Restriction map for
pFN21AE4064
(Linear) (Six-base) MAPSORT of: KIEE4064.seq Check: 6931 from: 1 to: 1032
KIEE4064.orf
With 182 enzymes: SgfI *
March 22, 2012 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 909 980 1032
Size: 909 71 52
Fragments arranged by size:
909 71 52
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 1032
Size: 610 422
AfeI AGC'GCT
Cuts at: 0 16 187 1032
Size: 16 171 845
Fragments arranged by size:
845 171 16
AflIII A'CryG_T
Cuts at: 0 908 1032
Size: 908 124
ApaI G_GGCC'C
Cuts at: 0 252 651 963 1032
Size: 252 399 312 69
Fragments arranged by size:
399 312 252 69
ApaLI G'TGCA_C
Cuts at: 0 1007 1032
Size: 1007 25
ApoI r'AATT_y
Cuts at: 0 471 1032
Size: 471 561
AvaI C'yCGr_G
Cuts at: 0 610 979 1032
Size: 610 369 53
Fragments arranged by size:
610 369 53
BanI G'GyrC_C
Cuts at: 0 434 1019 1032
Size: 434 585 13
Fragments arranged by size:
585 434 13
BanII G_rGCy'C
Cuts at: 0 252 651 963 1032
Size: 252 399 312 69
Fragments arranged by size:
399 312 252 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 385 598 1032
Size: 385 213 434
Fragments arranged by size:
434 385 213
Bme1580I G_kGCm'C
Cuts at: 0 225 252 437 651 963 1011 1022 1032
Size: 225 27 185 214 312 48 11 10
Fragments arranged by size:
312 225 214 185 48 27 11 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 232 280 340 1032
Size: 81 151 48 60 692
Fragments arranged by size:
692 151 81 60 48
BsaAI yAC'GTr
Cuts at: 0 937 1032
Size: 937 95
BsaHI Gr'CG_yC
Cuts at: 0 74 161 326 1032
Size: 74 87 165 706
Fragments arranged by size:
706 165 87 74
BsaWI w'CCGG_w
Cuts at: 0 194 347 374 1032
Size: 194 153 27 658
Fragments arranged by size:
658 194 153 27
BseYI C'CCAG_C
Cuts at: 0 582 1032
Size: 582 450
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 1032
Size: 917 115
BsiHKAI G_wGCw'C
Cuts at: 0 218 1011 1032
Size: 218 793 21
Fragments arranged by size:
793 218 21
BsiWI C'GTAC_G
Cuts at: 0 41 1032
Size: 41 991
BsmBI CGTCTCn'nnnn_
Cuts at: 0 667 1032
Size: 667 365
Bsp1286I G_dGCh'C
Cuts at: 0 218 225 252 437 651 963 1011 1022
Size: 218 7 27 185 214 312 48 11
Cuts at: 1022 1032
Size: 10
Fragments arranged by size:
312 218 214 185 48 27 11 10
7
BspEI T'CCGG_A
Cuts at: 0 194 374 1032
Size: 194 180 658
Fragments arranged by size:
658 194 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 909 980 1032
Size: 909 71 52
Fragments arranged by size:
909 71 52
BsrBI CCG'CTC
Cuts at: 0 54 1032
Size: 54 978
BsrFI r'CCGG_y
Cuts at: 0 636 1032
Size: 636 396
BsrGI T'GTAC_A
Cuts at: 0 410 1032
Size: 410 622
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 1032
Size: 105 927
BstYI r'GATC_y
Cuts at: 0 746 1032
Size: 746 286
Bsu36I CC'TnA_GG
Cuts at: 0 59 1032
Size: 59 973
BtgI C'CryG_G
Cuts at: 0 383 728 1032
Size: 383 345 304
Fragments arranged by size:
383 345 304
DrdI GACnn_nn'nnGTC
Cuts at: 0 604 1032
Size: 604 428
EaeI y'GGCC_r
Cuts at: 0 575 1032
Size: 575 457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 232 280 340 610 1032
Size: 81 151 48 60 270 422
Fragments arranged by size:
422 270 151 81 60 48
EcoO109I rG'GnC_Cy
Cuts at: 0 24 204 248 404 606 647 648 959
Size: 24 180 44 156 202 41 1 311
Cuts at: 959 960 1032
Size: 1 72
Fragments arranged by size:
311 202 180 156 72 44 41 24
1 1
EcoRV GAT'ATC
Cuts at: 0 487 1032
Size: 487 545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 801 1032
Size: 801 231
HaeII r_GCGC'y
Cuts at: 0 18 189 1032
Size: 18 171 843
Fragments arranged by size:
843 171 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 645 677 888 920 1032
Size: 645 32 211 32 112
Fragments arranged by size:
645 211 112 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1032
Size: 525 507
MslI CAynn'nnrTG
Cuts at: 0 913 1032
Size: 913 119
MspA1I CmG'CkG
Cuts at: 0 210 695 1032
Size: 210 485 337
Fragments arranged by size:
485 337 210
NaeI GCC'GGC
Cuts at: 0 638 1032
Size: 638 394
NdeI CA'TA_TG
Cuts at: 0 429 1032
Size: 429 603
NgoMIV G'CCGG_C
Cuts at: 0 636 1032
Size: 636 396
PacI TTA_AT'TAA
Cuts at: 0 809 1032
Size: 809 223
PasI CC'CwG_GG
Cuts at: 0 954 1032
Size: 954 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 450 1032
Size: 450 582
PmlI CAC'GTG
Cuts at: 0 937 1032
Size: 937 95
PpuMI rG'GwC_Cy
Cuts at: 0 24 204 404 606 1032
Size: 24 180 200 202 426
Fragments arranged by size:
426 202 200 180 24
PsiI TTA'TAA
Cuts at: 0 499 1032
Size: 499 533
PspOMI G'GGCC_C
Cuts at: 0 248 647 959 1032
Size: 248 399 312 73
Fragments arranged by size:
399 312 248 73
PstI C_TGCA'G
Cuts at: 0 152 977 1032
Size: 152 825 55
Fragments arranged by size:
825 152 55
SanDI GG'GwC_CC
Cuts at: 0 24 204 1032
Size: 24 180 828
Fragments arranged by size:
828 180 24
SexAI A'CCwGG_T
Cuts at: 0 104 1032
Size: 104 928
SfcI C'TryA_G
Cuts at: 0 148 567 973 1032
Size: 148 419 406 59
Fragments arranged by size:
419 406 148 59
SmaI CCC'GGG
Cuts at: 0 981 1032
Size: 981 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 1032
Size: 610 422
TatI w'GTAC_w
Cuts at: 0 410 1032
Size: 410 622
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 831 863 1032
Size: 831 32 169
Fragments arranged by size:
831 169 32
XmaI C'CCGG_G
Cuts at: 0 979 1032
Size: 979 53
XmnI GAAnn'nnTTC
Cuts at: 0 91 1032
Size: 91 941
Enzymes that do cut:
AarI AcuI AfeI AflIII ApaI ApaLI ApoI AvaI
BanI BanII BbsI Bme1580I BpmI BsaAI BsaHI BsaWI
BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BstXI BstYI Bsu36I BtgI DrdI
EaeI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I MmeI
MslI MspA1I NaeI NdeI NgoMIV PacI PasI PflMI
PmlI PpuMI PsiI PspOMI PstI SanDI SexAI SfcI
SmaI TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaXI BseRI BsiEI BsmI BspHI BsrDI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI
EcoNI EcoRI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NarI NcoI NheI
NotI NruI NsiI NspI PciI PfoI PmeI PpiI
PshAI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI ZraI