(Circular) (Six-base) MAPSORT of: pFN21AE0891.seq Check: 1828 from: 1 to: 5653 pFN21AE0891 5653 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2781 2996 1937 Size: 844 215 4594 Fragments arranged by size: 4594 844 215 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2010 3716 5100 5368 1632 Size: 378 1706 1384 268 1917 Fragments arranged by size: 1917 1706 1384 378 268 AflII C'TTAA_G Cuts at: 820 1017 2560 820 Size: 197 1543 3913 Fragments arranged by size: 3913 1543 197 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 2064 4382 2064 Size: 2318 3335 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 2702 2883 3396 4968 2702 Size: 181 513 1572 3387 Fragments arranged by size: 3387 1572 513 181 ApaLI G'TGCA_C Cuts at: 3710 4866 3710 Size: 1156 4497 ApoI r'AATT_y Cuts at: 1493 2962 3188 3340 5449 1493 Size: 1469 226 152 2109 1697 Fragments arranged by size: 2109 1697 1469 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2418 2978 1870 Size: 73 475 560 4545 Fragments arranged by size: 4545 560 475 73 BamHI G'GATC_C Cuts at: 2983 3350 2983 Size: 367 5286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2388 2615 2974 4429 618 Size: 325 217 627 601 227 359 1455 1842 Fragments arranged by size: 1842 1455 627 601 359 325 227 217 BanII G_rGCy'C Cuts at: 1391 2917 2972 1391 Size: 1526 55 4072 Fragments arranged by size: 4072 1526 55 BbeI G_GCGC'C Cuts at: 2619 2619 Size: 5653 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2016 2339 928 Size: 971 117 323 4242 Fragments arranged by size: 4242 971 323 117 BbvCI CC'TCA_GC Cuts at: 2695 2695 Size: 5653 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2156 4264 Size: 107 122 71 631 605 337 147 2108 Cuts at: 4264 136 Size: 1525 Fragments arranged by size: 2108 1525 631 605 337 147 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2244 2532 2908 3714 4870 1790 Size: 454 288 376 806 1156 2573 Fragments arranged by size: 2573 1156 806 454 376 288 BmgBI CAC'GTC Cuts at: 1318 2467 1318 Size: 1149 4504 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3384 4342 442 Size: 416 53 83 399 24 1967 958 1753 Fragments arranged by size: 1967 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2053 2936 2053 Size: 883 4770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 4313 1239 Size: 1430 1644 2579 Fragments arranged by size: 2579 1644 1430 Bpu10I CC'TnA_GC Cuts at: 2695 2695 Size: 5653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3778 4643 4941 3778 Size: 865 298 4490 Fragments arranged by size: 4490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2377 3444 493 Size: 711 1173 1067 2702 Fragments arranged by size: 2702 1173 1067 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2616 3460 3842 4477 Size: 53 83 186 936 1083 844 382 635 Cuts at: 4477 275 Size: 1451 Fragments arranged by size: 1451 1083 936 844 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4086 4484 4758 4905 5316 4086 Size: 398 274 147 411 4423 Fragments arranged by size: 4423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2456 2486 1178 Size: 30 165 30 1053 30 4345 Fragments arranged by size: 4345 1053 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5653 BseYI C'CCAG_C Cuts at: 4856 5352 4856 Size: 496 5157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5080 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2576 2685 3714 3799 4870 2576 Size: 109 1029 85 1071 3359 Fragments arranged by size: 3359 1071 1029 109 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2532 2576 2685 2908 2917 2972 Size: 399 454 288 44 109 223 9 55 Cuts at: 2972 3714 3799 4870 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 454 399 288 223 109 85 55 44 9 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3008 844 Size: 2164 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2772 4148 4322 65 Size: 1183 1524 1376 174 1396 Fragments arranged by size: 1524 1396 1376 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4297 4484 1700 Size: 110 2487 187 2869 Fragments arranged by size: 2869 2487 187 110 BsrGI T'GTAC_A Cuts at: 96 2838 2896 96 Size: 2742 58 2853 Fragments arranged by size: 2853 2742 58 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 2407 2569 3713 4725 2407 Size: 162 1144 1012 3335 Fragments arranged by size: 3335 1144 1012 162 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5653 BstXI CCAn_nnnn'nTGG Cuts at: 2125 2251 2263 2287 2125 Size: 126 12 24 5491 Fragments arranged by size: 5491 126 24 12 BstYI r'GATC_y Cuts at: 1972 2934 2983 3350 3735 3752 5204 5290 5302 Size: 962 49 367 385 17 1452 86 12 Cuts at: 5302 5648 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 962 385 367 346 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2358 513 Size: 552 939 354 3808 Fragments arranged by size: 3808 939 552 354 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2653 2907 487 Size: 683 56 1427 254 3233 Fragments arranged by size: 3233 1427 683 254 56 BtsI GCAGTG_nn' Cuts at: 2628 3169 3963 3983 2628 Size: 541 794 20 4298 Fragments arranged by size: 4298 794 541 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DraIII CAC_nnn'GTG Cuts at: 2527 2527 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2253 2355 Size: 54 1425 50 275 18 285 138 102 Cuts at: 2355 2379 2826 3100 3989 5618 8 Size: 24 447 274 889 1629 43 Fragments arranged by size: 1629 1425 889 447 285 275 274 138 102 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2762 3583 1353 Size: 1409 821 3423 Fragments arranged by size: 3423 1409 821 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2010 2669 3716 4313 5100 5368 1239 Size: 393 378 659 1047 597 787 268 1524 Fragments arranged by size: 1524 1047 787 659 597 393 378 268 EcoNI CCTnn'n_nnAGG Cuts at: 2704 2704 Size: 5653 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FspI TGC'GCA Cuts at: 2431 4159 2431 Size: 1728 3925 HaeII r_GCGC'y Cuts at: 1678 2619 4800 1678 Size: 941 2181 2531 Fragments arranged by size: 2531 2181 941 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2050 2082 4294 4326 4368 Size: 32 135 32 478 32 2212 32 42 Cuts at: 4368 4400 1373 Size: 32 2626 Fragments arranged by size: 2626 2212 478 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3243 669 Size: 988 281 1059 246 3079 Fragments arranged by size: 3079 1059 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KasI G'GCGC_C Cuts at: 2615 2615 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4767 4951 1305 Size: 3462 184 2007 Fragments arranged by size: 3462 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 2255 2381 2828 10 Size: 54 1425 343 285 138 126 447 2835 Fragments arranged by size: 2835 1425 447 343 285 138 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 2411 2834 2855 3611 3970 4129 518 Size: 648 1245 423 21 756 359 159 2042 Fragments arranged by size: 2042 1245 756 648 423 359 159 21 MspA1I CmG'CkG Cuts at: 1606 2080 2596 2675 2689 3069 3746 4894 5139 Size: 474 516 79 14 380 677 1148 245 Cuts at: 5139 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 516 474 380 245 79 14 NarI GG'CG_CC Cuts at: 2616 2616 Size: 5653 NcoI C'CATG_G Cuts at: 513 1065 2004 2358 513 Size: 552 939 354 3808 Fragments arranged by size: 3808 939 552 354 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NruI TCG'CGA Cuts at: 2892 2892 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 2326 1348 Size: 978 4675 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PfoI T'CCnGG_A Cuts at: 2068 2068 Size: 5653 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PstI C_TGCA'G Cuts at: 830 3005 830 Size: 2175 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2596 2675 2689 2596 Size: 79 14 5560 Fragments arranged by size: 5560 79 14 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2844 2875 3001 4136 4817 5008 826 Size: 219 1799 31 126 1135 681 191 1471 Fragments arranged by size: 1799 1471 1135 681 219 191 126 31 SfoI GGC'GCC Cuts at: 2617 2617 Size: 5653 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2420 2980 1872 Size: 548 560 4545 Fragments arranged by size: 4545 560 548 SmlI C'TyrA_G Cuts at: 820 1017 1943 2560 3757 4658 4920 820 Size: 197 926 617 1197 901 262 1553 Fragments arranged by size: 1553 1197 926 901 617 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2004 2358 3092 513 Size: 552 939 354 734 3074 Fragments arranged by size: 3074 939 734 552 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2194 2597 3680 3697 3850 4035 4480 Size: 1255 614 403 1083 17 153 185 445 Cuts at: 4480 325 Size: 1498 Fragments arranged by size: 1498 1255 1083 614 445 403 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2310 2838 Size: 274 80 33 51 1443 207 126 528 Cuts at: 2838 2896 3899 5611 96 Size: 58 1003 1712 138 Fragments arranged by size: 1712 1443 1003 528 274 207 138 126 80 58 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2127 2159 2456 2488 5613 5645 2127 Size: 32 297 32 3125 32 2135 Fragments arranged by size: 3125 2135 297 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2418 2978 1870 Size: 548 560 4545 Fragments arranged by size: 4545 560 548 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI