Restriction map for
pFN21AE4040
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbsI
BbvCI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AfaI 558 GTAC GT^AC
AflIII 554 ACGTGT A^C[GA][CT]GT
AlwNI 157 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 266 CCTTAGG CC^T[ACGT]AGG
ApaI 88 GGGCCC GGGCC^C
AspHI 586 GAGCTC G[AT]GC[AT]^C
AvaI 184 CCCGAG C^[CT]CG[GA]G
BanI 238 GGCACC G^G[CT][GA]CC
BbrPI 553 CACGTG CAC^GTG
BcgI 239 GCACCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 509 GGATAC GTATCC(6/5)
BsaAI 553 CACGTG [CT]AC^GT[GA]
BsiEI 132 CGGCCG CG[GA][CT]^CG
BsiHKAI 586 GAGCTC G[AT]GC[AT]^C
BsoBI 184 CCCGAG C^[CT]CG[GA]G
Bsp1407I 557 TGTACA T^GTACA
BsrBI 187 GAGCGG CCGCTC(-3/-3)
BsrDI 668 GCAATG GCAATG(2/0)
BsrGI 557 TGTACA T^GTACA
BsrI 79 CCAGT ACTGG(1/-1)
BstBI 628 TTCGAA TT^CGAA
BstNI 309 CCAGG CC^[AT]GG
BstYI 649 GGATCT [GA]^GATC[CT]
Bsu36I 266 CCTTAGG CC^T[ACGT]AGG
EaeI 132 CGGCCG [CT]^GGCC[GA]
EagI 132 CGGCCG C^GGCCG
EarI 583 GAAGAG CTCTTC(1/4)
EclXI 132 CGGCCG C^GGCCG
Eco52I 132 CGGCCG C^GGCCG
Eco81I 266 CCTTAGG CC^T[ACGT]AGG
EcoRI 685 GAATTC G^AATTC
EcoRII 309 CCAGG ^CC[AT]GG
HgaI 481 GCGTC GACGC(5/10)
Ksp632I 583 GAAGAG CTCTTC(1/4)
MaeIII 250 GTGAC ^GT[ACGT]AC
MflI 649 GGATCT [GA]^GATC[CT]
MseI 500 TTAA T^TAA
MslI 591 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 309 CCAGG CC^[AT]GG
NspV 628 TTCGAA TT^CGAA
PmaCI 553 CACGTG CAC^GTG
PmlI 553 CACGTG CAC^GTG
PvuII 13 CAGCTG CAG^CTG
RsaI 558 GTAC GT^AC
SacI 586 GAGCTC GAGCT^C
SapI 583 GAAGAGC GCTCTTC(1/4)
SfuI 628 TTCGAA TT^CGAA
SspBI 557 TGTACA T^GTACA
TaqI 629 TCGA T^CGA
TfiI 762 GATTC G^A[AT]TC
Tru9I 500 TTAA T^TAA
Tsp45I 250 GTGAC ^GT[GC]AC
TspRI 571 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 629 TCGA T^CGA
XhoII 649 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 625 AAATTC [GA]^AATT[CT]
685 GAATTC
ApoI 625 AAATTC [GA]^AATT[CT]
685 GAATTC
BglI 104 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
120 GCCCCGCGGGC
BsmAI 343 GAGAC GTCTC(1/5)
700 GTCTC
BstF5I 339 GGATG GGATG(2/0)
785 GGATG
CfoI 154 GCGC GCG^C
206 GCGC
FokI 339 GGATG GGATG(9/13)
785 GGATG
HhaI 154 GCGC GCG^C
206 GCGC
HinP1I 154 GCGC G^CGC
206 GCGC
KspI 84 CCGCGG CCGC^GG
123 CCGCGG
MaeII 230 ACGT A^CGT
554 ACGT
PleI 328 GACTC GAGTC(4/5)
563 GAGTC
PstI 10 CTGCAG CTGCA^G
568 CTGCAG
SacII 84 CCGCGG CCGC^GG
123 CCGCGG
SfaNI 644 GCATC GCATC(5/9)
731 GCATC
SfcI 10 CTGCAG C^T[GA][CT]AG
568 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstUI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I FauI
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MboI MboII MnlI MspA1I
MspI MvnI MwoI NciI NdeII
NlaIV PpuMI Sau3AI Sau96I ScrFI
TseI Tsp509I