Restriction map for
pFN21AB7530
(Circular) (Six-base) MAPSORT of: pFN21AB7530.seq Check: 9367 from: 1 to: 5470
pFN21AB7530 5470 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2416 2416
Size: 5470
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3280 278
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2813 1937
Size: 876 4594
Acc65I G'GTAC_C
Cuts at: 2791 2791
Size: 5470
AclI AA'CG_TT
Cuts at: 1563 3597 3970 1563
Size: 2034 373 3063
Fragments arranged by size:
3063 2034 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 3533 4917 5185 1632
Size: 680 1221 1384 268 1917
Fragments arranged by size:
1917 1384 1221 680 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5273
AflIII A'CryG_T
Cuts at: 1203 4369 5346 1203
Size: 3166 977 1327
Fragments arranged by size:
3166 1327 977
AgeI A'CCGG_T
Cuts at: 4301 4301
Size: 5470
AhdI GACnn_n'nnGTC
Cuts at: 4199 4199
Size: 5470
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5470
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5470
AlwNI CAG_nnn'CTG
Cuts at: 2356 2683 3213 4785 2356
Size: 327 530 1572 3041
Fragments arranged by size:
3041 1572 530 327
ApaLI G'TGCA_C
Cuts at: 3527 4683 3527
Size: 1156 4314
ApoI r'AATT_y
Cuts at: 1493 2084 2779 3005 3157 5266 1493
Size: 591 695 226 152 2109 1697
Fragments arranged by size:
2109 1697 695 591 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4024 160
Size: 872 2992 1606
Fragments arranged by size:
2992 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2795 1870
Size: 73 132 720 4545
Fragments arranged by size:
4545 720 132 73
BamHI G'GATC_C
Cuts at: 2800 3167 2800
Size: 367 5103
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2286 2791 4246 618
Size: 325 217 627 499 505 1455 1842
Fragments arranged by size:
1842 1455 627 505 499 325 217
BanII G_rGCy'C
Cuts at: 1391 2150 2472 2789 1391
Size: 759 322 317 4072
Fragments arranged by size:
4072 759 322 317
BbeI G_GCGC'C
Cuts at: 2290 2290
Size: 5470
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3661 3695 1903
Size: 34 1724 34 3678
Fragments arranged by size:
3678 1724 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3362 4572 3362
Size: 1210 4260
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5470
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4081 136
Size: 107 122 71 631 605 2409 1525
Fragments arranged by size:
2409 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5465 5465
Size: 5470
BlpI GC'TnA_GC
Cuts at: 2887 2887
Size: 5470
Bme1580I G_kGCm'C
Cuts at: 1790 3531 4687 1790
Size: 1741 1156 2573
Fragments arranged by size:
2573 1741 1156
BmgBI CAC'GTC
Cuts at: 1318 2011 1318
Size: 693 4777
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3201 4159 442
Size: 416 53 83 399 24 1784 958 1753
Fragments arranged by size:
1784 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2660 2682 2715 4130 1239
Size: 1421 22 33 1415 2579
Fragments arranged by size:
2579 1421 1415 33 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2306 3595 4460 4758 2306
Size: 1289 865 298 3018
Fragments arranged by size:
3018 1289 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4133 882
Size: 617 2634 2219
Fragments arranged by size:
2634 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2167 3261 493
Size: 711 963 1094 2702
Fragments arranged by size:
2702 1094 963 711
BsaBI GATnn'nnATC
Cuts at: 5464 5464
Size: 5470
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2287 3277 3659 4294
Size: 53 83 186 936 754 990 382 635
Cuts at: 4294 275
Size: 1451
Fragments arranged by size:
1451 990 936 754 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2640 3903 4301 4575 4722 5133 2640
Size: 1263 398 274 147 411 2977
Fragments arranged by size:
2977 1263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 1178
Size: 30 165 30 639 30 4576
Fragments arranged by size:
4576 639 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2641 1186
Size: 1455 4015
BseYI C'CCAG_C
Cuts at: 4673 5169 4673
Size: 496 4974
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4897
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2920 3681 4709 1540
Size: 275 186 919 761 1028 2301
Fragments arranged by size:
2301 1028 919 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2166 3531 3616 4687 2166
Size: 1365 85 1071 2949
Fragments arranged by size:
2949 1365 1071 85
BsmI GAATG_Cn'
Cuts at: 2386 2981 3074 2386
Size: 595 93 4782
Fragments arranged by size:
4782 595 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2166 2472 2789 3531 3616 4687
Size: 399 360 16 306 317 742 85 1071
Cuts at: 4687 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 360 317 306 85
16
BspEI T'CCGG_A
Cuts at: 5133 5133
Size: 5470
BspHI T'CATG_A
Cuts at: 3359 5089 3359
Size: 1730 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2416 2825 844
Size: 1572 409 3489
Fragments arranged by size:
3489 1572 409
BsrBI CCG'CTC
Cuts at: 1394 1823 2281 3357 1394
Size: 429 458 1076 3507
Fragments arranged by size:
3507 1076 458 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3965 4139 65
Size: 1183 2717 174 1396
Fragments arranged by size:
2717 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2180 4114 4301 1700
Size: 110 370 1934 187 2869
Fragments arranged by size:
2869 1934 370 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5470
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5470
BssSI C'ACGA_G
Cuts at: 3530 4542 3530
Size: 1012 4458
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2226 2413 794
Size: 1432 187 3851
Fragments arranged by size:
3851 1432 187
BstBI TT'CG_AA
Cuts at: 2313 4274 2313
Size: 1961 3509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5470
BstXI CCAn_nnnn'nTGG
Cuts at: 2195 2248 2635 2195
Size: 53 387 5030
Fragments arranged by size:
5030 387 53
BstYI r'GATC_y
Cuts at: 1972 2370 2680 2800 3167 3552 3569 5021 5107
Size: 398 310 120 367 385 17 1452 86
Cuts at: 5107 5119 5465 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 398 385 367 346 310 120
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2375 2375
Size: 5470
BtgI C'CryG_G
Cuts at: 513 1065 2004 2247 513
Size: 552 939 243 3736
Fragments arranged by size:
3736 939 552 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4731
Fragments arranged by size:
4731 683 56
BtsI GCAGTG_nn'
Cuts at: 2200 2986 3780 3800 2200
Size: 786 794 20 3870
Fragments arranged by size:
3870 794 786 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5435
DraI TTT'AAA
Cuts at: 2774 2774
Size: 5470
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4477 809
Size: 507 3161 1802
Fragments arranged by size:
3161 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2182 2917 3806
Size: 54 1425 50 275 18 352 735 889
Cuts at: 3806 5435 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 735 352 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2917 1537
Size: 275 1105 4090
Fragments arranged by size:
4090 1105 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3400 1353
Size: 2047 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2272 4057 4431 4577 387
Size: 1064 138 683 1785 374 146 1280
Fragments arranged by size:
1785 1280 1064 683 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2660 2682 2715 3533 4130 4917
Size: 393 680 348 22 33 818 597 787
Cuts at: 4917 5185 1239
Size: 268 1524
Fragments arranged by size:
1524 818 787 680 597 393 348 268
33 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2230 2294 2230
Size: 64 5406
EcoRI G'AATT_C
Cuts at: 2779 3157 2779
Size: 378 5092
FspI TGC'GCA
Cuts at: 3976 3976
Size: 5470
HaeII r_GCGC'y
Cuts at: 1678 2177 2290 4617 1678
Size: 499 113 2327 2531
Fragments arranged by size:
2531 2327 499 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2609 2617 2641 4111
Size: 32 135 32 1013 24 8 24 1470
Cuts at: 4111 4143 4185 4217 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1470 1013 135 42 32 32 32
32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2302 2814 3060 669
Size: 988 281 364 512 246 3079
Fragments arranged by size:
3079 988 512 364 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5470
HpaI GTT'AAC
Cuts at: 3060 3060
Size: 5470
KasI G'GCGC_C
Cuts at: 2286 2286
Size: 5470
KpnI G_GTAC'C
Cuts at: 2795 2795
Size: 5470
MfeI C'AATT_G
Cuts at: 3069 3069
Size: 5470
MluI A'CGCG_T
Cuts at: 5346 5346
Size: 5470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2511 2652 4584 4768 1305
Size: 1206 141 1932 184 2007
Fragments arranged by size:
2007 1932 1206 184 141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3648
Fragments arranged by size:
3648 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2246 3428 3787 3946 518
Size: 648 843 237 1182 359 159 2042
Fragments arranged by size:
2042 1182 843 648 359 237 159
MspA1I CmG'CkG
Cuts at: 1606 2886 3563 4711 4956 1606
Size: 1280 677 1148 245 2120
Fragments arranged by size:
2120 1280 1148 677 245
NarI GG'CG_CC
Cuts at: 2287 2287
Size: 5470
NcoI C'CATG_G
Cuts at: 513 1065 2004 2247 513
Size: 552 939 243 3736
Fragments arranged by size:
3736 939 552 243
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5470
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5470
NotI GC'GGCC_GC
Cuts at: 2917 2917
Size: 5470
NspI r_CATG'y
Cuts at: 1132 1229 2506 2828 4373 1132
Size: 97 1277 322 1545 2229
Fragments arranged by size:
2229 1545 1277 322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5470
PciI A'CATG_T
Cuts at: 4369 4369
Size: 5470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2683 1108
Size: 1575 3895
PfoI T'CCnGG_A
Cuts at: 2676 2676
Size: 5470
PmeI GTTT'AAAC
Cuts at: 2774 2774
Size: 5470
PmlI CAC'GTG
Cuts at: 2167 2167
Size: 5470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3539 3571 5052 5084 3539
Size: 32 1481 32 3925
Fragments arranged by size:
3925 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5470
PsiI TTA'TAA
Cuts at: 3040 3040
Size: 5470
PstI C_TGCA'G
Cuts at: 830 2145 2409 2822 830
Size: 1315 264 413 3478
Fragments arranged by size:
3478 1315 413 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5470
SalI G'TCGA_C
Cuts at: 1936 2812 1936
Size: 876 4594
SbfI CC_TGCA'GG
Cuts at: 2409 2822 2409
Size: 413 5057
ScaI AGT'ACT
Cuts at: 2045 2545 3718 5430 2045
Size: 500 1173 1712 2085
Fragments arranged by size:
2085 1712 1173 500
SfcI C'TryA_G
Cuts at: 826 1045 2141 2405 2440 2818 3953 4634 4825
Size: 219 1096 264 35 378 1135 681 191
Cuts at: 4825 826
Size: 1471
Fragments arranged by size:
1471 1135 1096 681 378 264 219 191
35
SfoI GGC'GCC
Cuts at: 2288 2288
Size: 5470
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5470
SmaI CCC'GGG
Cuts at: 1872 2797 1872
Size: 925 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2321 3574 4475 4737 820
Size: 197 926 378 1253 901 262 1553
Fragments arranged by size:
1553 1253 926 901 378 262 197
SnaBI TAC'GTA
Cuts at: 493 3261 493
Size: 2768 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5470
SphI G_CATG'C
Cuts at: 1132 2506 2828 1132
Size: 1374 322 3774
Fragments arranged by size:
3774 1374 322
SspI AAT'ATT
Cuts at: 5 52 3394 5
Size: 47 3342 2081
Fragments arranged by size:
3342 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5470
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 2361 2909 513
Size: 552 939 243 114 548 3074
Fragments arranged by size:
3074 939 552 548 243 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3497 3514 3667 3852 4297 325
Size: 1255 1917 17 153 185 445 1498
Fragments arranged by size:
1917 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2043 2543 3716
Size: 274 80 33 51 1443 66 500 1173
Cuts at: 3716 5428 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1173 500 274 138 80 66
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5430 5462 5430
Size: 32 5438
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5470
XbaI T'CTAG_A
Cuts at: 2806 2806
Size: 5470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2192 2011
Size: 181 5289
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5470
XmaI C'CCGG_G
Cuts at: 1870 2795 1870
Size: 925 4545
XmnI GAAnn'nnTTC
Cuts at: 3599 3599
Size: 5470
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3278 276
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI