(Circular) (Six-base) MAPSORT of: pFN21AE3190.seq Check: 6169 from: 1 to: 6031 pFN21AE3190 6031 bp With 182 enzymes: SgfI * July 14, 2018 22:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2064 2064 Size: 6031 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3841 278 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3374 1937 Size: 1437 4594 Acc65I G'GTAC_C Cuts at: 3352 3352 Size: 6031 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 3312 4094 5478 5746 1632 Size: 653 1027 782 1384 268 1917 Fragments arranged by size: 1917 1384 1027 782 653 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5834 AflIII A'CryG_T Cuts at: 1203 3228 4930 5907 1203 Size: 2025 1702 977 1327 Fragments arranged by size: 2025 1702 1327 977 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 4760 4760 Size: 6031 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6031 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2049 2072 2234 1184 Size: 865 23 162 4981 Fragments arranged by size: 4981 865 162 23 AlwNI CAG_nnn'CTG Cuts at: 3290 3774 5346 3290 Size: 484 1572 3975 Fragments arranged by size: 3975 1572 484 ApaLI G'TGCA_C Cuts at: 4088 5244 4088 Size: 1156 4875 ApoI r'AATT_y Cuts at: 1493 2146 2201 2338 2553 2889 3242 3340 3566 Size: 653 55 137 215 336 353 98 226 Cuts at: 3566 3718 5827 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 653 353 336 226 215 152 137 98 55 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 3356 1870 Size: 73 1413 4545 Fragments arranged by size: 4545 1413 73 AvrII C'CTAG_G Cuts at: 2182 2182 Size: 6031 BamHI G'GATC_C Cuts at: 3361 3728 3361 Size: 367 5664 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3352 4807 618 Size: 325 217 627 1565 1455 1842 Fragments arranged by size: 1842 1565 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3350 1391 Size: 1959 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2112 2494 2658 2858 928 Size: 971 213 382 164 200 4101 Fragments arranged by size: 4101 971 382 213 200 164 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4222 4256 1903 Size: 34 2285 34 3678 Fragments arranged by size: 3678 2285 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3923 5133 3923 Size: 1210 4821 BclI T'GATC_A Cuts at: 1548 1548 Size: 6031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2731 4642 136 Size: 107 122 71 631 605 1059 1911 1525 Fragments arranged by size: 1911 1525 1059 631 605 122 107 71 BglII A'GATC_T Cuts at: 3180 6026 3180 Size: 2846 3185 BlpI GC'TnA_GC Cuts at: 3448 3448 Size: 6031 Bme1580I G_kGCm'C Cuts at: 1790 4092 5248 1790 Size: 2302 1156 2573 Fragments arranged by size: 2573 2302 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6031 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3166 3762 4720 Size: 416 53 83 399 24 1749 596 958 Cuts at: 4720 442 Size: 1753 Fragments arranged by size: 1753 1749 958 596 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4691 1239 Size: 3452 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2301 2528 2629 4156 5021 5319 2301 Size: 227 101 1527 865 298 3013 Fragments arranged by size: 3013 1527 865 298 227 101 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2750 4694 882 Size: 617 1251 1944 2219 Fragments arranged by size: 2219 1944 1251 617 BsaAI yAC'GTr Cuts at: 493 1204 2789 3822 493 Size: 711 1585 1033 2702 Fragments arranged by size: 2702 1585 1033 711 BsaBI GATnn'nnATC Cuts at: 2270 2802 6025 2270 Size: 532 3223 2276 Fragments arranged by size: 3223 2276 532 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3838 4220 4855 275 Size: 53 83 186 936 2305 382 635 1451 Fragments arranged by size: 2305 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4464 4862 5136 5283 5694 4464 Size: 398 274 147 411 4801 Fragments arranged by size: 4801 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5806 Fragments arranged by size: 5806 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6031 BseYI C'CCAG_C Cuts at: 5234 5730 5234 Size: 496 5535 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5458 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3481 4242 5270 1540 Size: 275 186 1480 761 1028 2301 Fragments arranged by size: 2301 1480 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4092 4177 5248 4092 Size: 85 1071 4875 Fragments arranged by size: 4875 1071 85 BsmI GAATG_Cn' Cuts at: 3542 3635 3542 Size: 93 5938 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3350 4092 4177 5248 1391 Size: 399 1560 742 85 1071 2174 Fragments arranged by size: 2174 1560 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5694 5694 Size: 6031 BspHI T'CATG_A Cuts at: 3920 5650 3920 Size: 1730 4301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2064 3386 844 Size: 1220 1322 3489 Fragments arranged by size: 3489 1322 1220 BsrBI CCG'CTC Cuts at: 1394 1823 2062 3918 1394 Size: 429 239 1856 3507 Fragments arranged by size: 3507 1856 429 239 BsrDI GCAATG_nn' Cuts at: 65 1248 2389 4526 4700 65 Size: 1183 1141 2137 174 1396 Fragments arranged by size: 2137 1396 1183 1141 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 3120 4675 4862 1700 Size: 110 199 1111 1555 187 2869 Fragments arranged by size: 2869 1555 1111 199 187 110 BsrGI T'GTAC_A Cuts at: 96 2386 96 Size: 2290 3741 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6031 BssSI C'ACGA_G Cuts at: 4091 5103 4091 Size: 1012 5019 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6031 BstBI TT'CG_AA Cuts at: 4835 4835 Size: 6031 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6031 BstYI r'GATC_y Cuts at: 1972 3180 3361 3728 4113 4130 5582 5668 5680 Size: 1208 181 367 385 17 1452 86 12 Cuts at: 5680 6026 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1208 385 367 346 181 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4540 Fragments arranged by size: 4540 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5292 Fragments arranged by size: 5292 683 56 BtsI GCAGTG_nn' Cuts at: 2917 3547 4341 4361 2917 Size: 630 794 20 4587 Fragments arranged by size: 4587 794 630 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5996 DraI TTT'AAA Cuts at: 3335 3335 Size: 6031 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5038 809 Size: 507 3722 1802 Fragments arranged by size: 3722 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3213 3478 Size: 54 1425 50 275 18 177 1206 265 Cuts at: 3478 4367 5996 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1206 889 275 265 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3478 1537 Size: 275 1666 4090 Fragments arranged by size: 4090 1666 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2304 2684 2826 3961 1353 Size: 951 380 142 1135 3423 Fragments arranged by size: 3423 1135 951 380 142 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4618 4992 5138 387 Size: 1064 138 3029 374 146 1280 Fragments arranged by size: 3029 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 3312 4094 4691 5478 5746 1239 Size: 393 653 1027 782 597 787 268 1524 Fragments arranged by size: 1524 1027 787 782 653 597 393 268 EcoRI G'AATT_C Cuts at: 2889 3340 3718 2889 Size: 451 378 5202 Fragments arranged by size: 5202 451 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3338 3338 Size: 6031 FseI GG_CCGG'CC Cuts at: 3124 3124 Size: 6031 FspI TGC'GCA Cuts at: 2037 4537 2037 Size: 2500 3531 HaeII r_GCGC'y Cuts at: 1678 5178 1678 Size: 3500 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2281 2313 4672 4704 4746 Size: 32 135 32 709 32 2359 32 42 Cuts at: 4746 4778 1373 Size: 32 2626 Fragments arranged by size: 2626 2359 709 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3375 3621 669 Size: 988 281 1437 246 3079 Fragments arranged by size: 3079 1437 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6031 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KpnI G_GTAC'C Cuts at: 3356 3356 Size: 6031 MfeI C'AATT_G Cuts at: 2481 3630 2481 Size: 1149 4882 MluI A'CGCG_T Cuts at: 5907 5907 Size: 6031 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5145 5329 1305 Size: 3840 184 2007 Fragments arranged by size: 3840 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4209 Fragments arranged by size: 4209 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2668 3066 3989 4348 4507 518 Size: 648 1502 398 923 359 159 2042 Fragments arranged by size: 2042 1502 923 648 398 359 159 MspA1I CmG'CkG Cuts at: 1606 2235 3141 3447 4124 5272 5517 1606 Size: 629 906 306 677 1148 245 2120 Fragments arranged by size: 2120 1148 906 677 629 306 245 NaeI GCC'GGC Cuts at: 2011 3122 2011 Size: 1111 4920 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4540 Fragments arranged by size: 4540 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6031 NgoMIV G'CCGG_C Cuts at: 2009 3120 2009 Size: 1111 4920 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6031 NotI GC'GGCC_GC Cuts at: 3478 3478 Size: 6031 NspI r_CATG'y Cuts at: 1132 1229 3232 3389 4934 1132 Size: 97 2003 157 1545 2229 Fragments arranged by size: 2229 2003 1545 157 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6031 PciI A'CATG_T Cuts at: 3228 4930 3228 Size: 1702 4329 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6031 PfoI T'CCnGG_A Cuts at: 2281 2281 Size: 6031 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4100 4132 5613 5645 4100 Size: 32 1481 32 4486 Fragments arranged by size: 4486 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2157 2235 1613 Size: 544 78 5409 Fragments arranged by size: 5409 544 78 PsiI TTA'TAA Cuts at: 3601 3601 Size: 6031 PstI C_TGCA'G Cuts at: 830 2074 2593 3383 830 Size: 1244 519 790 3478 Fragments arranged by size: 3478 1244 790 519 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6031 PvuII CAG'CTG Cuts at: 2235 3141 2235 Size: 906 5125 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SbfI CC_TGCA'GG Cuts at: 3383 3383 Size: 6031 ScaI AGT'ACT Cuts at: 4279 5991 4279 Size: 1712 4319 SfcI C'TryA_G Cuts at: 826 1045 2070 2589 3129 3379 4514 5195 5386 Size: 219 1025 519 540 250 1135 681 191 Cuts at: 5386 826 Size: 1471 Fragments arranged by size: 1471 1135 1025 681 540 519 250 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6031 SmaI CCC'GGG Cuts at: 1872 3358 1872 Size: 1486 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2316 2507 2644 4135 5036 5298 Size: 197 926 373 191 137 1491 901 262 Cuts at: 5298 820 Size: 1553 Fragments arranged by size: 1553 1491 926 901 373 262 197 191 137 SnaBI TAC'GTA Cuts at: 493 3822 493 Size: 3329 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6031 SphI G_CATG'C Cuts at: 1132 3389 1132 Size: 2257 3774 SspI AAT'ATT Cuts at: 5 52 3015 3955 5 Size: 47 2963 940 2081 Fragments arranged by size: 2963 2081 940 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6031 StyI C'CwwG_G Cuts at: 513 1065 2004 2182 3470 513 Size: 552 939 178 1288 3074 Fragments arranged by size: 3074 1288 939 552 178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4058 4075 4228 4413 4858 325 Size: 1255 2478 17 153 185 445 1498 Fragments arranged by size: 2478 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2386 2957 4277 Size: 274 80 33 51 1443 409 571 1320 Cuts at: 4277 5989 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1320 571 409 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5991 6023 5991 Size: 32 5999 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6031 XbaI T'CTAG_A Cuts at: 3367 3367 Size: 6031 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 3356 1870 Size: 1486 4545 XmnI GAAnn'nnTTC Cuts at: 2202 4160 2202 Size: 1958 4073 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3839 276 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI