Restriction map for
pFN21AB6820
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# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssKI Bst1107I BstAPI BstEII
BstNI BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT22I FbaI FseI
FspI HaeIII HapII Hin1I HincII
HindII HpaI HpaII HphI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MseI MspA1I
MspI MunI MvaI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI Sau96I SbfI ScrFI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 120 CGCG CG^CG
AfaI 87 GTAC GT^AC
AvaI 90 CTCGGG C^[CT]CG[GA]G
BsaJI 410 CCTTGG C^C[ACGT][ACGT]GG
BseRI 260 GAGGAG GAGGAG(10/8)
BsmFI 209 GGGAC GGGAC(10/14)
BsmI 537 GCATTC GAATGC(1/-1)
BsoBI 90 CTCGGG C^[CT]CG[GA]G
BsrBI 247 GAGCGG CCGCTC(-3/-3)
BssSI 73 CTCGTG CACGAG(-5/-1)
BstBI 233 TTCGAA TT^CGAA
BstF5I 414 GGATG GGATG(2/0)
BstUI 120 CGCG CG^CG
CfoI 7 GCGC GCG^C
EcoRI 365 GAATTC G^AATTC
EcoRV 511 GATATC GAT^ATC
EcoT14I 410 CCTTGG C^C[AT][AT]GG
FauI 17 CCCGC CCCGC(4/6)
FokI 414 GGATG GGATG(9/13)
HaeII 6 GGCGCT [GA]GCGC^[CT]
HhaI 7 GCGC GCG^C
HinP1I 7 GCGC G^CGC
HindIII 314 AAGCTT A^AGCTT
MaeII 191 ACGT A^CGT
MslI 518 CACTTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 120 CGCG CG^CG
NlaIV 32 GGTTCC GG[ACGT]^[ACGT]CC
NspI 239 GCATGT [GA]CATG^[CT]
NspV 233 TTCGAA TT^CGAA
PleI 263 GAGTC GAGTC(4/5)
RsaI 87 GTAC GT^AC
SapI 42 GCTCTTC GCTCTTC(1/4)
ScaI 86 AGTACT AGT^ACT
SfaNI 289 GATGC GCATC(5/9)
SfuI 233 TTCGAA TT^CGAA
StyI 410 CCTTGG C^C[AT][AT]GG
TaqI 234 TCGA T^CGA
TfiI 441 GAATC G^A[AT]TC
Tsp45I 193 GTCAC ^GT[GC]AC
TthHB8I 234 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 18 CCGC CCGC(-3/-1)
249 GCGG
AcsI 365 GAATTC [GA]^AATT[CT]
467 AAATTT
ApoI 365 GAATTC [GA]^AATT[CT]
467 AAATTT
BglII 203 AGATCT A^GATCT
396 AGATCT
BsiYI 18 CCGCCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
151 CCCACAGTGGG
BslI 18 CCGCCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
151 CCCACAGTGGG
BsrI 29 ACTGG ACTGG(1/-1)
370 CCAGT
BstYI 203 AGATCT [GA]^GATC[CT]
396 AGATCT
Cac8I 3 GCTGGC GC[ACGT]^[ACGT]GC
20 GCCTGC
EarI 43 CTCTTC CTCTTC(1/4)
55 GAAGAG
HgaI 69 GACGC GACGC(5/10)
121 GCGTC
HinfI 263 GAGTC G^A[ACGT]TC
441 GAATC
Ksp632I 43 CTCTTC CTCTTC(1/4)
55 GAAGAG
MflI 203 AGATCT [GA]^GATC[CT]
396 AGATCT
MwoI 72 GCTCGTGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
528 GCTTATTCAGC
PstI 82 CTGCAG CTGCA^G
293 CTGCAG
SfcI 82 CTGCAG C^T[GA][CT]AG
293 CTGCAG
XbaI 342 TCTAGA T^CTAGA
547 TCTAGA
XhoII 203 AGATCT [GA]^GATC[CT]
396 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BbvI BfaI DpnII Fnu4HI
ItaI MaeI MaeIII MboI MboII
MnlI NdeII NlaIII Sau3AI TseI
Tsp509I TspRI