(Circular) (Six-base) MAPSORT of: pFN21AE0874.seq Check: 3268 from: 1 to: 5659 pFN21AE0874 5659 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3002 1937 Size: 1065 4594 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2066 3722 5106 5374 1632 Size: 434 1656 1384 268 1917 Fragments arranged by size: 1917 1656 1384 434 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 2658 4558 5535 1203 Size: 1455 1900 977 1327 Fragments arranged by size: 1900 1455 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 2249 1166 Size: 1083 4576 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5659 AlwNI CAG_nnn'CTG Cuts at: 3402 4974 3402 Size: 1572 4087 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2036 2544 2968 3194 3346 5455 1493 Size: 543 508 424 226 152 2109 1697 Fragments arranged by size: 2109 1697 543 508 424 226 152 AseI AT'TA_AT Cuts at: 160 1032 2642 4213 160 Size: 872 1610 1571 1606 Fragments arranged by size: 1610 1606 1571 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2984 1870 Size: 73 1041 4545 Fragments arranged by size: 4545 1041 73 BamHI G'GATC_C Cuts at: 2989 3356 2989 Size: 367 5292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2980 4435 618 Size: 325 217 627 1193 1455 1842 Fragments arranged by size: 1842 1455 1193 627 325 217 BanII G_rGCy'C Cuts at: 1391 2978 1391 Size: 1587 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4688 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 1548 Size: 5659 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4270 136 Size: 107 122 71 631 605 2598 1525 Fragments arranged by size: 2598 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 3720 4876 1790 Size: 1930 1156 2573 Fragments arranged by size: 2573 1930 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3390 4348 442 Size: 416 53 83 399 24 1973 958 1753 Fragments arranged by size: 1973 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4319 1239 Size: 3080 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2093 3784 4649 4947 2093 Size: 1691 865 298 2805 Fragments arranged by size: 2805 1691 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4322 882 Size: 617 2823 2219 Fragments arranged by size: 2823 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3450 493 Size: 711 2246 2702 Fragments arranged by size: 2702 2246 711 BsaBI GATnn'nnATC Cuts at: 5653 5653 Size: 5659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3466 3848 4483 275 Size: 53 83 186 936 1933 382 635 1451 Fragments arranged by size: 1933 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5434 Fragments arranged by size: 5434 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5659 BseYI C'CCAG_C Cuts at: 2441 4862 5358 2441 Size: 2421 496 2742 Fragments arranged by size: 2742 2421 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2316 2321 1163 Size: 573 580 5 4501 Fragments arranged by size: 4501 580 573 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2094 3720 3805 4876 2094 Size: 1626 85 1071 2877 Fragments arranged by size: 2877 1626 1071 85 BsmI GAATG_Cn' Cuts at: 3170 3263 3170 Size: 93 5566 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2978 3720 3805 4876 1391 Size: 399 304 884 742 85 1071 2174 Fragments arranged by size: 2174 1071 884 742 399 304 85 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 2409 2556 3548 5278 2409 Size: 147 992 1730 2790 Fragments arranged by size: 2790 1730 992 147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3014 844 Size: 2170 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4154 4328 65 Size: 1183 2906 174 1396 Fragments arranged by size: 2906 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4303 4490 1700 Size: 110 2493 187 2869 Fragments arranged by size: 2869 2493 187 110 BsrGI T'GTAC_A Cuts at: 96 2778 96 Size: 2682 2977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 2060 1184 Size: 876 4783 BstXI CCAn_nnnn'nTGG Cuts at: 2032 2032 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654 Size: 1017 367 385 17 1452 86 12 346 Cuts at: 5654 1972 Size: 1977 Fragments arranged by size: 1977 1452 1017 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2322 2433 513 Size: 552 939 318 111 3739 Fragments arranged by size: 3739 939 552 318 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4920 Fragments arranged by size: 4920 683 56 BtsI GCAGTG_nn' Cuts at: 2304 3175 3969 3989 2304 Size: 871 794 20 3974 Fragments arranged by size: 3974 871 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2058 4666 809 Size: 507 742 2608 1802 Fragments arranged by size: 2608 1802 742 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 2607 3106 Size: 54 1425 50 275 18 489 288 499 Cuts at: 3106 3995 5624 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 499 489 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2698 2777 3589 1353 Size: 1345 79 812 3423 Fragments arranged by size: 3423 1345 812 79 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 3722 4319 5106 5374 1239 Size: 393 434 1656 597 787 268 1524 Fragments arranged by size: 1656 1524 787 597 434 393 268 EcoO109I rG'GnC_Cy Cuts at: 2445 2445 Size: 5659 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 EcoRV GAT'ATC Cuts at: 2399 2399 Size: 5659 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2730 2822 2730 Size: 92 5567 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 4806 1678 Size: 3128 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2388 2420 4300 4332 4374 Size: 32 135 32 816 32 1880 32 42 Cuts at: 4374 4406 1373 Size: 32 2626 Fragments arranged by size: 2626 1880 816 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 2712 748 Size: 1964 3695 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 2498 3258 2498 Size: 760 4899 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4773 4957 1305 Size: 3468 184 2007 Fragments arranged by size: 3468 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 2609 10 Size: 54 1425 343 489 288 3060 Fragments arranged by size: 3060 1425 489 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2030 2177 2249 2408 2603 2780 3617 Size: 648 864 147 72 159 195 177 837 Cuts at: 3617 3976 4135 518 Size: 359 159 2042 Fragments arranged by size: 2042 864 837 648 359 195 177 159 159 147 72 MspA1I CmG'CkG Cuts at: 1606 2441 3075 3752 4900 5145 1606 Size: 835 634 677 1148 245 2120 Fragments arranged by size: 2120 1148 835 677 634 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2322 2433 513 Size: 552 939 318 111 3739 Fragments arranged by size: 3739 939 552 318 111 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 3017 4562 1132 Size: 97 1788 1545 2229 Fragments arranged by size: 2229 1788 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4757 PfoI T'CCnGG_A Cuts at: 2447 2447 Size: 5659 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2445 2445 Size: 5659 PshAI GACnn'nnGTC Cuts at: 1613 2744 1613 Size: 1131 4528 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PstI C_TGCA'G Cuts at: 830 2476 2513 3011 830 Size: 1646 37 498 3478 Fragments arranged by size: 3478 1646 498 37 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 PvuII CAG'CTG Cuts at: 2441 2441 Size: 5659 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SapI GCTCTTCn'nnn_ Cuts at: 2698 2698 Size: 5659 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 2472 2509 2890 3007 4142 4823 5014 Size: 219 1427 37 381 117 1135 681 191 Cuts at: 5014 826 Size: 1471 Fragments arranged by size: 1471 1427 1135 681 381 219 191 117 37 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2072 3763 4664 4926 820 Size: 197 926 129 1691 901 262 1553 Fragments arranged by size: 1691 1553 926 901 262 197 129 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 2754 3583 5 Size: 47 2702 829 2081 Fragments arranged by size: 2702 2081 829 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5659 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 2433 3098 513 Size: 552 939 318 111 665 3074 Fragments arranged by size: 3074 939 665 552 318 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2079 3686 3703 3856 4041 4486 325 Size: 1255 499 1607 17 153 185 445 1498 Fragments arranged by size: 1607 1498 1255 499 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2476 2778 Size: 274 80 33 51 1443 204 295 302 Cuts at: 2778 3905 5617 96 Size: 1127 1712 138 Fragments arranged by size: 1712 1443 1127 302 295 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2221 2253 5619 5651 2221 Size: 32 3366 32 2229 Fragments arranged by size: 3366 2229 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 2040 3788 2040 Size: 1748 3911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI