Restriction map for
pFN21AB7725
(Linear) (Six-base) MAPSORT of: KIBB7725.seq Check: 2968 from: 1 to: 1269
KIBB7725.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
Acc65I G'GTAC_C
Cuts at: 0 310 1269
Size: 310 959
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 612 696 948 1269
Size: 444 168 84 252 321
Fragments arranged by size:
444 321 252 168 84
AflIII A'CryG_T
Cuts at: 0 1024 1269
Size: 1024 245
AleI CACnn'nnGTG
Cuts at: 0 950 1269
Size: 950 319
AlwNI CAG_nnn'CTG
Cuts at: 0 698 1269
Size: 698 571
ApoI r'AATT_y
Cuts at: 0 299 746 1269
Size: 299 447 523
Fragments arranged by size:
523 447 299
AvrII C'CTAG_G
Cuts at: 0 244 1269
Size: 244 1025
BanI G'GyrC_C
Cuts at: 0 310 1269
Size: 310 959
BanII G_rGCy'C
Cuts at: 0 80 1269
Size: 80 1189
BbsI GAAGACnn'nnnn_
Cuts at: 0 450 1269
Size: 450 819
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 355 389 1269
Size: 355 34 880
Fragments arranged by size:
880 355 34
BfrBI ATG'CAT
Cuts at: 0 1254 1269
Size: 1254 15
BglII A'GATC_T
Cuts at: 0 553 637 1269
Size: 553 84 632
Fragments arranged by size:
632 553 84
BmgBI CAC'GTC
Cuts at: 0 189 1269
Size: 189 1080
BmrI ACTGGGnnnn_n'
Cuts at: 0 232 1269
Size: 232 1037
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 319 445 911 1269
Size: 319 126 466 358
Fragments arranged by size:
466 358 319 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 283 524 776 1028 1269
Size: 217 66 241 252 252 241
Fragments arranged by size:
252 252 241 241 217 66
Bpu10I CC'TnA_GC
Cuts at: 0 359 1269
Size: 359 910
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 1269
Size: 762 507
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 53 83 1269
Size: 53 30 1186
Fragments arranged by size:
1186 53 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 984 1269
Size: 157 827 285
Fragments arranged by size:
827 285 157
BseYI C'CCAG_C
Cuts at: 0 257 1269
Size: 257 1012
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1131 1269
Size: 1131 138
BsiHKAI G_wGCw'C
Cuts at: 0 80 1269
Size: 80 1189
BsmI GAATG_Cn'
Cuts at: 0 610 694 914 946 1256 1269
Size: 610 84 220 32 310 13
Fragments arranged by size:
610 310 220 84 32 13
Bsp1286I G_dGCh'C
Cuts at: 0 80 1269
Size: 80 1189
BsrDI GCAATG_nn'
Cuts at: 0 151 615 1269
Size: 151 464 654
Fragments arranged by size:
654 464 151
BsrGI T'GTAC_A
Cuts at: 0 179 1269
Size: 179 1090
BssSI C'ACGA_G
Cuts at: 0 885 1269
Size: 885 384
BstBI TT'CG_AA
Cuts at: 0 375 1269
Size: 375 894
BstEII G'GTnAC_C
Cuts at: 0 105 1269
Size: 105 1164
BstXI CCAn_nnnn'nTGG
Cuts at: 0 952 1269
Size: 952 317
BstYI r'GATC_y
Cuts at: 0 553 637 1269
Size: 553 84 632
Fragments arranged by size:
632 553 84
BtgI C'CryG_G
Cuts at: 0 125 1269
Size: 125 1144
BtsI GCAGTG_nn'
Cuts at: 0 531 699 1119 1269
Size: 531 168 420 150
Fragments arranged by size:
531 420 168 150
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 597 632 1269
Size: 597 35 637
Fragments arranged by size:
637 597 35
DraI TTT'AAA
Cuts at: 0 339 453 1269
Size: 339 114 816
Fragments arranged by size:
816 339 114
EaeI y'GGCC_r
Cuts at: 0 122 1269
Size: 122 1147
EarI CTCTTCn'nnn_
Cuts at: 0 284 1269
Size: 284 985
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 74 1269
Size: 74 1195
EcoICRI GAG'CTC
Cuts at: 0 78 1269
Size: 78 1191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 283 444 524 612 696 776 948
Size: 217 66 161 80 88 84 80 172
Cuts at: 948 1028 1269
Size: 80 241
Fragments arranged by size:
241 217 172 161 88 84 80 80
80 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 966 1269
Size: 966 303
EcoRI G'AATT_C
Cuts at: 0 299 746 1269
Size: 299 447 523
Fragments arranged by size:
523 447 299
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 399 431 963 995 1269
Size: 399 32 532 32 274
Fragments arranged by size:
532 399 274 32 32
HincII GTy'rAC
Cuts at: 0 353 1269
Size: 353 916
HpaI GTT'AAC
Cuts at: 0 353 1269
Size: 353 916
KpnI G_GTAC'C
Cuts at: 0 314 1269
Size: 314 955
MslI CAynn'nnrTG
Cuts at: 0 950 1269
Size: 950 319
MspA1I CmG'CkG
Cuts at: 0 261 1269
Size: 261 1008
NsiI A_TGCA'T
Cuts at: 0 1256 1269
Size: 1256 13
NspI r_CATG'y
Cuts at: 0 370 1028 1269
Size: 370 658 241
Fragments arranged by size:
658 370 241
PciI A'CATG_T
Cuts at: 0 1024 1269
Size: 1024 245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 1269
Size: 202 32 1035
Fragments arranged by size:
1035 202 32
PstI C_TGCA'G
Cuts at: 0 208 526 1269
Size: 208 318 743
Fragments arranged by size:
743 318 208
PvuII CAG'CTG
Cuts at: 0 261 1269
Size: 261 1008
SacI G_AGCT'C
Cuts at: 0 80 1269
Size: 80 1189
SfcI C'TryA_G
Cuts at: 0 204 522 543 909 1269
Size: 204 318 21 366 360
Fragments arranged by size:
366 360 318 204 21
SmlI C'TyrA_G
Cuts at: 0 741 1269
Size: 741 528
StyI C'CwwG_G
Cuts at: 0 244 1269
Size: 244 1025
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 151 1269
Size: 151 1118
TatI w'GTAC_w
Cuts at: 0 179 1269
Size: 179 1090
XbaI T'CTAG_A
Cuts at: 0 388 1269
Size: 388 881
XmnI GAAnn'nnTTC
Cuts at: 0 625 709 1213 1269
Size: 625 84 504 56
Fragments arranged by size:
625 504 84 56
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlwNI ApoI AvrII BanI
BanII BbsI BcgI BfrBI BglII BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BsrDI BsrGI BssSI BstBI BstEII BstXI
BstYI BtgI BtsI CspCI DraI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI Hin4I HincII HpaI KpnI
MslI MspA1I NsiI NspI PciI PpiI PstI PvuII
SacI SfcI SmlI StyI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
BaeI BamHI BbeI BbvCI BciVI BclI BglI BlpI
Bme1580I BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII
BstAPI BstZ17I Bsu36I BtgZI ClaI DraIII DrdI EagI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HindIII
KasI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XcmI XhoI XmaI ZraI