(Circular) (Six-base) MAPSORT of: pFN21AE4040.seq Check: 2398 from: 1 to: 5494 pFN21AE4040 5494 bp With 182 enzymes: SgfI * March 28, 2012 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3304 278 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2837 1937 Size: 900 4594 Acc65I G'GTAC_C Cuts at: 2815 2815 Size: 5494 AclI AA'CG_TT Cuts at: 1563 3621 3994 1563 Size: 2058 373 3063 Fragments arranged by size: 3063 2058 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2756 3557 4941 5209 1632 Size: 1124 801 1384 268 1917 Fragments arranged by size: 1917 1384 1124 801 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5297 AflIII A'CryG_T Cuts at: 1203 2559 4393 5370 1203 Size: 1356 1834 977 1327 Fragments arranged by size: 1834 1356 1327 977 AgeI A'CCGG_T Cuts at: 4325 4325 Size: 5494 AhdI GACnn_n'nnGTC Cuts at: 4223 4223 Size: 5494 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5494 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5494 AlwNI CAG_nnn'CTG Cuts at: 2167 3237 4809 2167 Size: 1070 1572 2852 Fragments arranged by size: 2852 1572 1070 ApaI G_GGCC'C Cuts at: 2097 2097 Size: 5494 ApaLI G'TGCA_C Cuts at: 3551 4707 3551 Size: 1156 4338 ApoI r'AATT_y Cuts at: 1493 2630 2690 2803 3029 3181 5290 1493 Size: 1137 60 113 226 152 2109 1697 Fragments arranged by size: 2109 1697 1137 226 152 113 60 AseI AT'TA_AT Cuts at: 160 1032 4048 160 Size: 872 3016 1606 Fragments arranged by size: 3016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 AvaI C'yCGr_G Cuts at: 1870 1943 2189 2819 1870 Size: 73 246 630 4545 Fragments arranged by size: 4545 630 246 73 BamHI G'GATC_C Cuts at: 2824 3191 2824 Size: 367 5127 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2243 2815 4270 618 Size: 325 217 627 456 572 1455 1842 Fragments arranged by size: 1842 1455 627 572 456 325 217 BanII G_rGCy'C Cuts at: 1391 2097 2286 2595 2813 1391 Size: 706 189 309 218 4072 Fragments arranged by size: 4072 706 309 218 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4523 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2233 2267 3685 3719 1903 Size: 34 296 34 1418 34 3678 Fragments arranged by size: 3678 1418 296 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2508 3386 4596 2508 Size: 878 1210 3406 Fragments arranged by size: 3406 1210 878 BclI T'GATC_A Cuts at: 1548 1548 Size: 5494 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2115 2131 4105 Size: 107 122 71 631 605 443 16 1974 Cuts at: 4105 136 Size: 1525 Fragments arranged by size: 1974 1525 631 605 443 122 107 71 16 BglII A'GATC_T Cuts at: 5489 5489 Size: 5494 BlpI GC'TnA_GC Cuts at: 2911 2911 Size: 5494 Bme1580I G_kGCm'C Cuts at: 1790 2097 2136 3555 4711 1790 Size: 307 39 1419 1156 2573 Fragments arranged by size: 2573 1419 1156 307 39 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5494 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2078 3225 4183 Size: 416 53 83 399 24 661 1147 958 Cuts at: 4183 442 Size: 1753 Fragments arranged by size: 1753 1147 958 661 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5494 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2350 2350 Size: 5494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2480 2692 4154 1239 Size: 950 291 212 1462 2579 Fragments arranged by size: 2579 1462 950 291 212 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3619 4484 4782 3619 Size: 865 298 4331 Fragments arranged by size: 4331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4157 882 Size: 617 2658 2219 Fragments arranged by size: 2658 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2560 3285 493 Size: 711 1356 725 2702 Fragments arranged by size: 2702 1356 725 711 BsaBI GATnn'nnATC Cuts at: 5488 5488 Size: 5494 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3301 3683 4318 275 Size: 53 83 186 936 1768 382 635 1451 Fragments arranged by size: 1768 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3927 4325 4599 4746 5157 3927 Size: 398 274 147 411 4264 Fragments arranged by size: 4264 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2273 2303 1178 Size: 30 165 30 870 30 4369 Fragments arranged by size: 4369 870 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5494 BseYI C'CCAG_C Cuts at: 2041 2099 2220 4697 5193 2041 Size: 58 121 2477 496 2342 Fragments arranged by size: 2477 2342 496 121 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4921 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2140 2944 3705 4733 1540 Size: 275 186 139 804 761 1028 2301 Fragments arranged by size: 2301 1028 804 761 275 186 139 BsiHKAI G_wGCw'C Cuts at: 2595 3555 3640 4711 2595 Size: 960 85 1071 3378 Fragments arranged by size: 3378 1071 960 85 BsmI GAATG_Cn' Cuts at: 3005 3098 3005 Size: 93 5401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2136 2286 2595 2813 3555 3640 Size: 399 307 39 150 309 218 742 85 Cuts at: 3640 4711 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 309 307 218 150 85 39 BspEI T'CCGG_A Cuts at: 5157 5157 Size: 5494 BspHI T'CATG_A Cuts at: 3383 5113 3383 Size: 1730 3764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2849 844 Size: 2005 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2194 3381 1394 Size: 429 371 1187 3507 Fragments arranged by size: 3507 1187 429 371 BsrDI GCAATG_nn' Cuts at: 65 1248 2680 3989 4163 65 Size: 1183 1432 1309 174 1396 Fragments arranged by size: 1432 1396 1309 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4138 4325 1700 Size: 110 2328 187 2869 Fragments arranged by size: 2869 2328 187 110 BsrGI T'GTAC_A Cuts at: 96 2562 96 Size: 2466 3028 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5494 BssSI C'ACGA_G Cuts at: 3554 4566 3554 Size: 1012 4482 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5494 BstBI TT'CG_AA Cuts at: 2634 4298 2634 Size: 1664 3830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5494 BstYI r'GATC_y Cuts at: 1972 2654 2824 3191 3576 3593 5045 5131 5143 Size: 682 170 367 385 17 1452 86 12 Cuts at: 5143 5489 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 682 385 367 346 170 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2272 2272 Size: 5494 BtgI C'CryG_G Cuts at: 513 1065 2004 2089 2128 2413 513 Size: 552 939 85 39 285 3594 Fragments arranged by size: 3594 939 552 285 85 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2045 2467 487 Size: 683 56 819 422 3514 Fragments arranged by size: 3514 819 683 422 56 BtsI GCAGTG_nn' Cuts at: 2582 3010 3804 3824 2582 Size: 428 794 20 4252 Fragments arranged by size: 4252 794 428 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5494 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2368 2403 529 Size: 35 1804 35 3620 Fragments arranged by size: 3620 1804 35 35 DraI TTT'AAA Cuts at: 2798 2798 Size: 5494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4501 809 Size: 507 3185 1802 Fragments arranged by size: 3185 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 2941 3830 Size: 54 1425 50 275 18 307 804 889 Cuts at: 3830 5459 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 804 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2137 2941 1537 Size: 275 325 804 4090 Fragments arranged by size: 4090 804 325 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2583 3424 1353 Size: 1230 841 3423 Fragments arranged by size: 3423 1230 841 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2399 4081 4455 4601 387 Size: 1064 138 435 375 1682 374 146 1280 Fragments arranged by size: 1682 1280 1064 435 375 374 146 138 EcoICRI GAG'CTC Cuts at: 2593 2593 Size: 5494 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2480 2692 2756 3557 4154 4941 Size: 393 557 291 212 64 801 597 787 Cuts at: 4941 5209 1239 Size: 268 1524 Fragments arranged by size: 1524 801 787 597 557 393 291 268 212 64 EcoO109I rG'GnC_Cy Cuts at: 2094 2177 2320 2374 2546 2094 Size: 83 143 54 172 5042 Fragments arranged by size: 5042 172 143 83 54 EcoRI G'AATT_C Cuts at: 2690 2803 3181 2690 Size: 113 378 5003 Fragments arranged by size: 5003 378 113 FspI TGC'GCA Cuts at: 4000 4000 Size: 5494 HaeII r_GCGC'y Cuts at: 1678 4641 1678 Size: 2963 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2273 2305 2768 2800 4135 Size: 32 135 32 701 32 463 32 1335 Cuts at: 4135 4167 4209 4241 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1335 701 463 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2838 3084 669 Size: 988 281 900 246 3079 Fragments arranged by size: 3079 988 900 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5494 HpaI GTT'AAC Cuts at: 3084 3084 Size: 5494 KpnI G_GTAC'C Cuts at: 2819 2819 Size: 5494 MfeI C'AATT_G Cuts at: 3093 3093 Size: 5494 MluI A'CGCG_T Cuts at: 5370 5370 Size: 5494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2643 4608 4792 1305 Size: 1338 1965 184 2007 Fragments arranged by size: 2007 1965 1338 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3672 Fragments arranged by size: 3672 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2600 3452 3811 3970 518 Size: 648 1434 852 359 159 2042 Fragments arranged by size: 2042 1434 852 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2020 2091 2130 2167 2220 2910 3587 4735 Size: 414 71 39 37 53 690 677 1148 Cuts at: 4735 4980 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 690 677 414 245 71 53 39 37 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4003 Fragments arranged by size: 4003 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5494 NotI GC'GGCC_GC Cuts at: 2941 2941 Size: 5494 NspI r_CATG'y Cuts at: 1132 1229 2852 4397 1132 Size: 97 1623 1545 2229 Fragments arranged by size: 2229 1623 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5494 PciI A'CATG_T Cuts at: 4393 4393 Size: 5494 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5494 PfoI T'CCnGG_A Cuts at: 2179 2179 Size: 5494 PmeI GTTT'AAAC Cuts at: 2798 2798 Size: 5494 PmlI CAC'GTG Cuts at: 2560 2560 Size: 5494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 5076 5108 3563 Size: 32 1481 32 3949 Fragments arranged by size: 3949 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2177 2320 2374 2177 Size: 143 54 5297 Fragments arranged by size: 5297 143 54 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5494 PsiI TTA'TAA Cuts at: 3064 3064 Size: 5494 PspOMI G'GGCC_C Cuts at: 2093 2093 Size: 5494 PstI C_TGCA'G Cuts at: 830 2019 2577 2846 830 Size: 1189 558 269 3478 Fragments arranged by size: 3478 1189 558 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5494 PvuII CAG'CTG Cuts at: 2020 2020 Size: 5494 SacI G_AGCT'C Cuts at: 2595 2595 Size: 5494 SacII CC_GC'GG Cuts at: 2092 2131 2092 Size: 39 5455 SalI G'TCGA_C Cuts at: 1936 2836 1936 Size: 900 4594 SanDI GG'GwC_CC Cuts at: 2177 2374 2177 Size: 197 5297 SapI GCTCTTCn'nnn_ Cuts at: 2583 2583 Size: 5494 SbfI CC_TGCA'GG Cuts at: 2846 2846 Size: 5494 ScaI AGT'ACT Cuts at: 3742 5454 3742 Size: 1712 3782 SfcI C'TryA_G Cuts at: 826 1045 2015 2573 2842 3977 4658 4849 826 Size: 219 970 558 269 1135 681 191 1471 Fragments arranged by size: 1471 1135 970 681 558 269 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5494 SmaI CCC'GGG Cuts at: 1872 2821 1872 Size: 949 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3598 4499 4761 820 Size: 197 926 1655 901 262 1553 Fragments arranged by size: 1655 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3285 493 Size: 2792 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5494 SphI G_CATG'C Cuts at: 1132 2852 1132 Size: 1720 3774 SspI AAT'ATT Cuts at: 5 52 3418 5 Size: 47 3366 2081 Fragments arranged by size: 3366 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5494 StyI C'CwwG_G Cuts at: 513 1065 2004 2933 513 Size: 552 939 929 3074 Fragments arranged by size: 3074 939 929 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3521 3538 3691 3876 4321 325 Size: 1255 1941 17 153 185 445 1498 Fragments arranged by size: 1941 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2562 3740 5452 Size: 274 80 33 51 1443 585 1178 1712 Cuts at: 5452 96 Size: 138 Fragments arranged by size: 1712 1443 1178 585 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5454 5486 5454 Size: 32 5462 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5494 XbaI T'CTAG_A Cuts at: 2830 2830 Size: 5494 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5494 XmaI C'CCGG_G Cuts at: 1870 2819 1870 Size: 949 4545 XmnI GAAnn'nnTTC Cuts at: 3623 3623 Size: 5494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3302 276 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SexAI SfiI SfoI SrfI SwaI XcmI