(Circular) (Six-base) MAPSORT of: pFN21AB6486.seq Check: 6610 from: 1 to: 5773 pFN21AB6486 5773 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2596 2596 Size: 5773 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3583 278 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3116 1937 Size: 1179 4594 Acc65I G'GTAC_C Cuts at: 3094 3094 Size: 5773 AclI AA'CG_TT Cuts at: 1563 3900 4273 1563 Size: 2337 373 3063 Fragments arranged by size: 3063 2337 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2705 3836 5220 5488 1632 Size: 1073 1131 1384 268 1917 Fragments arranged by size: 1917 1384 1131 1073 268 AflII C'TTAA_G Cuts at: 820 1017 2717 820 Size: 197 1700 3876 Fragments arranged by size: 3876 1700 197 AflIII A'CryG_T Cuts at: 1203 4672 5649 1203 Size: 3469 977 1327 Fragments arranged by size: 3469 1327 977 AgeI A'CCGG_T Cuts at: 4604 4604 Size: 5773 AhdI GACnn_n'nnGTC Cuts at: 4502 4502 Size: 5773 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5773 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5773 AlwNI CAG_nnn'CTG Cuts at: 3019 3022 3516 5088 3019 Size: 3 494 1572 3704 Fragments arranged by size: 3704 1572 494 3 ApaLI G'TGCA_C Cuts at: 3830 4986 3830 Size: 1156 4617 ApoI r'AATT_y Cuts at: 1493 2017 2884 3082 3308 3460 5569 1493 Size: 524 867 198 226 152 2109 1697 Fragments arranged by size: 2109 1697 867 524 226 198 152 AseI AT'TA_AT Cuts at: 160 1032 4327 160 Size: 872 3295 1606 Fragments arranged by size: 3295 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 AvaI C'yCGr_G Cuts at: 1870 1943 3098 1870 Size: 73 1155 4545 Fragments arranged by size: 4545 1155 73 BamHI G'GATC_C Cuts at: 3103 3470 3103 Size: 367 5406 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2085 3094 4549 618 Size: 325 217 627 298 1009 1455 1842 Fragments arranged by size: 1842 1455 1009 627 325 298 217 BanII G_rGCy'C Cuts at: 1391 2982 3092 1391 Size: 1591 110 4072 Fragments arranged by size: 4072 1591 110 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4802 BbvCI CC'TCA_GC Cuts at: 2442 3036 2442 Size: 594 5179 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3964 3998 1903 Size: 34 2027 34 3678 Fragments arranged by size: 3678 2027 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4875 3665 Size: 1210 4563 BclI T'GATC_A Cuts at: 1548 2835 1548 Size: 1287 4486 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4384 136 Size: 107 122 71 631 605 2712 1525 Fragments arranged by size: 2712 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5768 5768 Size: 5773 BlpI GC'TnA_GC Cuts at: 3190 3190 Size: 5773 Bme1580I G_kGCm'C Cuts at: 1790 2496 3834 4990 1790 Size: 706 1338 1156 2573 Fragments arranged by size: 2573 1338 1156 706 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3504 4462 442 Size: 416 53 83 399 24 2087 958 1753 Fragments arranged by size: 2087 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2059 2566 4433 1239 Size: 820 507 1867 2579 Fragments arranged by size: 2579 1867 820 507 Bpu10I CC'TnA_GC Cuts at: 2433 2442 2976 3036 2433 Size: 9 534 60 5170 Fragments arranged by size: 5170 534 60 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3898 4763 5061 3898 Size: 865 298 4610 Fragments arranged by size: 4610 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2135 4436 882 Size: 617 636 2301 2219 Fragments arranged by size: 2301 2219 636 617 BsaAI yAC'GTr Cuts at: 493 1204 3564 493 Size: 711 2360 2702 Fragments arranged by size: 2702 2360 711 BsaBI GATnn'nnATC Cuts at: 5767 5767 Size: 5773 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3580 3962 4597 275 Size: 53 83 186 936 2047 382 635 1451 Fragments arranged by size: 2047 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4206 4604 4878 5025 5436 4206 Size: 398 274 147 411 4543 Fragments arranged by size: 4543 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5548 Fragments arranged by size: 5548 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5773 BseYI C'CCAG_C Cuts at: 2447 2762 4976 5472 2447 Size: 315 2214 496 2748 Fragments arranged by size: 2748 2214 496 315 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5200 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3223 3984 5012 1540 Size: 275 186 1222 761 1028 2301 Fragments arranged by size: 2301 1222 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2982 3834 3919 4990 2982 Size: 852 85 1071 3765 Fragments arranged by size: 3765 1071 852 85 BsmI GAATG_Cn' Cuts at: 3284 3377 3284 Size: 93 5680 BsmBI CGTCTCn'nnnn_ Cuts at: 2032 2032 Size: 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2496 2982 3092 3834 3919 4990 1391 Size: 399 706 486 110 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 706 486 399 110 85 BspEI T'CCGG_A Cuts at: 5436 5436 Size: 5773 BspHI T'CATG_A Cuts at: 3004 3662 5392 3004 Size: 658 1730 3385 Fragments arranged by size: 3385 1730 658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2215 2578 2596 3128 844 Size: 1371 363 18 532 3489 Fragments arranged by size: 3489 1371 532 363 18 BsrBI CCG'CTC Cuts at: 1394 1823 3660 1394 Size: 429 1837 3507 Fragments arranged by size: 3507 1837 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4268 4442 65 Size: 1183 3020 174 1396 Fragments arranged by size: 3020 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2082 4417 4604 1700 Size: 110 272 2335 187 2869 Fragments arranged by size: 2869 2335 272 187 110 BsrGI T'GTAC_A Cuts at: 96 2821 96 Size: 2725 3048 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5773 BssSI C'ACGA_G Cuts at: 3833 4845 3833 Size: 1012 4761 BstAPI GCAn_nnn'nTGC Cuts at: 794 3022 794 Size: 2228 3545 BstBI TT'CG_AA Cuts at: 4577 4577 Size: 5773 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5773 BstYI r'GATC_y Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768 Size: 1131 367 385 17 1452 86 12 346 Cuts at: 5768 1972 Size: 1977 Fragments arranged by size: 1977 1452 1131 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2663 513 Size: 552 1598 3623 Fragments arranged by size: 3623 1598 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5034 Fragments arranged by size: 5034 683 56 BtsI GCAGTG_nn' Cuts at: 3289 4083 4103 3289 Size: 794 20 4959 Fragments arranged by size: 4959 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2230 2265 529 Size: 35 1666 35 4037 Fragments arranged by size: 4037 1666 35 35 DraI TTT'AAA Cuts at: 3077 3077 Size: 5773 DraIII CAC_nnn'GTG Cuts at: 2314 2734 2314 Size: 420 5353 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4780 809 Size: 507 3464 1802 Fragments arranged by size: 3464 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2296 2479 2694 Size: 54 1425 50 275 18 466 183 215 Cuts at: 2694 3220 4109 5738 8 Size: 526 889 1629 43 Fragments arranged by size: 1629 1425 889 526 466 275 215 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3220 1537 Size: 275 1408 4090 Fragments arranged by size: 4090 1408 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2346 2381 3703 1353 Size: 993 35 1322 3423 Fragments arranged by size: 3423 1322 993 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4360 4734 4880 387 Size: 1064 138 2771 374 146 1280 Fragments arranged by size: 2771 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2980 2980 Size: 5773 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2566 2705 3836 4433 5220 5488 Size: 393 427 507 139 1131 597 787 268 Cuts at: 5488 1239 Size: 1524 Fragments arranged by size: 1524 1131 787 597 507 427 393 268 139 EcoNI CCTnn'n_nnAGG Cuts at: 2501 2501 Size: 5773 EcoO109I rG'GnC_Cy Cuts at: 2575 2575 Size: 5773 EcoRI G'AATT_C Cuts at: 2884 3082 3460 2884 Size: 198 378 5197 Fragments arranged by size: 5197 378 198 FspI TGC'GCA Cuts at: 4279 4279 Size: 5773 HaeII r_GCGC'y Cuts at: 1678 4920 1678 Size: 3242 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2943 2975 4414 4446 4488 Size: 32 135 32 1371 32 1439 32 42 Cuts at: 4488 4520 1373 Size: 32 2626 Fragments arranged by size: 2626 1439 1371 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3117 3363 669 Size: 988 281 1179 246 3079 Fragments arranged by size: 3079 1179 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5773 HpaI GTT'AAC Cuts at: 3363 3363 Size: 5773 KpnI G_GTAC'C Cuts at: 3098 3098 Size: 5773 MfeI C'AATT_G Cuts at: 3372 3372 Size: 5773 MluI A'CGCG_T Cuts at: 5649 5649 Size: 5773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2055 4887 5071 1305 Size: 750 2832 184 2007 Fragments arranged by size: 2832 2007 750 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2298 2481 2696 10 Size: 54 1425 343 466 183 215 3087 Fragments arranged by size: 3087 1425 466 343 215 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2829 3731 4090 4249 518 Size: 648 1663 902 359 159 2042 Fragments arranged by size: 2042 1663 902 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3019 3040 3189 3866 5014 5259 1606 Size: 1413 21 149 677 1148 245 2120 Fragments arranged by size: 2120 1413 1148 677 245 149 21 NaeI GCC'GGC Cuts at: 2084 2084 Size: 5773 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5221 NdeI CA'TA_TG Cuts at: 387 2724 387 Size: 2337 3436 NgoMIV G'CCGG_C Cuts at: 2082 2082 Size: 5773 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5773 NotI GC'GGCC_GC Cuts at: 3220 3220 Size: 5773 NspI r_CATG'y Cuts at: 1132 1229 3131 4676 1132 Size: 97 1902 1545 2229 Fragments arranged by size: 2229 1902 1545 97 PasI CC'CwG_GG Cuts at: 1348 2536 1348 Size: 1188 4585 PciI A'CATG_T Cuts at: 4672 4672 Size: 5773 PflMI CCAn_nnn'nTGG Cuts at: 1108 2653 1108 Size: 1545 4228 PmeI GTTT'AAAC Cuts at: 3077 3077 Size: 5773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3842 3874 5355 5387 3842 Size: 32 1481 32 4228 Fragments arranged by size: 4228 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2575 2575 Size: 5773 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5773 PsiI TTA'TAA Cuts at: 3343 3343 Size: 5773 PstI C_TGCA'G Cuts at: 830 2292 3125 830 Size: 1462 833 3478 Fragments arranged by size: 3478 1462 833 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5773 PvuII CAG'CTG Cuts at: 3019 3040 3019 Size: 21 5752 SacI G_AGCT'C Cuts at: 2982 2982 Size: 5773 SalI G'TCGA_C Cuts at: 1936 3115 1936 Size: 1179 4594 SbfI CC_TGCA'GG Cuts at: 3125 3125 Size: 5773 ScaI AGT'ACT Cuts at: 4021 5733 4021 Size: 1712 4061 SfcI C'TryA_G Cuts at: 826 1045 2288 2415 3121 4256 4937 5128 826 Size: 219 1243 127 706 1135 681 191 1471 Fragments arranged by size: 1471 1243 1135 706 681 219 191 127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5773 SmaI CCC'GGG Cuts at: 1872 3100 1872 Size: 1228 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2717 3877 4778 5040 820 Size: 197 926 774 1160 901 262 1553 Fragments arranged by size: 1553 1160 926 901 774 262 197 SnaBI TAC'GTA Cuts at: 493 3564 493 Size: 3071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5773 SphI G_CATG'C Cuts at: 1132 3131 1132 Size: 1999 3774 SspI AAT'ATT Cuts at: 5 52 3697 5 Size: 47 3645 2081 Fragments arranged by size: 3645 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5773 StyI C'CwwG_G Cuts at: 513 1065 3212 513 Size: 552 2147 3074 Fragments arranged by size: 3074 2147 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3800 3817 3970 4155 4600 325 Size: 1255 2220 17 153 185 445 1498 Fragments arranged by size: 2220 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2179 2821 4019 Size: 274 80 33 51 1443 202 642 1198 Cuts at: 4019 5731 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1198 642 274 202 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5733 5765 5733 Size: 32 5741 Tth111I GACn'n_nGTC Cuts at: 1316 2255 1316 Size: 939 4834 XbaI T'CTAG_A Cuts at: 3109 3109 Size: 5773 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5773 XmaI C'CCGG_G Cuts at: 1870 3098 1870 Size: 1228 4545 XmnI GAAnn'nnTTC Cuts at: 2888 3902 2888 Size: 1014 4759 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3581 276 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI