(Circular) (Six-base) MAPSORT of: pFN21AB7725.seq Check: 5221 from: 1 to: 5974 pFN21AB7725 5974 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 2315 3295 2315 Size: 980 4994 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2449 2617 2701 2953 4037 5421 5689 1632 Size: 817 168 84 252 1084 1384 268 1917 Fragments arranged by size: 1917 1384 1084 817 268 252 168 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 3029 4873 5850 1203 Size: 1826 1844 977 1327 Fragments arranged by size: 1844 1826 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 2955 1166 Size: 1789 4185 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 2703 3717 5289 2703 Size: 1014 1572 3388 Fragments arranged by size: 3388 1572 1014 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 2304 2751 3283 3509 3661 5770 1493 Size: 811 447 532 226 152 2109 1697 Fragments arranged by size: 2109 1697 811 532 447 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 3299 1870 Size: 73 1356 4545 Fragments arranged by size: 4545 1356 73 AvrII C'CTAG_G Cuts at: 2249 2249 Size: 5974 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2315 3295 4750 618 Size: 325 217 627 528 980 1455 1842 Fragments arranged by size: 1842 1455 980 627 528 325 217 BanII G_rGCy'C Cuts at: 1391 2085 3293 1391 Size: 694 1208 4072 Fragments arranged by size: 4072 1208 694 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2455 928 Size: 971 556 4447 Fragments arranged by size: 4447 971 556 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2360 2394 4165 4199 1903 Size: 34 423 34 1771 34 3678 Fragments arranged by size: 3678 1771 423 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BfrBI ATG'CAT Cuts at: 3259 3259 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4585 136 Size: 107 122 71 631 605 337 2576 1525 Fragments arranged by size: 2576 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2558 2642 5969 2558 Size: 84 3327 2563 Fragments arranged by size: 3327 2563 84 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 4035 5191 1790 Size: 2245 1156 2573 Fragments arranged by size: 2573 2245 1156 BmgBI CAC'GTC Cuts at: 1318 2194 1318 Size: 876 5098 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2237 3705 4663 Size: 416 53 83 399 24 820 1468 958 Cuts at: 4663 442 Size: 1753 Fragments arranged by size: 1753 1468 958 820 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2324 2450 2916 2324 Size: 126 466 5382 Fragments arranged by size: 5382 466 126 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 2288 2529 2781 3033 4634 1239 Size: 983 66 241 252 252 1601 2579 Fragments arranged by size: 2579 1601 983 252 252 241 66 Bpu10I CC'TnA_GC Cuts at: 2364 2364 Size: 5974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2767 4099 4964 5262 2767 Size: 1332 865 298 3479 Fragments arranged by size: 3479 1332 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4637 882 Size: 617 3138 2219 Fragments arranged by size: 3138 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3765 493 Size: 711 2561 2702 Fragments arranged by size: 2702 2561 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4798 275 Size: 53 83 186 936 2248 382 635 1451 Fragments arranged by size: 2248 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2058 2088 1178 Size: 30 165 30 655 30 5064 Fragments arranged by size: 5064 655 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 2989 1186 Size: 976 827 4171 Fragments arranged by size: 4171 976 827 BseYI C'CCAG_C Cuts at: 2262 5177 5673 2262 Size: 2915 496 2563 Fragments arranged by size: 2915 2563 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3136 1163 Size: 573 1400 4001 Fragments arranged by size: 4001 1400 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 4035 4120 5191 2085 Size: 1950 85 1071 2868 Fragments arranged by size: 2868 1950 1071 85 BsmI GAATG_Cn' Cuts at: 2615 2699 2919 2951 3261 3485 3578 2615 Size: 84 220 32 310 224 93 5011 Fragments arranged by size: 5011 310 224 220 93 84 32 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 3293 4035 4120 5191 1391 Size: 399 295 1208 742 85 1071 2174 Fragments arranged by size: 2174 1208 1071 742 399 295 85 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3329 844 Size: 2485 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3861 1394 Size: 429 2038 3507 Fragments arranged by size: 3507 2038 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2156 2620 4469 4643 65 Size: 1183 908 464 1849 174 1396 Fragments arranged by size: 1849 1396 1183 908 464 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4618 4805 1700 Size: 110 2808 187 2869 Fragments arranged by size: 2869 2808 187 110 BsrGI T'GTAC_A Cuts at: 96 2184 96 Size: 2088 3886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 2890 4034 5046 2890 Size: 1144 1012 3818 Fragments arranged by size: 3818 1144 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 2380 4778 2380 Size: 2398 3576 BstEII G'GTnAC_C Cuts at: 1184 2110 1184 Size: 926 5048 BstXI CCAn_nnnn'nTGG Cuts at: 2957 2957 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2558 2642 3304 3671 4056 4073 5525 5611 Size: 586 84 662 367 385 17 1452 86 Cuts at: 5611 5623 5969 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 662 586 385 367 346 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2130 513 Size: 552 939 126 4357 Fragments arranged by size: 4357 939 552 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 2536 2704 3124 3490 4284 4304 2536 Size: 168 420 366 794 20 4206 Fragments arranged by size: 4206 794 420 366 168 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2602 2637 529 Size: 35 2038 35 3866 Fragments arranged by size: 3866 2038 35 35 DraI TTT'AAA Cuts at: 2344 2458 3278 2344 Size: 114 820 5040 Fragments arranged by size: 5040 820 114 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 3421 4310 Size: 54 1425 50 275 18 297 1294 889 Cuts at: 4310 5939 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1294 889 297 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2289 3904 1353 Size: 936 1615 3423 Fragments arranged by size: 3423 1615 936 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2079 4561 4935 5081 387 Size: 1064 138 490 2482 374 146 1280 Fragments arranged by size: 2482 1280 1064 490 374 146 138 EcoICRI GAG'CTC Cuts at: 2083 2083 Size: 5974 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2288 2449 2529 2617 2701 2781 Size: 393 590 66 161 80 88 84 80 Cuts at: 2781 2953 3033 4037 4634 5421 5689 1239 Size: 172 80 1004 597 787 268 1524 Fragments arranged by size: 1524 1004 787 597 590 393 268 172 161 88 84 80 80 80 66 EcoNI CCTnn'n_nnAGG Cuts at: 2971 2971 Size: 5974 EcoRI G'AATT_C Cuts at: 2304 2751 3283 3661 2304 Size: 447 532 378 4617 Fragments arranged by size: 4617 532 447 378 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 5121 1678 Size: 3443 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2404 2436 2968 3000 4615 Size: 32 135 32 832 32 532 32 1615 Cuts at: 4615 4647 4689 4721 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1615 832 532 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2358 3318 3564 669 Size: 988 281 420 960 246 3079 Fragments arranged by size: 3079 988 960 420 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 2358 3564 2358 Size: 1206 4768 KpnI G_GTAC'C Cuts at: 2319 3299 2319 Size: 980 4994 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5088 5272 1305 Size: 3783 184 2007 Fragments arranged by size: 3783 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2955 3932 4291 4450 518 Size: 648 1789 977 359 159 2042 Fragments arranged by size: 2042 1789 977 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2266 3390 4067 5215 5460 1606 Size: 660 1124 677 1148 245 2120 Fragments arranged by size: 2120 1148 1124 677 660 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4483 Fragments arranged by size: 4483 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NsiI A_TGCA'T Cuts at: 3261 3261 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 2375 3033 3332 4877 1132 Size: 97 1146 658 299 1545 2229 Fragments arranged by size: 2229 1545 1146 658 299 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 3029 4873 3029 Size: 1844 4130 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5974 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2207 2239 4043 4075 5556 5588 2207 Size: 32 1804 32 1481 32 2593 Fragments arranged by size: 2593 1804 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PstI C_TGCA'G Cuts at: 830 2213 2531 3326 830 Size: 1383 318 795 3478 Fragments arranged by size: 3478 1383 795 318 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2266 2266 Size: 5974 SacI G_AGCT'C Cuts at: 2085 2085 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 2209 2527 2548 2914 3322 4457 5138 Size: 219 1164 318 21 366 408 1135 681 Cuts at: 5138 5329 826 Size: 191 1471 Fragments arranged by size: 1471 1164 1135 681 408 366 318 219 191 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2746 4078 4979 5241 820 Size: 197 926 803 1332 901 262 1553 Fragments arranged by size: 1553 1332 926 901 803 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 2004 2249 3413 513 Size: 552 939 245 1164 3074 Fragments arranged by size: 3074 1164 939 552 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2156 4001 4018 4171 4356 4801 325 Size: 1255 576 1845 17 153 185 445 1498 Fragments arranged by size: 1845 1498 1255 576 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 4220 5932 Size: 274 80 33 51 1443 207 2036 1712 Cuts at: 5932 96 Size: 138 Fragments arranged by size: 2036 1712 1443 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5934 5966 5934 Size: 32 5942 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 2393 3310 2393 Size: 917 5057 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 2630 2714 3218 4103 2630 Size: 84 504 885 4501 Fragments arranged by size: 4501 885 504 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI