Restriction map for
pFN21AE0880
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRV FauI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MflI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Asp700I 900 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 697 GTGCTC G[AT]GC[AT]^C
AvrII 600 CCTAGG C^CTAGG
BclI 812 TGATCA T^GATCA
BlnI 600 CCTAGG C^CTAGG
BsaAI 768 CACGTA [CT]AC^GT[GA]
BseRI 921 CTCCTC GAGGAG(10/8)
BsiHKAI 697 GTGCTC G[AT]GC[AT]^C
BsmFI 878 GGGAC GGGAC(10/14)
Bsp1407I 824 TGTACA T^GTACA
BspHI 308 TCATGA T^CATGA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrDI 349 GCAATG GCAATG(2/0)
BsrGI 824 TGTACA T^GTACA
BstEII 225 GGTAACC G^GT[ACGT]ACC
BstPI 225 GGTAACC G^GT[ACGT]ACC
DsaI 412 CCACGG C^C[GA][CT]GG
EcoO65I 225 GGTAACC G^GT[ACGT]ACC
EcoRI 31 GAATTC G^AATTC
EcoT22I 633 ATGCAT ATGCA^T
FbaI 812 TGATCA T^GATCA
HgaI 805 GACGC GACGC(5/10)
HincII 205 GTTGAC GT[CT]^[GA]AC
HindII 205 GTTGAC GT[CT]^[GA]AC
HphI 447 GGTGA GGTGA(8/7)
MaeII 769 ACGT A^CGT
MseI 847 TTAA T^TAA
MwoI 346 GCTGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 356 CATATG CA^TATG
NsiI 633 ATGCAT ATGCA^T
RcaI 308 TCATGA T^CATGA
SfcI 861 CTACAG C^T[GA][CT]AG
SmlI 700 CTCAAG C^T[CT][GA]AG
SspBI 824 TGTACA T^GTACA
StuI 704 AGGCCT AGG^CCT
TfiI 917 GAATC G^A[AT]TC
Tru9I 847 TTAA T^TAA
XmnI 900 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 31 GAATTC [GA]^AATT[CT]
849 AAATTC
ApoI 31 GAATTC [GA]^AATT[CT]
849 AAATTC
BbsI 658 GTCTTC GAAGAC(2/6)
670 GTCTTC
BmyI 697 GTGCTC G[AGT]GC[ACT]^C
765 GGGCAC
BpuAI 658 GTCTTC GAAGAC(2/6)
670 GTCTTC
BsmAI 643 GTCTC GTCTC(1/5)
739 GTCTC
Bsp1286I 697 GTGCTC G[AGT]GC[ACT]^C
765 GGGCAC
Cac8I 243 GCTGGC GC[ACGT]^[ACGT]GC
695 GCGTGC
DdeI 236 CTAAG C^T[ACGT]AG
868 CTGAG
DpnII 361 GATC ^GATC
813 GATC
EarI 88 CTCTTC CTCTTC(1/4)
904 CTCTTC
Ksp632I 88 CTCTTC CTCTTC(1/4)
904 CTCTTC
MboI 361 GATC ^GATC
813 GATC
NdeII 361 GATC ^GATC
813 GATC
NlaIV 678 GGTTCC GG[ACGT]^[ACGT]CC
721 GGTTCC
PflMI 252 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
513 CCAGGAAGTGG
PleI 408 GAGTC GAGTC(4/5)
656 GAGTC
Sau3AI 361 GATC ^GATC
813 GATC
TaqI 363 TCGA T^CGA
654 TCGA
Tsp45I 536 GTGAC ^GT[GC]AC
835 GTGAC
TthHB8I 363 TCGA T^CGA
654 TCGA
Van91I 252 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
513 CCAGGAAGTGG
XcmI 191 CCAACCTCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
248 CCAACCATCTCTTGG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI BalI BbvI
BfaI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboII MluNI
MnlI MscI MslI MvaI NlaIII
RsaI ScrFI SfaNI StyI TseI
Tsp509I TspRI