Restriction map for
pFN21AE0891
(Linear) (Six-base) MAPSORT of: KIEE0891.seq Check: 8535 from: 1 to: 948
KIEE0891.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 0 776 948
Size: 776 172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 948
Size: 5 943
AflII C'TTAA_G
Cuts at: 0 555 948
Size: 555 393
AhdI GACnn_n'nnGTC
Cuts at: 0 59 948
Size: 59 889
AlwNI CAG_nnn'CTG
Cuts at: 0 697 878 948
Size: 697 181 70
Fragments arranged by size:
697 181 70
AvaI C'yCGr_G
Cuts at: 0 413 948
Size: 413 535
BanI G'GyrC_C
Cuts at: 0 383 610 948
Size: 383 227 338
Fragments arranged by size:
383 338 227
BanII G_rGCy'C
Cuts at: 0 912 948
Size: 912 36
BbeI G_GCGC'C
Cuts at: 0 614 948
Size: 614 334
BbsI GAAGACnn'nnnn_
Cuts at: 0 11 334 948
Size: 11 323 614
Fragments arranged by size:
614 323 11
BbvCI CC'TCA_GC
Cuts at: 0 690 948
Size: 690 258
BglI GCCn_nnn'nGGC
Cuts at: 0 151 948
Size: 151 797
Bme1580I G_kGCm'C
Cuts at: 0 239 527 903 948
Size: 239 288 376 45
Fragments arranged by size:
376 288 239 45
BmgBI CAC'GTC
Cuts at: 0 462 948
Size: 462 486
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 48 931 948
Size: 48 883 17
Fragments arranged by size:
883 48 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 948
Size: 664 284
Bpu10I CC'TnA_GC
Cuts at: 0 690 948
Size: 690 258
BsaAI yAC'GTr
Cuts at: 0 372 948
Size: 372 576
BsaHI Gr'CG_yC
Cuts at: 0 611 948
Size: 611 337
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 451 481 948
Size: 451 30 467
Fragments arranged by size:
467 451 30
BsiHKAI G_wGCw'C
Cuts at: 0 571 680 948
Size: 571 109 268
Fragments arranged by size:
571 268 109
Bsp1286I G_dGCh'C
Cuts at: 0 239 527 571 680 903 912 948
Size: 239 288 44 109 223 9 36
Fragments arranged by size:
288 239 223 109 44 36 9
BsrDI GCAATG_nn'
Cuts at: 0 767 948
Size: 767 181
BsrGI T'GTAC_A
Cuts at: 0 833 891 948
Size: 833 58 57
Fragments arranged by size:
833 58 57
BssSI C'ACGA_G
Cuts at: 0 402 564 948
Size: 402 162 384
Fragments arranged by size:
402 384 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 120 246 258 282 948
Size: 120 126 12 24 666
Fragments arranged by size:
666 126 120 24 12
BstYI r'GATC_y
Cuts at: 0 929 948
Size: 929 19
BtgI C'CryG_G
Cuts at: 0 353 948
Size: 353 595
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 648 902 948
Size: 648 254 46
Fragments arranged by size:
648 254 46
BtsI GCAGTG_nn'
Cuts at: 0 623 948
Size: 623 325
DraIII CAC_nnn'GTG
Cuts at: 0 522 948
Size: 522 426
EaeI y'GGCC_r
Cuts at: 0 110 248 350 374 821 948
Size: 110 138 102 24 447 127
Fragments arranged by size:
447 138 127 110 102 24
EarI CTCTTCn'nnn_
Cuts at: 0 757 948
Size: 757 191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 664 948
Size: 5 659 284
Fragments arranged by size:
659 284 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 699 948
Size: 699 249
FspI TGC'GCA
Cuts at: 0 426 948
Size: 426 522
HaeII r_GCGC'y
Cuts at: 0 614 948
Size: 614 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 45 77 948
Size: 45 32 871
Fragments arranged by size:
871 45 32
KasI G'GCGC_C
Cuts at: 0 610 948
Size: 610 338
MscI TGG'CCA
Cuts at: 0 112 250 376 823 948
Size: 112 138 126 447 125
Fragments arranged by size:
447 138 126 125 112
MslI CAynn'nnrTG
Cuts at: 0 406 829 850 948
Size: 406 423 21 98
Fragments arranged by size:
423 406 98 21
MspA1I CmG'CkG
Cuts at: 0 75 591 670 684 948
Size: 75 516 79 14 264
Fragments arranged by size:
516 264 79 75 14
NarI GG'CG_CC
Cuts at: 0 611 948
Size: 611 337
NcoI C'CATG_G
Cuts at: 0 353 948
Size: 353 595
NruI TCG'CGA
Cuts at: 0 887 948
Size: 887 61
PasI CC'CwG_GG
Cuts at: 0 321 948
Size: 321 627
PfoI T'CCnGG_A
Cuts at: 0 63 948
Size: 63 885
PvuII CAG'CTG
Cuts at: 0 591 670 684 948
Size: 591 79 14 264
Fragments arranged by size:
591 264 79 14
SfcI C'TryA_G
Cuts at: 0 839 870 948
Size: 839 31 78
Fragments arranged by size:
839 78 31
SfoI GGC'GCC
Cuts at: 0 612 948
Size: 612 336
SmaI CCC'GGG
Cuts at: 0 415 948
Size: 415 533
SmlI C'TyrA_G
Cuts at: 0 555 948
Size: 555 393
StyI C'CwwG_G
Cuts at: 0 353 948
Size: 353 595
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 189 592 948
Size: 189 403 356
Fragments arranged by size:
403 356 189
TatI w'GTAC_w
Cuts at: 0 179 305 833 891 948
Size: 179 126 528 58 57
Fragments arranged by size:
528 179 126 58 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 122 154 451 483 948
Size: 122 32 297 32 465
Fragments arranged by size:
465 297 122 32 32
XmaI C'CCGG_G
Cuts at: 0 413 948
Size: 413 535
Enzymes that do cut:
AccI AcuI AflII AhdI AlwNI AvaI BanI BanII
BbeI BbsI BbvCI BglI Bme1580I BmgBI BplI BpmI
Bpu10I BsaAI BsaHI BsaXI BsiHKAI Bsp1286I BsrDI BsrGI
BssSI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI
EarI Eco57MI EcoNI FspI HaeII Hin4I KasI MscI
MslI MspA1I NarI NcoI NruI PasI PfoI PvuII
SfcI SfoI SmaI SmlI StyI TaqII TatI TstI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BpuEI BsaI BsaBI BsaWI BseRI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoO109I EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NotI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmnI ZraI