Restriction map for
pFN21AE0895
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AhdI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BamHI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BseAI BsgI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HpaI KpnI KspI
MaeI MaeII MfeI MluI MroI
MseI MspA1I MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TaqI Tru9I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AgeI 362 ACCGGT A^CCGGT
Alw44I 564 GTGCAC G^TGCAC
ApaLI 564 GTGCAC G^TGCAC
AspHI 564 GTGCAC G[AT]GC[AT]^C
AspI 331 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 896 GGGCTC G[GA]GC[CT]^C
BbeI 436 GGCGCC GGCGC^C
BcgI 556 CGACTTTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 260 CCGGG CC^[GC]GG
BsaBI 588 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 884 GAGACC GGTCTC(1/5)
BsaWI 362 ACCGGT [AT]^CCGG[AT]
BseRI 786 CTCCTC GAGGAG(10/8)
BsiHKAI 564 GTGCAC G[AT]GC[AT]^C
Bsp1407I 830 TGTACA T^GTACA
BsrFI 362 ACCGGT [GA]^CCGG[CT]
BsrGI 830 TGTACA T^GTACA
BsrI 20 CCAGT ACTGG(1/-1)
BstXI 312 CCATTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 587 GGATCT [GA]^GATC[CT]
CfoI 437 GCGC GCG^C
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
DraII 680 AGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 748 CTCTTC CTCTTC(1/4)
EcoO109I 680 AGGTCCC [GA]G^G[ACGT]CC[CT]
HaeII 436 GGCGCC [GA]GCGC^[CT]
HhaI 437 GCGC GCG^C
HinP1I 437 GCGC G^CGC
HincII 913 GTCAAC GT[CT]^[GA]AC
HindII 913 GTCAAC GT[CT]^[GA]AC
HindIII 815 AAGCTT A^AGCTT
KasI 436 GGCGCC G^GCGCC
Ksp632I 748 CTCTTC CTCTTC(1/4)
MaeIII 841 GTCAC ^GT[ACGT]AC
MamI 588 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 587 GGATCT [GA]^GATC[CT]
NarI 436 GGCGCC GG^CGCC
NciI 260 CCGGG CC^[GC]GG
NspI 98 GCATGT [GA]CATG^[CT]
PflFI 331 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 362 ACCGGT A^CCGGT
PpuMI 680 AGGTCCC [GA]G^G[AT]CC[CT]
SexAI 104 ACCTGGT A^CC[AT]GGT
SfoI 436 GGCGCC GGC^GCC
SmlI 873 CTTGAG C^T[CT][GA]AG
SspBI 830 TGTACA T^GTACA
TfiI 855 GAATC G^A[AT]TC
Tsp45I 841 GTCAC ^GT[GC]AC
Tsp509I 32 AATT ^AATT
Tth111I 331 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 191 CCAACCTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 587 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 436 GGCGCC G[GA]^CG[CT]C
769 GGCGTC
AlwI 294 GATCC GGATC(4/5)
587 GGATC
Asp700I 705 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
855 GAATCCCTTC
BanI 436 GGCGCC G^G[CT][GA]CC
519 GGTGCC
BglI 346 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
439 GCCCTCTGGGC
BmyI 564 GTGCAC G[AGT]GC[ACT]^C
896 GGGCTC
BsaHI 436 GGCGCC G[GA]^CG[CT]C
769 GGCGTC
BsmBI 493 CGTCTC CGTCTC(1/5)
70 GAGACG
Bsp1286I 564 GTGCAC G[AGT]GC[ACT]^C
896 GGGCTC
BspHI 404 TCATGA T^CATGA
674 TCATGA
BspMI 125 ACCTGC ACCTGC(4/8)
718 ACCTGC
HapII 260 CCGG C^CGG
363 CCGG
Hin1I 436 GGCGCC G[GA]^CG[CT]C
769 GGCGTC
HpaII 260 CCGG C^CGG
363 CCGG
MspI 260 CCGG C^CGG
363 CCGG
PleI 47 GACTC GAGTC(4/5)
154 GACTC
PstI 689 CTGCAG CTGCA^G
720 CTGCAG
RcaI 404 TCATGA T^CATGA
674 TCATGA
TspRI 21 CAGTG CA[GC]TG[ACGT][ACGT]^
801 CACTG
XmnI 705 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
855 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AvaII BalI
BbvI BsaJI BsiYI BslI BsmAI
BsmFI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HgaI HinfI
HphI ItaI MboI MboII MluNI
MnlI MscI MslI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI