################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI AlwNI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseRI BsiEI BsmI BspDI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MscI MunI NarI NcoI NheI NotI NruI NsiI NspI NspV PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 471 GAATTT [GA]^AATT[CT] AflIII 908 ACACGT A^C[GA][CT]GT Alw44I 1007 GTGCAC G^TGCAC AocI 58 CCTGAGG CC^T[ACGT]AGG ApaLI 1007 GTGCAC G^TGCAC ApoI 471 GAATTT [GA]^AATT[CT] Asp700I 87 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 935 CACGTG CAC^GTG BsaAI 935 CACGTG [CT]AC^GT[GA] BsgI 932 CTGCAC GTGCAG(16/14) BsiWI 41 CGTACG C^GTACG BsmBI 661 CGTCTC CGTCTC(1/5) Bsp1407I 410 TGTACA T^GTACA BsrBI 52 GAGCGG CCGCTC(-3/-3) BsrFI 636 GCCGGC [GA]^CCGG[CT] BsrGI 410 TGTACA T^GTACA BsrI 1023 CCAGT ACTGG(1/-1) BstF5I 233 GGATG GGATG(2/0) BstXI 98 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 746 GGATCT [GA]^GATC[CT] Bsu36I 58 CCTGAGG CC^T[ACGT]AGG Cfr10I 636 GCCGGC [GA]^CCGG[CT] DrdI 598 GACCAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 575 CGGCCA [CT]^GGCC[GA] Eco81I 58 CCTGAGG CC^T[ACGT]AGG EcoRV 485 GATATC GAT^ATC FokI 233 GGATG GGATG(9/13) MflI 746 GGATCT [GA]^GATC[CT] MslI 909 CACGTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 636 GCCGGC GCC^GGC NdeI 428 CATATG CA^TATG NgoMIV 636 GCCGGC G^CCGGC NlaIII 579 CATG CATG^ PacI 805 TTAATTAA TTAAT^TAA PflMI 444 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 935 CACGTG CAC^GTG PmlI 935 CACGTG CAC^GTG SexAI 104 ACCTGGT A^CC[AT]GGT SfaNI 535 GATGC GCATC(5/9) SmaI 979 CCCGGG CCC^GGG SspBI 410 TGTACA T^GTACA Van91I 444 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 746 GGATCT [GA]^GATC[CT] XmaCI 979 CCCGGG C^CCGGG XmaI 979 CCCGGG C^CCGGG XmnI 87 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 20 CGCG CG^CG 226 CGCG AccIII 194 TCCGGA T^CCGGA 374 TCCGGA AfaI 42 GTAC GT^AC 411 GTAC AlwI 32 GGATC GGATC(4/5) 746 GGATC Aor51HI 14 AGCGCT AGC^GCT 185 AGCGCT AspHI 214 GAGCAC G[AT]GC[AT]^C 1007 GTGCAC AvaI 610 CTCGGG C^[CT]CG[GA]G 979 CCCGGG BanI 434 GGCACC G^G[CT][GA]CC 1019 GGCACC BbsI 378 GAAGAC GAAGAC(2/6) 591 GAAGAC BpuAI 378 GAAGAC GAAGAC(2/6) 591 GAAGAC BseAI 194 TCCGGA T^CCGGA 374 TCCGGA BsiHKAI 214 GAGCAC G[AT]GC[AT]^C 1007 GTGCAC BsmAI 733 GAGAC GTCTC(1/5) 662 GTCTC BsoBI 610 CTCGGG C^[CT]CG[GA]G 979 CCCGGG BspEI 194 TCCGGA T^CCGGA 374 TCCGGA BspMI 971 ACCTGC ACCTGC(4/8) 918 GCAGGT BstUI 20 CGCG CG^CG 226 CGCG DsaI 383 CCACGG C^C[GA][CT]GG 728 CCGTGG Eco47III 14 AGCGCT AGC^GCT 185 AGCGCT HaeII 14 AGCGCT [GA]GCGC^[CT] 185 AGCGCT HphI 116 TCACC GGTGA(8/7) 969 TCACC MaeIII 109 GTCAC ^GT[ACGT]AC 905 GTCAC MroI 194 TCCGGA T^CCGGA 374 TCCGGA MspA1I 208 CCGCTG C[AC]G^C[GT]G 693 CCGCTG MvnI 20 CGCG CG^CG 226 CGCG PstI 148 CTGCAG CTGCA^G 973 CTGCAG RsaI 42 GTAC GT^AC 411 GTAC Tsp45I 109 GTCAC ^GT[GC]AC 905 GTCAC Tsp509I 472 AATT ^AATT 807 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcyI AluI ApaI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsaWI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIV PpuMI Sau3AI Sau96I ScrFI SfcI TaqI TfiI Tru9I TseI TspRI TthHB8I