(Circular) (Six-base) MAPSORT of: pFN21AE2173.seq Check: 4593 from: 1 to: 6898 pFN21AE2173 6898 bp With 182 enzymes: SgfI * March 28, 2012 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4708 278 Size: 53 83 186 936 3172 2468 Fragments arranged by size: 3172 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4241 1937 Size: 2304 4594 Acc65I G'GTAC_C Cuts at: 4219 4219 Size: 6898 AclI AA'CG_TT Cuts at: 1563 5025 5398 1563 Size: 3462 373 3063 Fragments arranged by size: 3462 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2898 3038 3107 3638 4961 6345 6613 1632 Size: 1266 140 69 531 1323 1384 268 1917 Fragments arranged by size: 1917 1384 1323 1266 531 268 140 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6701 AflIII A'CryG_T Cuts at: 1203 5797 6774 1203 Size: 4594 977 1327 Fragments arranged by size: 4594 1327 977 AgeI A'CCGG_T Cuts at: 5729 5729 Size: 6898 AhdI GACnn_n'nnGTC Cuts at: 5627 5627 Size: 6898 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6898 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3974 1184 Size: 2790 4108 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3254 3286 3254 Size: 32 6866 AlwNI CAG_nnn'CTG Cuts at: 2204 2357 2704 2947 3457 3553 4641 6213 2204 Size: 153 347 243 510 96 1088 1572 2889 Fragments arranged by size: 2889 1572 1088 510 347 243 153 96 ApaLI G'TGCA_C Cuts at: 4955 6111 4955 Size: 1156 5742 ApoI r'AATT_y Cuts at: 1493 2524 3166 3702 4068 4207 4433 4585 6694 Size: 1031 642 536 366 139 226 152 2109 Cuts at: 6694 1493 Size: 1697 Fragments arranged by size: 2109 1697 1031 642 536 366 226 152 139 AseI AT'TA_AT Cuts at: 160 1032 3287 3921 5452 160 Size: 872 2255 634 1531 1606 Fragments arranged by size: 2255 1606 1531 872 634 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6898 AvaI C'yCGr_G Cuts at: 1870 1943 2844 4223 1870 Size: 73 901 1379 4545 Fragments arranged by size: 4545 1379 901 73 AvrII C'CTAG_G Cuts at: 2315 2315 Size: 6898 BamHI G'GATC_C Cuts at: 4228 4595 4228 Size: 367 6531 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4219 5674 618 Size: 325 217 627 2432 1455 1842 Fragments arranged by size: 2432 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2462 2860 4217 1391 Size: 1071 398 1357 4072 Fragments arranged by size: 4072 1357 1071 398 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3906 928 Size: 971 2007 3920 Fragments arranged by size: 3920 2007 971 BbvCI CC'TCA_GC Cuts at: 2705 2705 Size: 6898 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3113 3147 5089 5123 1903 Size: 34 1176 34 1942 34 3678 Fragments arranged by size: 3678 1942 1176 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4790 6000 4790 Size: 1210 5688 BclI T'GATC_A Cuts at: 1548 2059 4045 1548 Size: 511 1986 4401 Fragments arranged by size: 4401 1986 511 BfrBI ATG'CAT Cuts at: 2306 2306 Size: 6898 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5509 136 Size: 107 122 71 631 605 3837 1525 Fragments arranged by size: 3837 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2041 3646 6893 2041 Size: 1605 3247 2046 Fragments arranged by size: 3247 2046 1605 BlpI GC'TnA_GC Cuts at: 4315 4315 Size: 6898 Bme1580I G_kGCm'C Cuts at: 1790 4959 6115 1790 Size: 3169 1156 2573 Fragments arranged by size: 3169 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6898 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2487 4629 5587 Size: 416 53 83 399 24 1070 2142 958 Cuts at: 5587 442 Size: 1753 Fragments arranged by size: 2142 1753 1070 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6898 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2711 2912 3629 3839 3881 5558 1239 Size: 1472 201 717 210 42 1677 2579 Fragments arranged by size: 2579 1677 1472 717 210 201 42 Bpu10I CC'TnA_GC Cuts at: 2705 2705 Size: 6898 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2754 5023 5888 6186 2754 Size: 2269 865 298 3466 Fragments arranged by size: 3466 2269 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5561 882 Size: 617 4062 2219 Fragments arranged by size: 4062 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2187 4689 493 Size: 711 983 2502 2702 Fragments arranged by size: 2702 2502 983 711 BsaBI GATnn'nnATC Cuts at: 3651 6892 3651 Size: 3241 3657 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4705 5087 5722 275 Size: 53 83 186 936 3172 382 635 1451 Fragments arranged by size: 3172 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5331 5729 6003 6150 6561 5331 