(Circular) (Six-base) MAPSORT of: pFN21AE4064.seq Check: 7643 from: 1 to: 5737 pFN21AE4064 5737 bp With 182 enzymes: SgfI * March 28, 2012 13:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2914 2985 2914 Size: 71 5666 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3547 278 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3080 1937 Size: 1143 4594 Acc65I G'GTAC_C Cuts at: 3058 3058 Size: 5737 AclI AA'CG_TT Cuts at: 1563 3864 4237 1563 Size: 2301 373 3063 Fragments arranged by size: 3063 2301 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2615 3800 5184 5452 1632 Size: 983 1185 1384 268 1917 Fragments arranged by size: 1917 1384 1185 983 268 AfeI AGC'GCT Cuts at: 2021 2192 2021 Size: 171 5566 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5540 AflIII A'CryG_T Cuts at: 1203 2913 4636 5613 1203 Size: 1710 1723 977 1327 Fragments arranged by size: 1723 1710 1327 977 AgeI A'CCGG_T Cuts at: 4568 4568 Size: 5737 AhdI GACnn_n'nnGTC Cuts at: 4466 4466 Size: 5737 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5737 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5737 AlwNI CAG_nnn'CTG Cuts at: 3480 5052 3480 Size: 1572 4165 ApaI G_GGCC'C Cuts at: 2257 2656 2968 2257 Size: 399 312 5026 Fragments arranged by size: 5026 399 312 ApaLI G'TGCA_C Cuts at: 3012 3794 4950 3012 Size: 782 1156 3799 Fragments arranged by size: 3799 1156 782 ApoI r'AATT_y Cuts at: 1493 2476 3046 3272 3424 5533 1493 Size: 983 570 226 152 2109 1697 Fragments arranged by size: 2109 1697 983 570 226 152 AseI AT'TA_AT Cuts at: 160 1032 4291 160 Size: 872 3259 1606 Fragments arranged by size: 3259 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 AvaI C'yCGr_G Cuts at: 1870 1943 2615 2984 3062 1870 Size: 73 672 369 78 4545 Fragments arranged by size: 4545 672 369 78 73 BamHI G'GATC_C Cuts at: 3067 3434 3067 Size: 367 5370 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2439 3024 3058 4513 618 Size: 325 217 627 652 585 34 1455 1842 Fragments arranged by size: 1842 1455 652 627 585 325 217 34 BanII G_rGCy'C Cuts at: 1391 2257 2656 2968 3056 1391 Size: 866 399 312 88 4072 Fragments arranged by size: 4072 866 399 312 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2390 2603 928 Size: 971 491 213 4062 Fragments arranged by size: 4062 971 491 213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3928 3962 1903 Size: 34 50 34 1907 34 3678 Fragments arranged by size: 3678 1907 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3629 4839 3629 Size: 1210 4527 BclI T'GATC_A Cuts at: 1548 1548 Size: 5737 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4348 136 Size: 107 122 71 631 605 2676 1525 Fragments arranged by size: 2676 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5732 5732 Size: 5737 BlpI GC'TnA_GC Cuts at: 3154 3154 Size: 5737 Bme1580I G_kGCm'C Cuts at: 1790 2230 2257 2442 2656 2968 3016 3027 3798 Size: 440 27 185 214 312 48 11 771 Cuts at: 3798 4954 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 771 440 312 214 185 48 27 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5737 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3468 4426 442 Size: 416 53 83 399 24 2051 958 1753 Fragments arranged by size: 2051 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2086 2237 2285 2345 4397 1239 Size: 847 151 48 60 2052 2579 Fragments arranged by size: 2579 2052 847 151 60 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3862 4727 5025 3862 Size: 865 298 4574 Fragments arranged by size: 4574 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4400 882 Size: 617 2901 2219 Fragments arranged by size: 2901 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2942 3528 493 Size: 711 1738 586 2702 Fragments arranged by size: 2702 1738 711 586 BsaBI GATnn'nnATC Cuts at: 5731 5731 Size: 5737 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 2166 2331 3544 Size: 53 83 186 936 546 87 165 1213 Cuts at: 3544 3926 4561 275 Size: 382 635 1451 Fragments arranged by size: 1451 1213 936 635 546 382 186 165 87 83 53 BsaWI w'CCGG_w Cuts at: 2199 2352 2379 4170 4568 4842 4989 5400 2199 Size: 153 27 1791 398 274 147 411 2536 Fragments arranged by size: 2536 1791 411 398 274 153 147 27 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5512 Fragments arranged by size: 5512 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5737 BseYI C'CCAG_C Cuts at: 2587 4940 5436 2587 Size: 2353 496 2888 Fragments arranged by size: 2888 2353 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2922 1163 Size: 573 1186 3978 Fragments arranged by size: 3978 1186 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3187 3948 4976 1540 Size: 275 186 1186 761 1028 2301 Fragments arranged by size: 2301 1186 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 3016 3798 3883 4954 2223 Size: 793 782 85 1071 3006 Fragments arranged by size: 3006 1071 793 782 85 BsiWI C'GTAC_G Cuts at: 2046 2046 Size: 5737 BsmI GAATG_Cn' Cuts at: 3248 3341 3248 Size: 93 5644 BsmBI CGTCTCn'nnnn_ Cuts at: 2672 2672 Size: 5737 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2230 2257 2442 2656 2968 3016 Size: 399 433 7 27 185 214 312 48 Cuts at: 3016 3027 3056 3798 3883 4954 1391 Size: 11 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 433 399 312 214 185 85 48 29 27 11 7 BspEI T'CCGG_A Cuts at: 2199 2379 5400 2199 Size: 180 3021 2536 Fragments arranged by size: 3021 2536 180 BspHI T'CATG_A Cuts at: 3626 5356 3626 Size: 1730 4007 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2914 2985 3092 844 Size: 2070 71 107 3489 Fragments arranged by size: 3489 2070 107 71 BsrBI CCG'CTC Cuts at: 1394 1823 2059 3624 1394 Size: 429 236 1565 3507 Fragments arranged by size: 3507 1565 429 236 BsrDI GCAATG_nn' Cuts at: 65 1248 4232 4406 65 Size: 1183 2984 174 1396 Fragments arranged by size: 2984 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2641 4381 4568 1700 Size: 110 831 1740 187 2869 Fragments arranged by size: 2869 1740 831 187 110 BsrGI T'GTAC_A Cuts at: 96 2415 96 Size: 2319 3418 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5737 BssSI C'ACGA_G Cuts at: 3797 4809 3797 Size: 1012 4725 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5737 BstBI TT'CG_AA Cuts at: 4541 4541 Size: 5737 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5737 BstXI CCAn_nnnn'nTGG Cuts at: 2110 2110 Size: 5737 BstYI r'GATC_y Cuts at: 1972 2751 3067 3434 3819 3836 5288 5374 5386 Size: 779 316 367 385 17 1452 86 12 Cuts at: 5386 5732 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 779 385 367 346 316 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2064 2064 Size: 5737 BtgI C'CryG_G Cuts at: 513 1065 2388 2733 513 Size: 552 1323 345 3517 Fragments arranged by size: 3517 1323 552 345 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4998 Fragments arranged by size: 4998 683 56 BtsI GCAGTG_nn' Cuts at: 3253 4047 4067 3253 Size: 794 20 4923 Fragments arranged by size: 4923 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5737 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5702 DraI TTT'AAA Cuts at: 3041 3041 Size: 5737 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2609 4744 809 Size: 507 1293 2135 1802 Fragments arranged by size: 2135 1802 1293 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2580 3184 4073 Size: 54 1425 50 275 18 750 604 889 Cuts at: 4073 5702 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 750 604 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3184 1537 Size: 275 1372 4090 Fragments arranged by size: 4090 1372 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3667 1353 Size: 2314 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4324 4698 4844 387 Size: 1064 138 2735 374 146 1280 Fragments arranged by size: 2735 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 2237 2285 2345 2615 3800 4397 Size: 393 454 151 48 60 270 1185 597 Cuts at: 4397 5184 5452 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1185 787 597 454 393 270 268 151 60 48 EcoO109I rG'GnC_Cy Cuts at: 2029 2209 2253 2409 2611 2652 2653 2964 2965 Size: 180 44 156 202 41 1 311 1 Cuts at: 2965 2029 Size: 4801 Fragments arranged by size: 4801 311 202 180 156 44 41 1 1 EcoRI G'AATT_C Cuts at: 3046 3424 3046 Size: 378 5359 EcoRV GAT'ATC Cuts at: 2492 2492 Size: 5737 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2806 2806 Size: 5737 FspI TGC'GCA Cuts at: 4243 4243 Size: 5737 HaeII r_GCGC'y Cuts at: 1678 2023 2194 4884 1678 Size: 345 171 2690 2531 Fragments arranged by size: 2690 2531 345 171 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2650 2682 2893 2925 4378 Size: 32 135 32 1078 32 211 32 1453 Cuts at: 4378 4410 4452 4484 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1453 1078 211 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3081 3327 669 Size: 988 281 1143 246 3079 Fragments arranged by size: 3079 1143 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5737 HpaI GTT'AAC Cuts at: 3327 3327 Size: 5737 KpnI G_GTAC'C Cuts at: 3062 3062 Size: 5737 MfeI C'AATT_G Cuts at: 3336 3336 Size: 5737 MluI A'CGCG_T Cuts at: 5613 5613 Size: 5737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2530 4851 5035 1305 Size: 1225 2321 184 2007 Fragments arranged by size: 2321 2007 1225 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3915 Fragments arranged by size: 3915 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2918 3695 4054 4213 518 Size: 648 1752 777 359 159 2042 Fragments arranged by size: 2042 1752 777 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2215 2700 3153 3830 4978 5223 1606 Size: 609 485 453 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 609 485 453 245 NaeI GCC'GGC Cuts at: 2643 2643 Size: 5737 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5185 NdeI CA'TA_TG Cuts at: 387 2434 387 Size: 2047 3690 NgoMIV G'CCGG_C Cuts at: 2641 2641 Size: 5737 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5737 NotI GC'GGCC_GC Cuts at: 3184 3184 Size: 5737 NspI r_CATG'y Cuts at: 1132 1229 3095 4640 1132 Size: 97 1866 1545 2229 Fragments arranged by size: 2229 1866 1545 97 PacI TTA_AT'TAA Cuts at: 2814 2814 Size: 5737 PasI CC'CwG_GG Cuts at: 1348 2959 1348 Size: 1611 4126 PciI A'CATG_T Cuts at: 4636 4636 Size: 5737 PflMI CCAn_nnn'nTGG Cuts at: 1108 2455 1108 Size: 1347 4390 PmeI GTTT'AAAC Cuts at: 3041 3041 Size: 5737 PmlI CAC'GTG Cuts at: 2942 2942 Size: 5737 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3806 3838 5319 5351 3806 Size: 32 1481 32 4192 Fragments arranged by size: 4192 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2029 2209 2409 2611 2029 Size: 180 200 202 5155 Fragments arranged by size: 5155 202 200 180 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5737 PsiI TTA'TAA Cuts at: 2504 3307 2504 Size: 803 4934 PspOMI G'GGCC_C Cuts at: 2253 2652 2964 2253 Size: 399 312 5026 Fragments arranged by size: 5026 399 312 PstI C_TGCA'G Cuts at: 830 2157 2982 3089 830 Size: 1327 825 107 3478 Fragments arranged by size: 3478 1327 825 107 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5737 SalI G'TCGA_C Cuts at: 1936 3079 1936 Size: 1143 4594 SanDI GG'GwC_CC Cuts at: 2029 2209 2029 Size: 180 5557 SbfI CC_TGCA'GG Cuts at: 3089 3089 Size: 5737 ScaI AGT'ACT Cuts at: 3985 5697 3985 Size: 1712 4025 SexAI A'CCwGG_T Cuts at: 2109 2109 Size: 5737 SfcI C'TryA_G Cuts at: 826 1045 2153 2572 2978 3085 4220 4901 5092 Size: 219 1108 419 406 107 1135 681 191 Cuts at: 5092 826 Size: 1471 Fragments arranged by size: 1471 1135 1108 681 419 406 219 191 107 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5737 SmaI CCC'GGG Cuts at: 1872 2986 3064 1872 Size: 1114 78 4545 Fragments arranged by size: 4545 1114 78 SmlI C'TyrA_G Cuts at: 820 1017 1943 3841 4742 5004 820 Size: 197 926 1898 901 262 1553 Fragments arranged by size: 1898 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3528 493 Size: 3035 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5737 SphI G_CATG'C Cuts at: 1132 3095 1132 Size: 1963 3774 SspI AAT'ATT Cuts at: 5 52 3661 5 Size: 47 3609 2081 Fragments arranged by size: 3609 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5737 StyI C'CwwG_G Cuts at: 513 1065 3176 513 Size: 552 2111 3074 Fragments arranged by size: 3074 2111 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 3764 3781 3934 4119 4564 325 Size: 1255 1035 1149 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1149 1035 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2415 3983 5695 Size: 274 80 33 51 1443 438 1568 1712 Cuts at: 5695 96 Size: 138 Fragments arranged by size: 1712 1568 1443 438 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2836 2868 5697 5729 2836 Size: 32 2829 32 2844 Fragments arranged by size: 2844 2829 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5737 XbaI T'CTAG_A Cuts at: 3073 3073 Size: 5737 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5737 XmaI C'CCGG_G Cuts at: 1870 2984 3062 1870 Size: 1114 78 4545 Fragments arranged by size: 4545 1114 78 XmnI GAAnn'nnTTC Cuts at: 2096 3866 2096 Size: 1770 3967 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3545 276 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NarI NruI NsiI PfoI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI