BamHI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII Bst1107I BstAPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRV FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaII KasI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 310 GGTACC G^GTACC
AflIII 1024 ACATGT A^C[GA][CT]GT
AlwNI 693 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 310 GGTACC G^GTACC
AspHI 76 GAGCTC G[AT]GC[AT]^C
AvaII 159 GGACC G^G[AT]CC
AvrII 244 CCTAGG C^CTAGG
BanI 310 GGTACC G^G[CT][GA]CC
BanII 76 GAGCTC G[GA]GC[CT]^C
BbsI 457 GTCTTC GAAGAC(2/6)
BcgI 366 GCATGTTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 244 CCTAGG C^CTAGG
BmyI 76 GAGCTC G[AGT]GC[ACT]^C
BpuAI 457 GTCTTC GAAGAC(2/6)
BsgI 1110 GTGCAG GTGCAG(16/14)
BsiHKAI 76 GAGCTC G[AT]GC[AT]^C
BsmFI 185 GGGAC GGGAC(10/14)
Bsp1286I 76 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 179 TGTACA T^GTACA
BspLU11I 1024 ACATGT A^CATGT
BsrGI 179 TGTACA T^GTACA
BssSI 885 CACGAG CACGAG(-5/-1)
BstBI 374 TTCGAA TT^CGAA
BstEII 105 GGTGACC G^GT[ACGT]ACC
BstF5I 117 GGATG GGATG(2/0)
BstPI 105 GGTGACC G^GT[ACGT]ACC
BstXI 945 CCACGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 89 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 159 GGACC G^G[ACGT]CC
DsaI 125 CCGTGG C^C[GA][CT]GG
EaeI 122 TGGCCG [CT]^GGCC[GA]
EarI 289 GAAGAG CTCTTC(1/4)
EcoNI 962 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 105 GGTGACC G^GT[ACGT]ACC
EcoT14I 244 CCTAGG C^C[AT][AT]GG
EcoT22I 1252 ATGCAT ATGCA^T
FauI 1205 GCGGG CCCGC(4/6)
FokI 117 GGATG GGATG(9/13)
HincII 351 GTTAAC GT[CT]^[GA]AC
HindII 351 GTTAAC GT[CT]^[GA]AC
HpaI 351 GTTAAC GTT^AAC
KpnI 310 GGTACC GGTAC^C
Ksp632I 289 GAAGAG CTCTTC(1/4)
MaeII 188 ACGT A^CGT
MslI 946 CACGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 259 CAGCTG C[AC]G^C[GT]G
MwoI 1254 GCATAATAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 1252 ATGCAT ATGCA^T
NspV 374 TTCGAA TT^CGAA
PvuII 259 CAGCTG CAG^CTG
SacI 76 GAGCTC GAGCT^C
Sau96I 159 GGACC G^G[ACGT]CC
SfaNI 192 GATGC GCATC(5/9)
SfuI 374 TTCGAA TT^CGAA
SmlI 741 CTTGAG C^T[CT][GA]AG
SspBI 179 TGTACA T^GTACA
StyI 244 CCTAGG C^C[AT][AT]GG
XbaI 388 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 299 GAATTC [GA]^AATT[CT]
746 GAATTC
ApoI 299 GAATTC [GA]^AATT[CT]
746 GAATTC
BfaI 245 CTAG C^TAG
389 CTAG
BglII 553 AGATCT A^GATCT
637 AGATCT
BseRI 142 GAGGAG GAGGAG(10/8)
969 GAGGAG
BsrDI 152 CATTGC GCAATG(2/0)
608 GCAATG
BssKI 138 CCAGG ^CC[ACGT]GG
213 CCTGG
BstNI 138 CCAGG CC^[AT]GG
213 CCTGG
BstYI 553 AGATCT [GA]^GATC[CT]
637 AGATCT
DraI 337 TTTAAA TTT^AAA
451 TTTAAA
EcoRI 299 GAATTC G^AATTC
746 GAATTC
EcoRII 138 CCAGG ^CC[AT]GG
213 CCTGG
HaeIII 123 GGCC GG^CC
216 GGCC
HphI 105 GGTGA GGTGA(8/7)
134 TCACC
MaeI 245 CTAG C^TAG
389 CTAG
MflI 553 AGATCT [GA]^GATC[CT]
637 AGATCT
MvaI 138 CCAGG CC^[AT]GG
213 CCTGG
NspI 366 GCATGT [GA]CATG^[CT]
1024 ACATGT
PstI 204 CTGCAG CTGCA^G
522 CTGCAG
ScrFI 138 CCAGG CC^[ACGT]GG
213 CCTGG
TaqI 375 TCGA T^CGA
976 TCGA
Tsp45I 106 GTGAC ^GT[GC]AC
695 GTGAC
TthHB8I 375 TCGA T^CGA
976 TCGA
XhoII 553 AGATCT [GA]^GATC[CT]
637 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI AluI Asp700I BbvI
BpmI BsaJI BsiYI BslI BsmI
BsrI DdeI DpnII Fnu4HI HinfI
ItaI MaeIII MboI MboII MnlI
MseI NdeII NlaIII NlaIV RsaI
Sau3AI SfcI TfiI Tru9I TseI
Tsp509I TspRI XmnI