Restriction map for
pFN21AB6760
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT22I FauI FbaI
FseI FspI HapII HgaI Hin1I
HindIII HinfI HpaI HpaII KasI
KpnI Ksp632I KspI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tsp45I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 215 AAATTT [GA]^AATT[CT]
AfaI 399 GTAC GT^AC
ApaI 114 GGGCCC GGGCC^C
ApoI 215 AAATTT [GA]^AATT[CT]
AspI 291 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 70 GGACC G^G[AT]CC
BanI 357 GGTGCC G^G[CT][GA]CC
BanII 114 GGGCCC G[GA]GC[CT]^C
BfaI 409 CTAG C^TAG
BmyI 114 GGGCCC G[AGT]GC[ACT]^C
BpmI 281 CTCCAG CTGGAG(16/14)
Bsp1286I 114 GGGCCC G[AGT]GC[ACT]^C
BsrDI 239 GCAATG GCAATG(2/0)
BssKI 354 CCTGG ^CC[ACGT]GG
BstAPI 245 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 159 CATCC GGATG(2/0)
BstNI 354 CCTGG CC^[AT]GG
BstXI 104 CCACCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 4 GCGC GCG^C
DdeI 272 CTCAG C^T[ACGT]AG
DraII 69 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 130 CCTTACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 69 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 354 CCTGG ^CC[AT]GG
EcoRV 259 GATATC GAT^ATC
EcoT14I 135 CCAAGG C^C[AT][AT]GG
FokI 159 CATCC GGATG(9/13)
HaeII 3 GGCGCT [GA]GCGC^[CT]
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
HincII 288 GTTGAC GT[CT]^[GA]AC
HindII 288 GTTGAC GT[CT]^[GA]AC
MaeI 409 CTAG C^TAG
MboII 149 TCTTC GAAGA(8/7)
MseI 28 TTAA T^TAA
MslI 105 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 354 CCTGG CC^[AT]GG
NlaIII 111 CATG CATG^
PflFI 291 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 69 AGGACCT [GA]G^G[AT]CC[CT]
PstI 65 CTGCAG CTGCA^G
RsaI 399 GTAC GT^AC
ScrFI 354 CCTGG CC^[ACGT]GG
SfaNI 248 GCATC GCATC(5/9)
StyI 135 CCAAGG C^C[AT][AT]GG
Tru9I 28 TTAA T^TAA
Tth111I 291 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 12 GCGG CCGC(-3/-1)
267 GCGG
AlwNI 68 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
246 CAGCATCTG
BamHI 14 GGATCC G^GATCC
45 GGATCC
BbvI 245 GCAGC GCAGC(8/12)
324 GCTGC
BsaI 269 GGTCTC GGTCTC(1/5)
278 GGTCTC
BsaJI 135 CCAAGG C^C[ACGT][ACGT]GG
353 CCCTGG
BsiYI 93 CCACTGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
130 CCTTACCAAGG
BslI 93 CCACTGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
130 CCTTACCAAGG
BsmAI 270 GTCTC GTCTC(1/5)
279 GTCTC
BsrI 95 ACTGG ACTGG(1/-1)
57 CCAGT
BstYI 14 GGATCC [GA]^GATC[CT]
45 GGATCC
Cac8I 99 GCAGGC GC[ACGT]^[ACGT]GC
320 GCTCGC
DpnII 15 GATC ^GATC
46 GATC
Fnu4HI 245 GCAGC GC^[ACGT]GC
324 GCTGC
HphI 79 GGTGA GGTGA(8/7)
222 TCACC
ItaI 245 GCAGC GC^[ACGT]GC
324 GCTGC
MboI 15 GATC ^GATC
46 GATC
MflI 14 GGATCC [GA]^GATC[CT]
45 GGATCC
MwoI 239 GCAATGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
245 GCAGCATCTGC
NdeII 15 GATC ^GATC
46 GATC
Sau3AI 15 GATC ^GATC
46 GATC
SfcI 65 CTGCAG C^T[GA][CT]AG
170 CTACAG
TseI 245 GCAGC G^C[AT]GC
324 GCTGC
Tsp509I 26 AATT ^AATT
216 AATT
XhoII 14 GGATCC [GA]^GATC[CT]
45 GGATCC
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
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AlwI Cfr13I HaeIII MnlI NlaIV
Sau96I TspRI