Restriction map for
pFN21AE0890
(Linear) (Six-base) MAPSORT of: KIEE0890.seq Check: 5060 from: 1 to: 939
KIEE0890.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 613 939
Size: 613 326
BamHI G'GATC_C
Cuts at: 0 446 939
Size: 446 493
BanII G_rGCy'C
Cuts at: 0 900 939
Size: 900 39
BbvCI CC'TCA_GC
Cuts at: 0 187 939
Size: 187 752
BclI T'GATC_A
Cuts at: 0 107 839 939
Size: 107 732 100
Fragments arranged by size:
732 107 100
BfrBI ATG'CAT
Cuts at: 0 638 939
Size: 638 301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 922 939
Size: 783 139 17
Fragments arranged by size:
783 139 17
Bpu10I CC'TnA_GC
Cuts at: 0 187 939
Size: 187 752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 661 939
Size: 538 123 278
Fragments arranged by size:
538 278 123
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 472 939
Size: 28 444 467
Fragments arranged by size:
467 444 28
BseYI C'CCAG_C
Cuts at: 0 117 491 939
Size: 117 374 448
Fragments arranged by size:
448 374 117
BsiEI CG_ry'CG
Cuts at: 0 363 939
Size: 363 576
Bsp1286I G_dGCh'C
Cuts at: 0 900 939
Size: 900 39
BspHI T'CATG_A
Cuts at: 0 404 939
Size: 404 535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 939
Size: 134 805
BsrDI GCAATG_nn'
Cuts at: 0 847 939
Size: 847 92
BsrGI T'GTAC_A
Cuts at: 0 830 939
Size: 830 109
BstEII G'GTnAC_C
Cuts at: 0 666 939
Size: 666 273
BstYI r'GATC_y
Cuts at: 0 446 939
Size: 446 493
Bsu36I CC'TnA_GG
Cuts at: 0 874 939
Size: 874 65
BtsI GCAGTG_nn'
Cuts at: 0 27 939
Size: 27 912
EaeI y'GGCC_r
Cuts at: 0 58 371 654 939
Size: 58 313 283 285
Fragments arranged by size:
313 285 283 58
EarI CTCTTCn'nnn_
Cuts at: 0 916 939
Size: 916 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 922 939
Size: 783 139 17
Fragments arranged by size:
783 139 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 717 939
Size: 717 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 599 631 939
Size: 29 570 32 308
Fragments arranged by size:
570 308 32 29
HindIII A'AGCT_T
Cuts at: 0 707 939
Size: 707 232
MscI TGG'CCA
Cuts at: 0 60 373 656 939
Size: 60 313 283 283
Fragments arranged by size:
313 283 283 60
MslI CAynn'nnrTG
Cuts at: 0 172 349 403 633 939
Size: 172 177 54 230 306
Fragments arranged by size:
306 230 177 172 54
MspA1I CmG'CkG
Cuts at: 0 495 939
Size: 495 444
NsiI A_TGCA'T
Cuts at: 0 640 939
Size: 640 299
NspI r_CATG'y
Cuts at: 0 102 255 939
Size: 102 153 684
Fragments arranged by size:
684 153 102
PfoI T'CCnGG_A
Cuts at: 0 41 442 939
Size: 41 401 497
Fragments arranged by size:
497 401 41
PvuI CG_AT'CG
Cuts at: 0 363 939
Size: 363 576
PvuII CAG'CTG
Cuts at: 0 495 939
Size: 495 444
SmlI C'TyrA_G
Cuts at: 0 553 676 939
Size: 553 123 263
Fragments arranged by size:
553 263 123
SpeI A'CTAG_T
Cuts at: 0 429 939
Size: 429 510
SphI G_CATG'C
Cuts at: 0 255 939
Size: 255 684
TatI w'GTAC_w
Cuts at: 0 830 939
Size: 830 109
XbaI T'CTAG_A
Cuts at: 0 327 939
Size: 327 612
XmnI GAAnn'nnTTC
Cuts at: 0 859 939
Size: 859 80
Enzymes that do cut:
AhdI BamHI BanII BbvCI BclI BfrBI BpmI Bpu10I
BpuEI BseRI BseYI BsiEI Bsp1286I BspHI BspMI BsrDI
BsrGI BstEII BstYI Bsu36I BtsI EaeI EarI Eco57MI
FalI Hin4I HindIII MscI MslI MspA1I NsiI NspI
PfoI PvuI PvuII SmlI SpeI SphI TatI XbaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BanI
BbeI BbsI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XcmI
XhoI XmaI ZraI