Size: 398 274 147 411 5668 Fragments arranged by size: 5668 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6673 Fragments arranged by size: 6673 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6898 BseYI C'CCAG_C Cuts at: 2901 3569 6101 6597 2901 Size: 668 2532 496 3202 Fragments arranged by size: 3202 2532 668 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3117 1163 Size: 573 1381 4944 Fragments arranged by size: 4944 1381 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4348 5109 6137 1540 Size: 275 186 2347 761 1028 2301 Fragments arranged by size: 2347 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2462 4959 5044 6115 2462 Size: 2497 85 1071 3245 Fragments arranged by size: 3245 2497 1071 85 BsmI GAATG_Cn' Cuts at: 2818 4409 4502 2818 Size: 1591 93 5214 Fragments arranged by size: 5214 1591 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3687 3687 Size: 6898 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2462 2860 4217 4959 5044 6115 1391 Size: 399 672 398 1357 742 85 1071 2174 Fragments arranged by size: 2174 1357 1071 742 672 399 398 85 BspEI T'CCGG_A Cuts at: 6561 6561 Size: 6898 BspHI T'CATG_A Cuts at: 4140 4787 6517 4140 Size: 647 1730 4521 Fragments arranged by size: 4521 1730 647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4253 844 Size: 3409 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4785 1394 Size: 429 2962 3507 Fragments arranged by size: 3507 2962 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3668 5393 5567 65 Size: 1183 2420 1725 174 1396 Fragments arranged by size: 2420 1725 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5542 5729 1700 Size: 110 3732 187 2869 Fragments arranged by size: 3732 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6898 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6898 BssSI C'ACGA_G Cuts at: 4958 5970 4958 Size: 1012 5886 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6898 BstBI TT'CG_AA Cuts at: 2215 5702 2215 Size: 3487 3411 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6898 BstXI CCAn_nnnn'nTGG Cuts at: 3400 3569 4190 3400 Size: 169 621 6108 Fragments arranged by size: 6108 621 169 BstYI r'GATC_y Cuts at: 1972 2041 2264 2756 3482 3550 3646 4228 4595 Size: 69 223 492 726 68 96 582 367 Cuts at: 4595 4980 4997 6449 6535 6547 6893 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 726 582 492 385 367 346 223 96 86 69 68 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6346 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6159 Fragments arranged by size: 6159 683 56 BtsI GCAGTG_nn' Cuts at: 2244 3387 4414 5208 5228 2244 Size: 1143 1027 794 20 3914 Fragments arranged by size: 3914 1143 1027 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6898 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6863 DraI TTT'AAA Cuts at: 2049 2727 4202 2049 Size: 678 1475 4745 Fragments arranged by size: 4745 1475 678 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5905 809 Size: 507 4589 1802 Fragments arranged by size: 4589 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3189 4345 5234 Size: 54 1425 50 275 18 1359 1156 889 Cuts at: 5234 6863 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1359 1156 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4345 1537 Size: 275 2533 4090 Fragments arranged by size: 4090 2533 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3057 3083 3212 4828 1353 Size: 1704 26 129 1616 3423 Fragments arranged by size: 3423 1704 1616 129 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3877 5485 5859 6005 387 Size: 1064 138 2288 1608 374 146 1280 Fragments arranged by size: 2288 1608 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2460 2460 Size: 6898 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2711 2898 2912 3038 3107 3629 3638 Size: 393 1079 187 14 126 69 522 9 Cuts at: 3638 3839 3881 4961 5558 6345 6613 1239 Size: 201 42 1080 597 787 268 1524 Fragments arranged by size: 1524 1080 1079 787 597 522 393 268 201 187 126 69 42 14 9 EcoRI G'AATT_C Cuts at: 4207 4585 4207 Size: 378 6520 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3280 3665 3280 Size: 385 6513 FspI TGC'GCA Cuts at: 5404 5404 Size: 6898 HaeII r_GCGC'y Cuts at: 1678 3868 6045 1678 Size: 2190 2177 2531 Fragments arranged by size: 2531 2190 2177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2648 2680 5539 5571 5613 Size: 32 135 32 1076 32 2859 32 42 Cuts at: 5613 5645 1373 Size: 32 2626 Fragments arranged by size: 2859 2626 1076 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4155 4242 4488 669 Size: 988 281 2217 87 246 3079 Fragments arranged by size: 3079 2217 988 281 246 87 HindIII A'AGCT_T Cuts at: 748 748 Size: 6898 HpaI GTT'AAC Cuts at: 4488 4488 Size: 6898 KpnI G_GTAC'C Cuts at: 4223 4223 Size: 6898 MfeI C'AATT_G Cuts at: 4041 4497 4041 Size: 456 6442 MluI A'CGCG_T Cuts at: 6774 6774 Size: 6898 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6012 6196 1305 Size: 4707 184 2007 Fragments arranged by size: 4707 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5076 Fragments arranged by size: 5076 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4856 5215 5374 518 Size: 648 3690 359 159 2042 Fragments arranged by size: 3690 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2204 2701 2905 4314 4991 6139 6384 1606 Size: 598 497 204 1409 677 1148 245 2120 Fragments arranged by size: 2120 1409 1148 677 598 497 245 204 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6346 NdeI CA'TA_TG Cuts at: 387 4189 387 Size: 3802 3096 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6898 NotI GC'GGCC_GC Cuts at: 4345 4345 Size: 6898 NsiI A_TGCA'T Cuts at: 2308 2308 Size: 6898 NspI r_CATG'y Cuts at: 1132 1229 4256 5801 1132 Size: 97 3027 1545 2229 Fragments arranged by size: 3027 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6898 PciI A'CATG_T Cuts at: 5797 5797 Size: 6898 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5996 PfoI T'CCnGG_A Cuts at: 2928 2928 Size: 6898 PmeI GTTT'AAAC Cuts at: 4202 4202 Size: 6898 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 4967 4999 6480 6512 3875 Size: 32 1060 32 1481 32 4261 Fragments arranged by size: 4261 1481 1060 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2447 1613 Size: 834 6064 PsiI TTA'TAA Cuts at: 4468 4468 Size: 6898 PspXI vC'TCGA_Gb Cuts at: 2844 2844 Size: 6898 PstI C_TGCA'G Cuts at: 830 3033 3048 3237 4250 830 Size: 2203 15 189 1013 3478 Fragments arranged by size: 3478 2203 1013 189 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6898 PvuII CAG'CTG Cuts at: 2204 2701 2905 2204 Size: 497 204 6197 Fragments arranged by size: 6197 497 204 SacI G_AGCT'C Cuts at: 2462 2462 Size: 6898 SalI G'TCGA_C Cuts at: 1936 4240 1936 Size: 2304 4594 SbfI CC_TGCA'GG Cuts at: 4250 4250 Size: 6898 ScaI AGT'ACT Cuts at: 2953 5146 6858 2953 Size: 2193 1712 2993 Fragments arranged by size: 2993 2193 1712 SfcI C'TryA_G Cuts at: 826 1045 3029 3044 3233 4246 5381 6062 6253 Size: 219 1984 15 189 1013 1135 681 191 Cuts at: 6253 826 Size: 1471 Fragments arranged by size: 1984 1471 1135 1013 681 219 191 189 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6898 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6898 SmaI CCC'GGG Cuts at: 1872 4225 1872 Size: 2353 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2769 2844 5002 5903 6165 820 Size: 197 926 826 75 2158 901 262 1553 Fragments arranged by size: 2158 1553 926 901 826 262 197 75 SnaBI TAC'GTA Cuts at: 493 2187 4689 493 Size: 1694 2502 2702 Fragments arranged by size: 2702 2502 1694 SpeI A'CTAG_T Cuts at: 152 152 Size: 6898 SphI G_CATG'C Cuts at: 1132 4256 1132 Size: 3124 3774 SspI AAT'ATT Cuts at: 5 52 4822 5 Size: 47 4770 2081 Fragments arranged by size: 4770 2081 47 StuI AGG'CCT Cuts at: 1517 2285 2320 3375 1517 Size: 768 35 1055 5040 Fragments arranged by size: 5040 1055 768 35 StyI C'CwwG_G Cuts at: 513 1065 2315 4337 513 Size: 552 1250 2022 3074 Fragments arranged by size: 3074 2022 1250 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4925 4942 5095 5280 5725 325 Size: 1255 3345 17 153 185 445 1498 Fragments arranged by size: 3345 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2911 2951 5144 Size: 274 80 33 51 1443 934 40 2193 Cuts at: 5144 6856 96 Size: 1712 138 Fragments arranged by size: 2193 1712 1443 934 274 138 80 51 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6858 6890 6858 Size: 32 6866 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6898 XbaI T'CTAG_A Cuts at: 4234 4234 Size: 6898 XhoI C'TCGA_G Cuts at: 1943 2844 1943 Size: 901 5997 XmaI C'CCGG_G Cuts at: 1870 4223 1870 Size: 2353 4545 XmnI GAAnn'nnTTC Cuts at: 3414 3702 5027 3414 Size: 288 1325 5285 Fragments arranged by size: 5285 1325 288 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4706 276 Size: 53 83 186 936 3172 2468 Fragments arranged by size: 3172 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI