(Circular) (Six-base) MAPSORT of: pFN21AB6831.seq Check: 3795 from: 1 to: 5203 pFN21AB6831 5203 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3013 278 Size: 53 83 186 936 1477 2468 Fragments arranged by size: 2468 1477 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2546 1937 Size: 609 4594 Acc65I G'GTAC_C Cuts at: 2524 2524 Size: 5203 AclI AA'CG_TT Cuts at: 1563 3330 3703 1563 Size: 1767 373 3063 Fragments arranged by size: 3063 1767 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3266 4650 4918 1632 Size: 1634 1384 268 1917 Fragments arranged by size: 1917 1634 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5006 AflIII A'CryG_T Cuts at: 1203 4102 5079 1203 Size: 2899 977 1327 Fragments arranged by size: 2899 1327 977 AgeI A'CCGG_T Cuts at: 4034 4034 Size: 5203 AhdI GACnn_n'nnGTC Cuts at: 3932 3932 Size: 5203 AleI CACnn'nnGTG Cuts at: 1166 2039 1166 Size: 873 4330 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5203 AlwNI CAG_nnn'CTG Cuts at: 2946 4518 2946 Size: 1572 3631 ApaLI G'TGCA_C Cuts at: 3260 4416 3260 Size: 1156 4047 ApoI r'AATT_y Cuts at: 1493 2512 2738 2890 4999 1493 Size: 1019 226 152 2109 1697 Fragments arranged by size: 2109 1697 1019 226 152 AseI AT'TA_AT Cuts at: 160 1032 3757 160 Size: 872 2725 1606 Fragments arranged by size: 2725 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5203 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2108 2528 1870 Size: 73 134 31 420 4545 Fragments arranged by size: 4545 420 134 73 31 BamHI G'GATC_C Cuts at: 2533 2900 2533 Size: 367 4836 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2386 2524 3979 618 Size: 325 217 627 599 138 1455 1842 Fragments arranged by size: 1842 1455 627 599 325 217 138 BanII G_rGCy'C Cuts at: 1391 2472 2522 1391 Size: 1081 50 4072 Fragments arranged by size: 4072 1081 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2158 928 Size: 971 259 3973 Fragments arranged by size: 3973 971 259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3394 3428 1903 Size: 34 1457 34 3678 Fragments arranged by size: 3678 1457 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3095 4305 3095 Size: 1210 3993 BclI T'GATC_A Cuts at: 1548 2295 1548 Size: 747 4456 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3814 136 Size: 107 122 71 631 605 337 1805 1525 Fragments arranged by size: 1805 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5198 5198 Size: 5203 BlpI GC'TnA_GC Cuts at: 2620 2620 Size: 5203 Bme1580I G_kGCm'C Cuts at: 1790 2391 3264 4420 1790 Size: 601 873 1156 2573 Fragments arranged by size: 2573 1156 873 601 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5203 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2934 3892 442 Size: 416 53 83 399 24 1517 958 1753 Fragments arranged by size: 1753 1517 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5203 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2103 2103 Size: 5203 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3863 1239 Size: 2624 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3328 4193 4491 3328 Size: 865 298 4040 Fragments arranged by size: 4040 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2119 2420 3866 882 Size: 617 620 301 1446 2219 Fragments arranged by size: 2219 1446 620 617 301 BsaAI yAC'GTr Cuts at: 493 1204 2994 493 Size: 711 1790 2702 Fragments arranged by size: 2702 1790 711 BsaBI GATnn'nnATC Cuts at: 5197 5197 Size: 5203 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2083 3010 3392 4027 Size: 53 83 186 936 550 927 382 635 Cuts at: 4027 275 Size: 1451 Fragments arranged by size: 1451 936 927 635 550 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2017 3636 4034 4308 4455 4866 2017 Size: 1619 398 274 147 411 2354 Fragments arranged by size: 2354 1619 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2073 2103 1178 Size: 30 165 30 670 30 4278 Fragments arranged by size: 4278 670 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2213 2246 2249 2258 1186 Size: 895 132 33 3 9 4131 Fragments arranged by size: 4131 895 132 33 9 3 BseYI C'CCAG_C Cuts at: 2128 2307 4406 4902 2128 Size: 179 2099 496 2429 Fragments arranged by size: 2429 2099 496 179 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4630 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2653 3414 4442 1540 Size: 275 186 652 761 1028 2301 Fragments arranged by size: 2301 1028 761 652 275 186 BsiHKAI G_wGCw'C Cuts at: 2472 3264 3349 4420 2472 Size: 792 85 1071 3255 Fragments arranged by size: 3255 1071 792 85 BsmI GAATG_Cn' Cuts at: 2714 2807 2714 Size: 93 5110 BsmBI CGTCTCn'nnnn_ Cuts at: 2074 2074 Size: 5203 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2391 2472 2522 3264 3349 4420 1391 Size: 399 601 81 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 601 399 85 81 50 BspEI T'CCGG_A Cuts at: 2017 4866 2017 Size: 2849 2354 BspHI T'CATG_A Cuts at: 2460 3092 4822 2460 Size: 632 1730 2841 Fragments arranged by size: 2841 1730 632 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2558 844 Size: 1714 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3090 1394 Size: 429 1267 3507 Fragments arranged by size: 3507 1267 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3698 3872 65 Size: 1183 2450 174 1396 Fragments arranged by size: 2450 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3847 4034 1700 Size: 110 2037 187 2869 Fragments arranged by size: 2869 2037 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5203 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5203 BssSI C'ACGA_G Cuts at: 3263 4275 3263 Size: 1012 4191 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5203 BstBI TT'CG_AA Cuts at: 4007 4007 Size: 5203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5203 BstXI CCAn_nnnn'nTGG Cuts at: 2164 2164 Size: 5203 BstYI r'GATC_y Cuts at: 1972 2533 2900 3285 3302 4754 4840 4852 5198 Size: 561 367 385 17 1452 86 12 346 Cuts at: 5198 1972 Size: 1977 Fragments arranged by size: 1977 1452 561 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3712 Fragments arranged by size: 3712 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4464 Fragments arranged by size: 4464 683 56 BtsI GCAGTG_nn' Cuts at: 2719 3513 3533 2719 Size: 794 20 4389 Fragments arranged by size: 4389 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5203 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5168 DraI TTT'AAA Cuts at: 2507 2507 Size: 5203 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4210 809 Size: 507 2894 1802 Fragments arranged by size: 2894 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2439 2650 3539 Size: 54 1425 50 275 18 609 211 889 Cuts at: 3539 5168 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 609 275 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2650 1537 Size: 275 838 4090 Fragments arranged by size: 4090 838 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 2190 2223 2235 2248 3133 1353 Size: 819 18 33 12 13 885 3423 Fragments arranged by size: 3423 885 819 33 18 13 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2423 3790 4164 4310 387 Size: 1064 138 834 1367 374 146 1280 Fragments arranged by size: 1367 1280 1064 834 374 146 138 EcoICRI GAG'CTC Cuts at: 2470 2470 Size: 5203 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3266 3863 4650 4918 1239 Size: 393 1634 597 787 268 1524 Fragments arranged by size: 1634 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2357 2357 Size: 5203 EcoO109I rG'GnC_Cy Cuts at: 2125 2185 2125 Size: 60 5143 EcoRI G'AATT_C Cuts at: 2512 2890 2512 Size: 378 4825 FspI TGC'GCA Cuts at: 3709 3709 Size: 5203 HaeII r_GCGC'y Cuts at: 1678 2314 4350 1678 Size: 636 2036 2531 Fragments arranged by size: 2531 2036 636 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2073 2105 3844 3876 3918 Size: 32 135 32 501 32 1739 32 42 Cuts at: 3918 3950 1373 Size: 32 2626 Fragments arranged by size: 2626 1739 501 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2547 2793 669 Size: 988 281 609 246 3079 Fragments arranged by size: 3079 988 609 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5203 HpaI GTT'AAC Cuts at: 2793 2793 Size: 5203 KpnI G_GTAC'C Cuts at: 2528 2528 Size: 5203 MfeI C'AATT_G Cuts at: 2802 2802 Size: 5203 MluI A'CGCG_T Cuts at: 5079 5079 Size: 5203 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4317 4501 1305 Size: 3012 184 2007 Fragments arranged by size: 3012 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2441 10 Size: 54 1425 343 609 2772 Fragments arranged by size: 2772 1425 609 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 3161 3520 3679 518 Size: 648 873 1122 359 159 2042 Fragments arranged by size: 2042 1122 873 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2374 2619 3296 4444 4689 1606 Size: 768 245 677 1148 245 2120 Fragments arranged by size: 2120 1148 768 677 245 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3712 Fragments arranged by size: 3712 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5203 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5203 NotI GC'GGCC_GC Cuts at: 2650 2650 Size: 5203 NspI r_CATG'y Cuts at: 1132 1229 2561 4106 1132 Size: 97 1332 1545 2229 Fragments arranged by size: 2229 1545 1332 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5203 PciI A'CATG_T Cuts at: 4102 4102 Size: 5203 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5203 PfoI T'CCnGG_A Cuts at: 2417 2417 Size: 5203 PmeI GTTT'AAAC Cuts at: 2507 2507 Size: 5203 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3272 3304 4785 4817 3272 Size: 32 1481 32 3658 Fragments arranged by size: 3658 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2185 2125 Size: 60 5143 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5203 PsiI TTA'TAA Cuts at: 2773 2773 Size: 5203 PstI C_TGCA'G Cuts at: 830 2555 830 Size: 1725 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5203 PvuII CAG'CTG Cuts at: 2374 2374 Size: 5203 SacI G_AGCT'C Cuts at: 2472 2472 Size: 5203 SalI G'TCGA_C Cuts at: 1936 2545 1936 Size: 609 4594 SanDI GG'GwC_CC Cuts at: 2125 2185 2125 Size: 60 5143 SbfI CC_TGCA'GG Cuts at: 2555 2555 Size: 5203 ScaI AGT'ACT Cuts at: 3451 5163 3451 Size: 1712 3491 SfcI C'TryA_G Cuts at: 826 1045 2551 3686 4367 4558 826 Size: 219 1506 1135 681 191 1471 Fragments arranged by size: 1506 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5203 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5203 SmaI CCC'GGG Cuts at: 1872 2110 2530 1872 Size: 238 420 4545 Fragments arranged by size: 4545 420 238 SmlI C'TyrA_G Cuts at: 820 1017 1943 3307 4208 4470 820 Size: 197 926 1364 901 262 1553 Fragments arranged by size: 1553 1364 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2994 493 Size: 2501 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5203 SphI G_CATG'C Cuts at: 1132 2561 1132 Size: 1429 3774 SspI AAT'ATT Cuts at: 5 52 3127 5 Size: 47 3075 2081 Fragments arranged by size: 3075 2081 47 StuI AGG'CCT Cuts at: 1517 2363 1517 Size: 846 4357 StyI C'CwwG_G Cuts at: 513 1065 2004 2118 2642 513 Size: 552 939 114 524 3074 Fragments arranged by size: 3074 939 552 524 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2480 3230 3247 3400 3585 4030 325 Size: 1255 900 750 17 153 185 445 1498 Fragments arranged by size: 1498 1255 900 750 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3449 5161 96 Size: 274 80 33 51 1443 1472 1712 138 Fragments arranged by size: 1712 1472 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5163 5195 5163 Size: 32 5171 Tth111I GACn'n_nGTC Cuts at: 1316 2051 1316 Size: 735 4468 XbaI T'CTAG_A Cuts at: 2539 2539 Size: 5203 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5203 XmaI C'CCGG_G Cuts at: 1870 2108 2528 1870 Size: 238 420 4545 Fragments arranged by size: 4545 420 238 XmnI GAAnn'nnTTC Cuts at: 3332 3332 Size: 5203 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3011 276 Size: 53 83 186 936 1477 2468 Fragments arranged by size: 2468 1477 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI