Restriction map for
pFN21AE0891
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvaII
AvrII BamHI BbrPI BcgI BciVI
BclI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 775 GTCTAC GT^[AC][GT]AC
AcyI 610 GGCGCC G[GA]^CG[CT]C
AflII 555 CTTAAG C^TTAAG
AhdI 54 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 929 GGATC GGATC(4/5)
AspEI 54 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 413 CCCGGG C^[CT]CG[GA]G
AviII 424 TGCGCA TGC^GCA
BanII 908 GAGCCC G[GA]GC[CT]^C
BbeI 610 GGCGCC GGCGC^C
BbvCI 689 CCTCAGC CCTCAGC(-5/-2)
BfaI 938 CTAG C^TAG
BfrI 555 CTTAAG C^TTAAG
BglI 145 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 679 CTCCAG CTGGAG(16/14)
BsaAI 370 TACGTG [CT]AC^GT[GA]
BsaHI 610 GGCGCC G[GA]^CG[CT]C
BsoBI 413 CCCGGG C^[CT]CG[GA]G
BsrDI 768 CATTGC GCAATG(2/0)
BsrI 155 CCAGT ACTGG(1/-1)
BstYI 929 GGATCT [GA]^GATC[CT]
DpnII 930 GATC ^GATC
DraIII 517 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 353 CCATGG C^C[GA][CT]GG
Eam1105I 54 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 751 CTCTTC CTCTTC(1/4)
EcoNI 695 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 353 CCATGG C^C[AT][AT]GG
FspI 424 TGCGCA TGC^GCA
HaeII 610 GGCGCC [GA]GCGC^[CT]
HgaI 797 GACGC GACGC(5/10)
Hin1I 610 GGCGCC G[GA]^CG[CT]C
KasI 610 GGCGCC G^GCGCC
Ksp632I 751 CTCTTC CTCTTC(1/4)
MaeI 938 CTAG C^TAG
MboI 930 GATC ^GATC
MflI 929 GGATCT [GA]^GATC[CT]
MseI 556 TTAA T^TAA
NarI 610 GGCGCC GG^CGCC
NcoI 353 CCATGG C^CATGG
NdeII 930 GATC ^GATC
NruI 885 TCGCGA TCG^CGA
Sau3AI 930 GATC ^GATC
SfoI 610 GGCGCC GGC^GCC
SmaI 413 CCCGGG CCC^GGG
SmlI 555 CTTAAG C^T[CT][GA]AG
StyI 353 CCATGG C^C[AT][AT]GG
Tru9I 556 TTAA T^TAA
XhoII 929 GGATCT [GA]^GATC[CT]
XmaCI 413 CCCGGG C^CCGGG
XmaI 413 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 799 CGCG CG^CG
886 CGCG
AlwNI 692 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
873 CAGCCTCTG
AspHI 567 GTGCTC G[AT]GC[AT]^C
676 GTGCTC
BanI 383 GGCACC G^G[CT][GA]CC
610 GGCGCC
BbsI 18 GTCTTC GAAGAC(2/6)
341 GTCTTC
BpuAI 18 GTCTTC GAAGAC(2/6)
341 GTCTTC
BsiHKAI 567 GTGCTC G[AT]GC[AT]^C
676 GTGCTC
Bsp1407I 833 TGTACA T^GTACA
891 TGTACA
BsrGI 833 TGTACA T^GTACA
891 TGTACA
BssSI 402 CACGAG CACGAG(-5/-1)
564 CTCGTG
BstUI 799 CGCG CG^CG
886 CGCG
CfoI 425 GCGC GCG^C
611 GCGC
DdeI 690 CTCAG C^T[ACGT]AG
933 CTCAG
HhaI 425 GCGC GCG^C
611 GCGC
HinP1I 425 GCGC G^CGC
611 GCGC
MaeII 371 ACGT A^CGT
461 ACGT
MvnI 799 CGCG CG^CG
886 CGCG
SfcI 839 CTGTAG C^T[GA][CT]AG
870 CTACAG
SspBI 833 TGTACA T^GTACA
891 TGTACA
TfiI 29 GAATC G^A[AT]TC
882 GAATC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI BalI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstXI Cac8I Cfr13I EaeI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PleI PvuII RsaI Sau96I
ScrFI TseI Tsp45I TspRI