(Circular) (Six-base) MAPSORT of: pFN21AE0873.seq Check: 436 from: 1 to: 5671 pFN21AE0873 5671 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3481 278 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 1937 Size: 1077 4594 Acc65I G'GTAC_C Cuts at: 2992 2992 Size: 5671 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3734 5118 5386 1632 Size: 2102 1384 268 1917 Fragments arranged by size: 2102 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 2184 2223 2723 4570 5547 1203 Size: 981 39 500 1847 977 1327 Fragments arranged by size: 1847 1327 981 977 500 39 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 4400 4400 Size: 5671 AleI CACnn'nnGTG Cuts at: 1166 2147 2234 2591 1166 Size: 981 87 357 4246 Fragments arranged by size: 4246 981 357 87 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5671 AlwNI CAG_nnn'CTG Cuts at: 2080 2161 3414 4986 2080 Size: 81 1253 1572 2765 Fragments arranged by size: 2765 1572 1253 81 ApaI G_GGCC'C Cuts at: 2517 2655 2517 Size: 138 5533 ApaLI G'TGCA_C Cuts at: 2452 3728 4884 2452 Size: 1276 1156 3239 Fragments arranged by size: 3239 1276 1156 ApoI r'AATT_y Cuts at: 1493 2980 3206 3358 5467 1493 Size: 1487 226 152 2109 1697 Fragments arranged by size: 2109 1697 1487 226 152 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2996 1870 Size: 73 1053 4545 Fragments arranged by size: 4545 1053 73 BamHI G'GATC_C Cuts at: 3001 3368 3001 Size: 367 5304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2992 4447 618 Size: 325 217 627 1205 1455 1842 Fragments arranged by size: 1842 1455 1205 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2424 2517 2655 2990 1391 Size: 622 411 93 138 335 4072 Fragments arranged by size: 4072 622 411 335 138 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2824 928 Size: 971 925 3775 Fragments arranged by size: 3775 971 925 BbvCI CC'TCA_GC Cuts at: 2324 2324 Size: 5671 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3862 3896 1903 Size: 34 1925 34 3678 Fragments arranged by size: 3678 1925 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3563 4773 3563 Size: 1210 4461 BclI T'GATC_A Cuts at: 1548 1548 Size: 5671 BfrBI ATG'CAT Cuts at: 2413 2413 Size: 5671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2297 2357 4282 Size: 107 122 71 631 605 625 60 1925 Cuts at: 4282 136 Size: 1525 Fragments arranged by size: 1925 1525 631 625 605 122 107 71 60 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 3088 3088 Size: 5671 Bme1580I G_kGCm'C Cuts at: 1790 2241 2456 2517 2655 3732 4888 1790 Size: 451 215 61 138 1077 1156 2573 Fragments arranged by size: 2573 1156 1077 451 215 138 61 BmgBI CAC'GTC Cuts at: 1318 2226 1318 Size: 908 4763 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2657 2784 2858 Size: 416 53 83 399 24 1240 127 74 Cuts at: 2858 3402 4360 442 Size: 544 958 1753 Fragments arranged by size: 1753 1240 958 544 416 399 127 83 74 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 2231 4331 1239 Size: 938 54 2100 2579 Fragments arranged by size: 2579 2100 938 54 Bpu10I CC'TnA_GC Cuts at: 2324 2324 Size: 5671 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2899 3796 4661 4959 2899 Size: 897 865 298 3611 Fragments arranged by size: 3611 897 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2449 4334 882 Size: 617 950 1885 2219 Fragments arranged by size: 2219 1885 950 617 BsaAI yAC'GTr Cuts at: 493 1204 3462 493 Size: 711 2258 2702 Fragments arranged by size: 2702 2258 711 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3478 3860 4495 275 Size: 53 83 186 936 1945 382 635 1451 Fragments arranged by size: 1945 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4104 4502 4776 4923 5334 4104 Size: 398 274 147 411 4441 Fragments arranged by size: 4441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 2935 2965 1178 Size: 30 165 30 801 30 701 30 3884 Fragments arranged by size: 3884 801 701 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5671 BseYI C'CCAG_C Cuts at: 4874 5370 4874 Size: 496 5175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 1163 Size: 573 417 4681 Fragments arranged by size: 4681 573 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3121 3882 4910 1540 Size: 275 186 1120 761 1028 2301 Fragments arranged by size: 2301 1120 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2456 3732 3817 4888 2456 Size: 1276 85 1071 3239 Fragments arranged by size: 3239 1276 1071 85 BsmI GAATG_Cn' Cuts at: 3182 3275 3182 Size: 93 5578 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2241 2424 2456 2517 2655 2990 Size: 399 223 228 183 32 61 138 335 Cuts at: 2990 3732 3817 4888 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 335 228 223 183 138 85 61 32 BspEI T'CCGG_A Cuts at: 5334 5334 Size: 5671 BspHI T'CATG_A Cuts at: 2916 3560 5290 2916 Size: 644 1730 3297 Fragments arranged by size: 3297 1730 644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2265 3026 844 Size: 1421 761 3489 Fragments arranged by size: 3489 1421 761 BsrBI CCG'CTC Cuts at: 1394 1823 3558 1394 Size: 429 1735 3507 Fragments arranged by size: 3507 1735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4166 4340 65 Size: 1183 2918 174 1396 Fragments arranged by size: 2918 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4315 4502 1700 Size: 110 2505 187 2869 Fragments arranged by size: 2869 2505 187 110 BsrGI T'GTAC_A Cuts at: 96 2181 96 Size: 2085 3586 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5671 BssSI C'ACGA_G Cuts at: 3731 4743 3731 Size: 1012 4659 BstAPI GCAn_nnn'nTGC Cuts at: 794 2080 794 Size: 1286 4385 BstBI TT'CG_AA Cuts at: 4475 4475 Size: 5671 BstEII G'GTnAC_C Cuts at: 1184 2593 1184 Size: 1409 4262 BstXI CCAn_nnnn'nTGG Cuts at: 2437 2437 Size: 5671 BstYI r'GATC_y Cuts at: 1972 3001 3368 3753 3770 5222 5308 5320 5666 Size: 1029 367 385 17 1452 86 12 346 Cuts at: 5666 1972 Size: 1977 Fragments arranged by size: 1977 1452 1029 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2145 513 Size: 552 939 141 4039 Fragments arranged by size: 4039 939 552 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4932 Fragments arranged by size: 4932 683 56 BtsI GCAGTG_nn' Cuts at: 2852 3187 3981 4001 2852 Size: 335 794 20 4522 Fragments arranged by size: 4522 794 335 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5636 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DraIII CAC_nnn'GTG Cuts at: 2337 2337 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2376 2701 3118 Size: 54 1425 50 275 18 546 325 417 Cuts at: 3118 4007 5636 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 546 417 325 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 1537 Size: 275 1306 4090 Fragments arranged by size: 4090 1306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3601 1353 Size: 2248 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4258 4632 4778 387 Size: 1064 138 2669 374 146 1280 Fragments arranged by size: 2669 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2231 3734 4331 5118 5386 1239 Size: 393 545 54 1503 597 787 268 1524 Fragments arranged by size: 1524 1503 787 597 545 393 268 54 EcoO109I rG'GnC_Cy Cuts at: 2077 2158 2651 2652 2077 Size: 81 493 1 5096 Fragments arranged by size: 5096 493 81 1 EcoRI G'AATT_C Cuts at: 2980 3358 2980 Size: 378 5293 FspI TGC'GCA Cuts at: 4177 4177 Size: 5671 HaeII r_GCGC'y Cuts at: 1678 2331 4818 1678 Size: 653 2487 2531 Fragments arranged by size: 2531 2487 653 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2568 2600 4312 4344 4386 Size: 32 135 32 996 32 1712 32 42 Cuts at: 4386 4418 1373 Size: 32 2626 Fragments arranged by size: 2626 1712 996 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3015 3261 669 Size: 988 281 1077 246 3079 Fragments arranged by size: 3079 1077 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KpnI G_GTAC'C Cuts at: 2996 2996 Size: 5671 MfeI C'AATT_G Cuts at: 2613 3270 2613 Size: 657 5014 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2481 4785 4969 1305 Size: 1176 2304 184 2007 Fragments arranged by size: 2304 2007 1176 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 10 Size: 54 1425 343 546 3303 Fragments arranged by size: 3303 1425 546 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2147 2234 2591 2780 3629 3988 Size: 648 978 3 87 357 189 849 359 Cuts at: 3988 4147 518 Size: 159 2042 Fragments arranged by size: 2042 978 849 648 359 357 189 159 87 3 MspA1I CmG'CkG Cuts at: 1606 2044 3087 3764 4912 5157 1606 Size: 438 1043 677 1148 245 2120 Fragments arranged by size: 2120 1148 1043 677 438 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4180 Fragments arranged by size: 4180 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 3118 3118 Size: 5671 NsiI A_TGCA'T Cuts at: 2415 2415 Size: 5671 NspI r_CATG'y Cuts at: 1132 1229 2188 2727 3029 4574 1132 Size: 97 959 539 302 1545 2229 Fragments arranged by size: 2229 1545 959 539 302 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5671 PciI A'CATG_T Cuts at: 2184 2723 4570 2184 Size: 539 1847 3285 Fragments arranged by size: 3285 1847 539 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5671 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3740 3772 5253 5285 3740 Size: 32 1481 32 4126 Fragments arranged by size: 4126 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5671 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PspOMI G'GGCC_C Cuts at: 2513 2651 2513 Size: 138 5533 PstI C_TGCA'G Cuts at: 830 2387 3023 830 Size: 1557 636 3478 Fragments arranged by size: 3478 1557 636 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 PvuII CAG'CTG Cuts at: 2044 2044 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 3919 5631 3919 Size: 1712 3959 SexAI A'CCwGG_T Cuts at: 2429 2429 Size: 5671 SfcI C'TryA_G Cuts at: 826 1045 2383 2872 3019 4154 4835 5026 826 Size: 219 1338 489 147 1135 681 191 1471 Fragments arranged by size: 1471 1338 1135 681 489 219 191 147 SfiI GGCCn_nnn'nGGCC Cuts at: 2357 2357 Size: 5671 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2998 1872 Size: 1126 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2878 3775 4676 4938 820 Size: 197 926 935 897 901 262 1553 Fragments arranged by size: 1553 935 926 901 897 262 197 SnaBI TAC'GTA Cuts at: 493 3462 493 Size: 2969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 3029 1132 Size: 1897 3774 SspI AAT'ATT Cuts at: 5 52 3595 5 Size: 47 3543 2081 Fragments arranged by size: 3543 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5671 StyI C'CwwG_G Cuts at: 513 1065 2004 3110 513 Size: 552 939 1106 3074 Fragments arranged by size: 3074 1106 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3698 3715 3868 4053 4498 325 Size: 1255 2118 17 153 185 445 1498 Fragments arranged by size: 2118 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2945 3917 Size: 274 80 33 51 1443 204 764 972 Cuts at: 3917 5629 96 Size: 1712 138 Fragments arranged by size: 1712 1443 972 764 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5631 5663 5631 Size: 32 5639 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5671 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2437 2437 Size: 5671 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5671 XmaI C'CCGG_G Cuts at: 1870 2996 1870 Size: 1126 4545 XmnI GAAnn'nnTTC Cuts at: 2689 3800 2689 Size: 1111 4560 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3479 276 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI