Restriction map for
pFN21AE0880
(Linear) (Six-base) MAPSORT of: KIEE0880.seq Check: 2200 from: 1 to: 927
KIEE0880.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 927
Size: 574 353
AleI CACnn'nnGTG
Cuts at: 0 832 927
Size: 832 95
ApoI r'AATT_y
Cuts at: 0 31 849 927
Size: 31 818 78
Fragments arranged by size:
818 78 31
AvrII C'CTAG_G
Cuts at: 0 600 927
Size: 600 327
BbsI GAAGACnn'nnnn_
Cuts at: 0 651 663 927
Size: 651 12 264
Fragments arranged by size:
651 264 12
BclI T'GATC_A
Cuts at: 0 812 927
Size: 812 115
BfrBI ATG'CAT
Cuts at: 0 635 927
Size: 635 292
Bme1580I G_kGCm'C
Cuts at: 0 769 927
Size: 769 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 927
Size: 685 242
BsaAI yAC'GTr
Cuts at: 0 770 927
Size: 770 157
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 912 927
Size: 912 15
BseYI C'CCAG_C
Cuts at: 0 436 927
Size: 436 491
BsiHKAI G_wGCw'C
Cuts at: 0 701 927
Size: 701 226
Bsp1286I G_dGCh'C
Cuts at: 0 701 769 927
Size: 701 68 158
Fragments arranged by size:
701 158 68
BspHI T'CATG_A
Cuts at: 0 308 927
Size: 308 619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 927
Size: 134 793
BsrDI GCAATG_nn'
Cuts at: 0 356 927
Size: 356 571
BsrGI T'GTAC_A
Cuts at: 0 824 927
Size: 824 103
BstEII G'GTnAC_C
Cuts at: 0 225 927
Size: 225 702
BtgI C'CryG_G
Cuts at: 0 412 927
Size: 412 515
BtsI GCAGTG_nn'
Cuts at: 0 815 839 927
Size: 815 24 88
Fragments arranged by size:
815 88 24
EaeI y'GGCC_r
Cuts at: 0 245 371 521 927
Size: 245 126 150 406
Fragments arranged by size:
406 245 150 126
EarI CTCTTCn'nnn_
Cuts at: 0 94 910 927
Size: 94 816 17
Fragments arranged by size:
816 94 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 766 927
Size: 766 161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 927
Size: 574 353
EcoRI G'AATT_C
Cuts at: 0 31 927
Size: 31 896
HincII GTy'rAC
Cuts at: 0 207 927
Size: 207 720
MscI TGG'CCA
Cuts at: 0 247 373 523 927
Size: 247 126 150 404
Fragments arranged by size:
404 247 150 126
MslI CAynn'nnrTG
Cuts at: 0 581 630 820 832 927
Size: 581 49 190 12 95
Fragments arranged by size:
581 190 95 49 12
NdeI CA'TA_TG
Cuts at: 0 357 927
Size: 357 570
NsiI A_TGCA'T
Cuts at: 0 637 927
Size: 637 290
PflMI CCAn_nnn'nTGG
Cuts at: 0 258 519 927
Size: 258 261 408
Fragments arranged by size:
408 261 258
SfcI C'TryA_G
Cuts at: 0 861 927
Size: 861 66
SmlI C'TyrA_G
Cuts at: 0 700 927
Size: 700 227
StuI AGG'CCT
Cuts at: 0 706 927
Size: 706 221
StyI C'CwwG_G
Cuts at: 0 200 317 600 689 927
Size: 200 117 283 89 238
Fragments arranged by size:
283 238 200 117 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 927
Size: 435 492
TatI w'GTAC_w
Cuts at: 0 824 927
Size: 824 103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 255 927
Size: 198 57 672
Fragments arranged by size:
672 198 57
XmnI GAAnn'nnTTC
Cuts at: 0 904 927
Size: 904 23
Enzymes that do cut:
AcuI AleI ApoI AvrII BbsI BclI BfrBI Bme1580I
BpuEI BsaAI BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BsrGI BstEII BtgI BtsI EaeI EarI EciI
Eco57MI EcoRI HincII MscI MslI NdeI NsiI PflMI
SfcI SmlI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BglI BglII BlpI BmgBI BmrI
BmtI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI