(Circular) (Six-base) MAPSORT of: pFN21AB6516.seq Check: 7567 from: 1 to: 5860 pFN21AB6516 5860 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2588 2588 Size: 5860 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3203 1937 Size: 1266 4594 Acc65I G'GTAC_C Cuts at: 2660 3181 2660 Size: 521 5339 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2388 2677 2683 2701 2748 2863 3923 5307 Size: 756 289 6 18 47 115 1060 1384 Cuts at: 5307 5575 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1060 756 289 268 115 47 18 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 4759 5736 1203 Size: 3556 977 1327 Fragments arranged by size: 3556 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AlwNI CAG_nnn'CTG Cuts at: 3603 5175 3603 Size: 1572 4288 ApaI G_GGCC'C Cuts at: 2610 2610 Size: 5860 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 3169 3395 3547 5656 1493 Size: 1676 226 152 2109 1697 Fragments arranged by size: 2109 1697 1676 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 3185 1870 Size: 73 1242 4545 Fragments arranged by size: 4545 1242 73 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2660 3181 4636 618 Size: 325 217 627 873 521 1455 1842 Fragments arranged by size: 1842 1455 873 627 521 325 217 BanII G_rGCy'C Cuts at: 1391 2400 2610 3179 1391 Size: 1009 210 569 4072 Fragments arranged by size: 4072 1009 569 210 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2282 928 Size: 971 383 4506 Fragments arranged by size: 4506 971 383 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4051 4085 1903 Size: 34 2114 34 3678 Fragments arranged by size: 3678 2114 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4471 136 Size: 107 122 71 631 605 2799 1525 Fragments arranged by size: 2799 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2433 5855 2433 Size: 3422 2438 BlpI GC'TnA_GC Cuts at: 3277 3277 Size: 5860 Bme1580I G_kGCm'C Cuts at: 1790 2610 3921 5077 1790 Size: 820 1311 1156 2573 Fragments arranged by size: 2573 1311 1156 820 BmgBI CAC'GTC Cuts at: 1318 2340 1318 Size: 1022 4838 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2392 2605 2754 Size: 416 53 83 399 24 975 213 149 Cuts at: 2754 3591 4549 442 Size: 837 958 1753 Fragments arranged by size: 1753 975 958 837 416 399 213 149 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 2775 4520 1239 Size: 1052 484 1745 2579 Fragments arranged by size: 2579 1745 1052 484 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3985 4850 5148 3985 Size: 865 298 4697 Fragments arranged by size: 4697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4523 882 Size: 617 3024 2219 Fragments arranged by size: 3024 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3651 493 Size: 711 2447 2702 Fragments arranged by size: 2702 2447 711 BsaBI GATnn'nnATC Cuts at: 2312 2438 5854 2312 Size: 126 3416 2318 Fragments arranged by size: 3416 2318 126 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2478 3667 4049 4684 Size: 53 83 186 936 945 1189 382 635 Cuts at: 4684 275 Size: 1451 Fragments arranged by size: 1451 1189 945 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2316 4293 4691 4965 5112 5523 2316 Size: 1977 398 274 147 411 2653 Fragments arranged by size: 2653 1977 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2541 2571 1178 Size: 30 165 30 1138 30 4467 Fragments arranged by size: 4467 1138 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5860 BseYI C'CCAG_C Cuts at: 2656 5063 5559 2656 Size: 2407 496 2957 Fragments arranged by size: 2957 2407 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5287 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2072 3921 4006 5077 2072 Size: 1849 85 1071 2855 Fragments arranged by size: 2855 1849 1071 85 BsmI GAATG_Cn' Cuts at: 3074 3371 3464 3074 Size: 297 93 5470 Fragments arranged by size: 5470 297 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2400 2610 3179 3921 4006 5077 Size: 399 282 328 210 569 742 85 1071 Cuts at: 5077 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 569 399 328 282 210 85 BspEI T'CCGG_A Cuts at: 2316 5523 2316 Size: 3207 2653 BspHI T'CATG_A Cuts at: 2850 3749 5479 2850 Size: 899 1730 3231 Fragments arranged by size: 3231 1730 899 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2588 3215 844 Size: 1744 627 3489 Fragments arranged by size: 3489 1744 627 BsrBI CCG'CTC Cuts at: 1394 1823 3747 1394 Size: 429 1924 3507 Fragments arranged by size: 3507 1924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4355 4529 65 Size: 1183 3107 174 1396 Fragments arranged by size: 3107 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4504 4691 1700 Size: 110 2694 187 2869 Fragments arranged by size: 2869 2694 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5860 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5860 BstYI r'GATC_y Cuts at: 1972 2433 3190 3557 3942 3959 5411 5497 5509 Size: 461 757 367 385 17 1452 86 12 Cuts at: 5509 5855 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 757 461 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2054 2545 2664 513 Size: 552 939 50 491 119 3709 Fragments arranged by size: 3709 939 552 491 119 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5121 Fragments arranged by size: 5121 683 56 BtsI GCAGTG_nn' Cuts at: 2545 3376 4170 4190 2545 Size: 831 794 20 4215 Fragments arranged by size: 4215 831 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DraIII CAC_nnn'GTG Cuts at: 2711 2711 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2542 2871 3307 Size: 54 1425 50 275 18 712 329 436 Cuts at: 3307 4196 5825 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 712 436 329 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3050 3790 1353 Size: 1697 740 3423 Fragments arranged by size: 3423 1697 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2270 4447 4821 4967 387 Size: 1064 138 681 2177 374 146 1280 Fragments arranged by size: 2177 1280 1064 681 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2388 2677 2683 2701 2748 2775 Size: 393 659 97 289 6 18 47 27 Cuts at: 2775 2863 3923 4520 5307 5575 1239 Size: 88 1060 597 787 268 1524 Fragments arranged by size: 1524 1060 787 659 597 393 289 268 97 88 47 27 18 6 EcoO109I rG'GnC_Cy Cuts at: 2607 2647 2607 Size: 40 5820 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 5007 1678 Size: 3329 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2813 2845 4501 4533 4575 Size: 32 135 32 1241 32 1656 32 42 Cuts at: 4575 4607 1373 Size: 32 2626 Fragments arranged by size: 2626 1656 1241 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 2664 3185 2664 Size: 521 5339 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4974 5158 1305 Size: 3669 184 2007 Fragments arranged by size: 3669 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2544 2873 10 Size: 54 1425 343 712 329 2997 Fragments arranged by size: 2997 1425 712 343 329 54 MslI CAynn'nnrTG Cuts at: 518 1166 2059 3053 3818 4177 4336 518 Size: 648 893 994 765 359 159 2042 Fragments arranged by size: 2042 994 893 765 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2721 3276 3953 5101 5346 1606 Size: 1115 555 677 1148 245 2120 Fragments arranged by size: 2120 1148 1115 677 555 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2054 2545 2664 513 Size: 552 939 50 491 119 3709 Fragments arranged by size: 3709 939 552 491 119 50 NdeI CA'TA_TG Cuts at: 387 3157 387 Size: 2770 3090 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 2898 3218 4763 1132 Size: 97 1669 320 1545 2229 Fragments arranged by size: 2229 1669 1545 320 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 2190 2869 1108 Size: 1082 679 4099 Fragments arranged by size: 4099 1082 679 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3036 3430 3036 Size: 394 5466 PspOMI G'GGCC_C Cuts at: 2606 2606 Size: 5860 PstI C_TGCA'G Cuts at: 830 2015 3212 830 Size: 1185 1197 3478 Fragments arranged by size: 3478 1197 1185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2721 2721 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2011 2857 3208 4343 5024 5215 826 Size: 219 966 846 351 1135 681 191 1471 Fragments arranged by size: 1471 1135 966 846 681 351 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3964 4865 5127 820 Size: 197 926 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 2004 2054 2545 2664 3299 513 Size: 552 939 50 491 119 635 3074 Fragments arranged by size: 3074 939 635 552 491 119 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2087 2332 3887 3904 4057 4242 4687 Size: 1255 507 245 1555 17 153 185 445 Cuts at: 4687 325 Size: 1498 Fragments arranged by size: 1555 1498 1255 507 445 245 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2672 4106 5818 Size: 274 80 33 51 1443 695 1434 1712 Cuts at: 5818 96 Size: 138 Fragments arranged by size: 1712 1443 1434 695 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5820 5852 5820 Size: 32 5828 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2778 2778 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 2093 3989 2093 Size: 1896 3964 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI