(Circular) (Six-base) MAPSORT of: pFN21AE0875.seq Check: 25 from: 1 to: 5746 pFN21AE0875 5746 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3089 1937 Size: 1152 4594 Acc65I G'GTAC_C Cuts at: 2852 3067 2852 Size: 215 5531 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3809 5193 5461 1632 Size: 2177 1384 268 1917 Fragments arranged by size: 2177 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 2690 1166 Size: 1524 4222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5746 AlwNI CAG_nnn'CTG Cuts at: 3489 5061 3489 Size: 1572 4174 ApaLI G'TGCA_C Cuts at: 3803 4959 3803 Size: 1156 4590 ApoI r'AATT_y Cuts at: 1493 2126 2557 2779 3055 3281 3433 5542 1493 Size: 633 431 222 276 226 152 2109 1697 Fragments arranged by size: 2109 1697 633 431 276 226 222 152 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 3071 1870 Size: 73 1128 4545 Fragments arranged by size: 4545 1128 73 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2852 3067 4522 618 Size: 325 217 627 1065 215 1455 1842 Fragments arranged by size: 1842 1455 1065 627 325 217 215 BanII G_rGCy'C Cuts at: 1391 3065 1391 Size: 1674 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2800 928 Size: 971 901 3874 Fragments arranged by size: 3874 971 901 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3937 3971 1903 Size: 34 2000 34 3678 Fragments arranged by size: 3678 2000 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2144 3638 4848 2144 Size: 1494 1210 3042 Fragments arranged by size: 3042 1494 1210 BclI T'GATC_A Cuts at: 1548 2838 1548 Size: 1290 4456 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4357 136 Size: 107 122 71 631 605 2685 1525 Fragments arranged by size: 2685 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2063 5741 2063 Size: 3678 2068 BlpI GC'TnA_GC Cuts at: 2818 3163 2818 Size: 345 5401 Bme1580I G_kGCm'C Cuts at: 1790 3807 4963 1790 Size: 2017 1156 2573 Fragments arranged by size: 2573 2017 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4435 442 Size: 416 53 83 399 24 2060 958 1753 Fragments arranged by size: 2060 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4406 1239 Size: 3167 2579 Bpu10I CC'TnA_GC Cuts at: 2501 2501 Size: 5746 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2633 2871 3007 3871 4736 5034 2633 Size: 238 136 864 865 298 3345 Fragments arranged by size: 3345 865 864 298 238 136 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4409 882 Size: 617 2910 2219 Fragments arranged by size: 2910 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2814 3537 493 Size: 711 1610 723 2702 Fragments arranged by size: 2702 1610 723 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3553 3935 4570 275 Size: 53 83 186 936 2020 382 635 1451 Fragments arranged by size: 2020 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2546 4179 4577 4851 4998 5409 2546 Size: 1633 398 274 147 411 2883 Fragments arranged by size: 2883 1633 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5521 Fragments arranged by size: 5521 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2311 1186 Size: 1125 4621 BseYI C'CCAG_C Cuts at: 4949 5445 4949 Size: 496 5250 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2406 2411 1163 Size: 573 670 5 4498 Fragments arranged by size: 4498 670 573 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2819 3807 3892 4963 2819 Size: 988 85 1071 3602 Fragments arranged by size: 3602 1071 988 85 BsmI GAATG_Cn' Cuts at: 3257 3350 3257 Size: 93 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2819 3065 3807 3892 4963 1391 Size: 399 1029 246 742 85 1071 2174 Fragments arranged by size: 2174 1071 1029 742 399 246 85 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3101 844 Size: 2257 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2224 2418 4241 4415 65 Size: 1183 976 194 1823 174 1396 Fragments arranged by size: 1823 1396 1183 976 194 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4390 4577 1700 Size: 110 2580 187 2869 Fragments arranged by size: 2869 2580 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5746 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5746 BstYI r'GATC_y Cuts at: 1972 2063 3076 3443 3828 3845 5297 5383 5395 Size: 91 1013 367 385 17 1452 86 12 Cuts at: 5395 5741 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1013 385 367 346 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2688 513 Size: 552 1623 3571 Fragments arranged by size: 3571 1623 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 2394 3262 4056 4076 2394 Size: 868 794 20 4064 Fragments arranged by size: 4064 868 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2119 4753 809 Size: 507 803 2634 1802 Fragments arranged by size: 2634 1802 803 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2466 3193 4082 Size: 54 1425 50 275 18 636 727 889 Cuts at: 4082 5711 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 727 636 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3676 1353 Size: 2323 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3809 4406 5193 5461 1239 Size: 393 2177 597 787 268 1524 Fragments arranged by size: 2177 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2504 2504 Size: 5746 EcoRI G'AATT_C Cuts at: 2779 3055 3433 2779 Size: 276 378 5092 Fragments arranged by size: 5092 378 276 EcoRV GAT'ATC Cuts at: 2305 2697 2305 Size: 392 5354 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 4893 1678 Size: 3215 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2478 2510 4387 4419 4461 Size: 32 135 32 906 32 1877 32 42 Cuts at: 4461 4493 1373 Size: 32 2626 Fragments arranged by size: 2626 1877 906 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2421 3090 3336 669 Size: 988 281 483 669 246 3079 Fragments arranged by size: 3079 988 669 483 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 2856 3071 2856 Size: 215 5531 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4860 5044 1305 Size: 3555 184 2007 Fragments arranged by size: 3555 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2468 10 Size: 54 1425 343 636 3288 Fragments arranged by size: 3288 1425 636 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 2690 3704 4063 4222 518 Size: 648 1101 423 1014 359 159 2042 Fragments arranged by size: 2042 1101 1014 648 423 359 159 MspA1I CmG'CkG Cuts at: 1606 2564 3162 3839 4987 5232 1606 Size: 958 598 677 1148 245 2120 Fragments arranged by size: 2120 1148 958 677 598 245 NcoI C'CATG_G Cuts at: 513 1065 2688 513 Size: 552 1623 3571 Fragments arranged by size: 3571 1623 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 2631 3104 4649 1132 Size: 97 1402 473 1545 2229 Fragments arranged by size: 2229 1545 1402 473 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5746 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5746 PfoI T'CCnGG_A Cuts at: 2298 2298 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PmlI CAC'GTG Cuts at: 2814 2814 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PstI C_TGCA'G Cuts at: 830 3098 830 Size: 2268 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2564 2564 Size: 5746 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SfcI C'TryA_G Cuts at: 826 1045 2980 3094 4229 4910 5101 826 Size: 219 1935 114 1135 681 191 1471 Fragments arranged by size: 1935 1471 1135 681 219 191 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 3073 1872 Size: 1201 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2648 2886 2986 3850 4751 5013 Size: 197 926 705 238 100 864 901 262 Cuts at: 5013 820 Size: 1553 Fragments arranged by size: 1553 926 901 864 705 262 238 197 100 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 3104 1132 Size: 1972 3774 SspI AAT'ATT Cuts at: 5 52 2611 2637 2680 3020 3670 5 Size: 47 2559 26 43 340 650 2081 Fragments arranged by size: 2559 2081 650 340 47 43 26 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 2688 3185 513 Size: 552 1623 497 3074 Fragments arranged by size: 3074 1623 552 497 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2087 2974 3773 3790 3943 4128 4573 Size: 1255 507 887 799 17 153 185 445 Cuts at: 4573 325 Size: 1498 Fragments arranged by size: 1498 1255 887 799 507 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2024 2271 3992 Size: 274 80 33 51 1443 47 247 1721 Cuts at: 3992 5704 96 Size: 1712 138 Fragments arranged by size: 1721 1712 1443 274 247 138 80 51 47 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2373 2405 5706 5738 2373 Size: 32 3301 32 2381 Fragments arranged by size: 3301 2381 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5746 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 3071 1870 Size: 1201 4545 XmnI GAAnn'nnTTC Cuts at: 2130 2368 2557 3875 2130 Size: 238 189 1318 4001 Fragments arranged by size: 4001 1318 238 189 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI