(Circular) (Six-base) MAPSORT of: pFN21AE0868.seq Check: 5321 from: 1 to: 5620 pFN21AE0868 5620 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2705 2705 Size: 5620 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2963 1937 Size: 1026 4594 Acc65I G'GTAC_C Cuts at: 2941 2941 Size: 5620 AclI AA'CG_TT Cuts at: 1563 3747 4120 1563 Size: 2184 373 3063 Fragments arranged by size: 3063 2184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3683 5067 5335 1632 Size: 2051 1384 268 1917 Fragments arranged by size: 2051 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 4519 5496 1203 Size: 3316 977 1327 Fragments arranged by size: 3316 1327 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 2201 2717 1166 Size: 1035 516 4069 Fragments arranged by size: 4069 1035 516 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5620 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2891 2923 2000 Size: 32 859 32 4697 Fragments arranged by size: 4697 859 32 32 AlwNI CAG_nnn'CTG Cuts at: 2901 3363 4935 2901 Size: 462 1572 3586 Fragments arranged by size: 3586 1572 462 ApaLI G'TGCA_C Cuts at: 3677 4833 3677 Size: 1156 4464 ApoI r'AATT_y Cuts at: 1493 2009 2929 3155 3307 5416 1493 Size: 516 920 226 152 2109 1697 Fragments arranged by size: 2109 1697 920 516 226 152 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2945 1870 Size: 73 1002 4545 Fragments arranged by size: 4545 1002 73 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2209 2941 4396 618 Size: 325 217 627 422 732 1455 1842 Fragments arranged by size: 1842 1455 732 627 422 325 217 BanII G_rGCy'C Cuts at: 1391 2939 1391 Size: 1548 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2252 928 Size: 971 353 4296 Fragments arranged by size: 4296 971 353 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3811 3845 1903 Size: 34 1874 34 3678 Fragments arranged by size: 3678 1874 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 2088 1548 Size: 540 5080 BfrBI ATG'CAT Cuts at: 2533 2533 Size: 5620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4231 136 Size: 107 122 71 631 605 2559 1525 Fragments arranged by size: 2559 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5615 5615 Size: 5620 BlpI GC'TnA_GC Cuts at: 3037 3037 Size: 5620 Bme1580I G_kGCm'C Cuts at: 1790 2214 2762 3681 4837 1790 Size: 424 548 919 1156 2573 Fragments arranged by size: 2573 1156 919 548 424 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5620 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2106 2306 3351 Size: 416 53 83 399 24 689 200 1045 Cuts at: 3351 4309 442 Size: 958 1753 Fragments arranged by size: 1753 1045 958 689 416 399 200 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2752 4280 1239 Size: 1513 1528 2579 Fragments arranged by size: 2579 1528 1513 Bpu10I CC'TnA_GC Cuts at: 2490 2490 Size: 5620 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2639 3745 4610 4908 2639 Size: 1106 865 298 3351 Fragments arranged by size: 3351 1106 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4283 882 Size: 617 2784 2219 Fragments arranged by size: 2784 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3411 493 Size: 711 2207 2702 Fragments arranged by size: 2702 2207 711 BsaBI GATnn'nnATC Cuts at: 5614 5614 Size: 5620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 3809 4444 275 Size: 53 83 186 936 1894 382 635 1451 Fragments arranged by size: 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4053 4451 4725 4872 5283 4053 Size: 398 274 147 411 4390 Fragments arranged by size: 4390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5395 Fragments arranged by size: 5395 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2369 1186 Size: 1183 4437 BseYI C'CCAG_C Cuts at: 2762 4823 5319 2762 Size: 2061 496 3063 Fragments arranged by size: 3063 2061 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5047 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3070 3831 4859 1540 Size: 275 186 1069 761 1028 2301 Fragments arranged by size: 2301 1069 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2505 2548 3681 3766 4837 2505 Size: 43 1133 85 1071 3288 Fragments arranged by size: 3288 1133 1071 85 43 BsmI GAATG_Cn' Cuts at: 3131 3224 3131 Size: 93 5527 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2505 2548 2762 2939 3681 3766 Size: 399 424 291 43 214 177 742 85 Cuts at: 3766 4837 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 424 399 291 214 177 85 43 BspEI T'CCGG_A Cuts at: 5283 5283 Size: 5620 BspHI T'CATG_A Cuts at: 2382 2560 3509 5239 2382 Size: 178 949 1730 2763 Fragments arranged by size: 2763 1730 949 178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2705 2975 844 Size: 1861 270 3489 Fragments arranged by size: 3489 1861 270 BsrBI CCG'CTC Cuts at: 1394 1823 3507 1394 Size: 429 1684 3507 Fragments arranged by size: 3507 1684 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2289 2595 4115 4289 65 Size: 1183 1041 306 1520 174 1396 Fragments arranged by size: 1520 1396 1183 1041 306 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4264 4451 1700 Size: 110 2454 187 2869 Fragments arranged by size: 2869 2454 187 110 BsrGI T'GTAC_A Cuts at: 96 2808 96 Size: 2712 2908 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 3680 4692 3680 Size: 1012 4608 BstAPI GCAn_nnn'nTGC Cuts at: 794 2572 794 Size: 1778 3842 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5620 BstXI CCAn_nnnn'nTGG Cuts at: 2278 2278 Size: 5620 BstYI r'GATC_y Cuts at: 1972 2658 2950 3317 3702 3719 5171 5257 5269 Size: 686 292 367 385 17 1452 86 12 Cuts at: 5269 5615 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 686 385 367 346 292 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2852 2852 Size: 5620 BtgI C'CryG_G Cuts at: 513 1065 2004 2328 513 Size: 552 939 324 3805 Fragments arranged by size: 3805 939 552 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4881 Fragments arranged by size: 4881 683 56 BtsI GCAGTG_nn' Cuts at: 2196 2208 2308 2825 3136 3930 3950 2196 Size: 12 100 517 311 794 20 3866 Fragments arranged by size: 3866 794 517 311 100 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5620 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2924 2924 Size: 5620 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 2349 2674 Size: 54 1425 50 275 18 393 126 325 Cuts at: 2674 3067 3956 5585 8 Size: 393 889 1629 43 Fragments arranged by size: 1629 1425 889 393 393 325 275 126 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3067 1537 Size: 275 1255 4090 Fragments arranged by size: 4090 1255 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2707 3550 1353 Size: 1354 843 3423 Fragments arranged by size: 3423 1354 843 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4207 4581 4727 387 Size: 1064 138 2618 374 146 1280 Fragments arranged by size: 2618 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2752 3683 4280 5067 5335 1239 Size: 393 1120 931 597 787 268 1524 Fragments arranged by size: 1524 1120 931 787 597 393 268 EcoRI G'AATT_C Cuts at: 2929 3307 2929 Size: 378 5242 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 4767 1678 Size: 3089 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2239 2271 2377 2409 4261 Size: 32 135 32 667 32 106 32 1852 Cuts at: 4261 4293 4335 4367 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1852 667 135 106 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2964 3210 669 Size: 988 281 1026 246 3079 Fragments arranged by size: 3079 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5620 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2945 2945 Size: 5620 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4734 4918 1305 Size: 3429 184 2007 Fragments arranged by size: 3429 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2225 2351 10 Size: 54 1425 343 393 126 3279 Fragments arranged by size: 3279 1425 393 343 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 2150 2201 2276 2559 2600 2717 Size: 648 948 36 51 75 283 41 117 Cuts at: 2717 3578 3937 4096 518 Size: 861 359 159 2042 Fragments arranged by size: 2042 948 861 648 359 283 159 117 75 51 41 36 MspA1I CmG'CkG Cuts at: 1606 3036 3713 4861 5106 1606 Size: 1430 677 1148 245 2120 Fragments arranged by size: 2120 1430 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2328 513 Size: 552 939 324 3805 Fragments arranged by size: 3805 939 552 324 NdeI CA'TA_TG Cuts at: 387 387 Size: 5620 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NsiI A_TGCA'T Cuts at: 2535 2535 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2537 2978 4523 1132 Size: 97 1308 441 1545 2229 Fragments arranged by size: 2229 1545 1308 441 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 2221 1108 Size: 1113 4507 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3721 5202 5234 3689 Size: 32 1481 32 4075 Fragments arranged by size: 4075 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5620 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2871 2903 2871 Size: 32 5588 PstI C_TGCA'G Cuts at: 830 2203 2730 2972 830 Size: 1373 527 242 3478 Fragments arranged by size: 3478 1373 527 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2962 1936 Size: 1026 4594 SapI GCTCTTCn'nnn_ Cuts at: 2707 2707 Size: 5620 SbfI CC_TGCA'GG Cuts at: 2972 2972 Size: 5620 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2199 2234 2726 2968 4103 4784 4975 Size: 219 1154 35 492 242 1135 681 191 Cuts at: 4975 826 Size: 1471 Fragments arranged by size: 1471 1154 1135 681 492 242 219 191 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2947 1872 Size: 1075 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2654 3724 4625 4887 820 Size: 197 926 711 1070 901 262 1553 Fragments arranged by size: 1553 1070 926 901 711 262 197 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2978 1132 Size: 1846 3774 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5620 StyI C'CwwG_G Cuts at: 513 1065 2004 2062 2328 3059 513 Size: 552 939 58 266 731 3074 Fragments arranged by size: 3074 939 731 552 266 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2288 3647 3664 3817 4002 4447 325 Size: 1255 708 1359 17 153 185 445 1498 Fragments arranged by size: 1498 1359 1255 708 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2154 2808 3866 Size: 274 80 33 51 1443 177 654 1058 Cuts at: 3866 5578 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1058 654 274 177 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5580 5612 5580 Size: 32 5588 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2669 2257 Size: 412 5208 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5620 XmaI C'CCGG_G Cuts at: 1870 2945 1870 Size: 1075 4545 XmnI GAAnn'nnTTC Cuts at: 3749 3749 Size: 5620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI