################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII BalI BamHI BanI BbeI BbrPI BciVI BfrI BglI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmFI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MluI MluNI MroI MscI MslI NaeI NarI NcoI NgoMIV NheI NotI NruI NspI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 2090 CGCG CG^CG AspHI 453 GAGCTC G[AT]GC[AT]^C AvaI 839 CTCGAG C^[CT]CG[GA]G AvaII 169 GGACC G^G[AT]CC AvrII 310 CCTAGG C^CTAGG BbsI 1908 GTCTTC GAAGAC(2/6) BbvCI 699 GCTGAGG CCTCAGC(-5/-2) BcgI 1119 CGAGTTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 404 CCGGG CC^[GC]GG BlnI 310 CCTAGG C^CTAGG BpuAI 1908 GTCTTC GAAGAC(2/6) BsaAI 180 TACGTA [CT]AC^GT[GA] BsaBI 1642 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1127 CTGCAC GTGCAG(16/14) BsiHKAI 453 GAGCTC G[AT]GC[AT]^C BsiYI 678 CCTTCGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 678 CCTTCGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 1688 GAGACG CGTCTC(1/5) BsmI 807 GAATGC GAATGC(1/-1) BsoBI 839 CTCGAG C^[CT]CG[GA]G BspHI 2135 TCATGA T^CATGA BsrDI 1664 CATTGC GCAATG(2/0) BstBI 209 TTCGAA TT^CGAA BstUI 2090 CGCG CG^CG EaeI 1184 TGGCCG [CT]^GGCC[GA] EcoT14I 310 CCTAGG C^C[AT][AT]GG EcoT22I 299 ATGCAT ATGCA^T HaeII 1859 AGCGCC [GA]GCGC^[CT] HapII 404 CCGG C^CGG HincII 2148 GTTGAC GT[CT]^[GA]AC HindII 2148 GTTGAC GT[CT]^[GA]AC HpaII 404 CCGG C^CGG MamI 1642 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2036 CAATTG C^AATTG MspI 404 CCGG C^CGG MunI 2036 CAATTG C^AATTG MvnI 2090 CGCG CG^CG NciI 404 CCGGG CC^[GC]GG NdeI 2183 CATATG CA^TATG NsiI 299 ATGCAT ATGCA^T NspV 209 TTCGAA TT^CGAA PaeR7I 839 CTCGAG C^TCGAG PshAI 438 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2135 TCATGA T^CATGA SacI 453 GAGCTC GAGCT^C ScaI 946 AGTACT AGT^ACT SfuI 209 TTCGAA TT^CGAA SnaBI 180 TACGTA TAC^GTA StyI 310 CCTAGG C^C[AT][AT]GG XhoI 839 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 1883 CCGC CCGC(-3/-1) 1069 GCGG AseI 1281 ATTAAT AT^TAAT 1915 ATTAAT AsnI 1281 ATTAAT AT^TAAT 1915 ATTAAT Asp700I 1405 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1693 GAAGAAATTC BanII 453 GAGCTC G[GA]GC[CT]^C 851 GAGCCC BclI 54 TGATCA T^GATCA 2040 TGATCA BglII 36 AGATCT A^GATCT 1641 AGATCT BmyI 453 GAGCTC G[AGT]GC[ACT]^C 851 GAGCCC Bsp1286I 453 GAGCTC G[AGT]GC[ACT]^C 851 GAGCCC CfoI 1860 GCGC GCG^C 2089 GCGC Cfr13I 169 GGACC G^G[ACGT]CC 1458 GGGCC DraI 42 TTTAAA TTT^AAA 720 TTTAAA FbaI 54 TGATCA T^GATCA 2040 TGATCA HhaI 1860 GCGC GCG^C 2089 GCGC HinP1I 1860 GCGC G^CGC 2089 GCGC MaeII 181 ACGT A^CGT 1155 ACGT NlaIV 396 GGAGCC GG[ACGT]^[ACGT]CC 530 GGAGCC PshBI 1281 ATTAAT AT^TAAT 1915 ATTAAT Sau96I 169 GGACC G^G[ACGT]CC 1458 GGGCC SmlI 764 CTCAAG C^T[CT][GA]AG 839 CTCGAG XmnI 1405 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1693 GAAGAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI BbvI BfaI BpmI BsaJI BsmAI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII PleI PstI PvuII RsaI Sau3AI ScrFI SfaNI SfcI StuI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII