(Circular) (Six-base) MAPSORT of: pFN21AE0864.seq Check: 9175 from: 1 to: 5662 pFN21AE0864 5662 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2747 2747 Size: 5662 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3472 278 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2850 3005 1937 Size: 913 155 4594 Fragments arranged by size: 4594 913 155 Acc65I G'GTAC_C Cuts at: 2983 2983 Size: 5662 AclI AA'CG_TT Cuts at: 1563 2961 3789 4162 1563 Size: 1398 828 373 3063 Fragments arranged by size: 3063 1398 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3725 5109 5377 1632 Size: 2093 1384 268 1917 Fragments arranged by size: 2093 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5465 AflIII A'CryG_T Cuts at: 1203 2673 4561 5538 1203 Size: 1470 1888 977 1327 Fragments arranged by size: 1888 1470 1327 977 AgeI A'CCGG_T Cuts at: 4493 4493 Size: 5662 AhdI GACnn_n'nnGTC Cuts at: 4391 4391 Size: 5662 AleI CACnn'nnGTG Cuts at: 1166 2240 1166 Size: 1074 4588 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2462 2651 1184 Size: 1278 189 4195 Fragments arranged by size: 4195 1278 189 AlwNI CAG_nnn'CTG Cuts at: 2078 2449 2951 3405 4977 2078 Size: 371 502 454 1572 2763 Fragments arranged by size: 2763 1572 502 454 371 ApaI G_GGCC'C Cuts at: 2281 2314 2461 2281 Size: 33 147 5482 Fragments arranged by size: 5482 147 33 ApaLI G'TGCA_C Cuts at: 2164 2437 2600 3719 4875 2164 Size: 273 163 1119 1156 2951 Fragments arranged by size: 2951 1156 1119 273 163 ApoI r'AATT_y Cuts at: 1493 2081 2971 3197 3349 5458 1493 Size: 588 890 226 152 2109 1697 Fragments arranged by size: 2109 1697 890 588 226 152 AseI AT'TA_AT Cuts at: 160 1032 4216 160 Size: 872 3184 1606 Fragments arranged by size: 3184 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 AvaI C'yCGr_G Cuts at: 1870 1943 2987 1870 Size: 73 1044 4545 Fragments arranged by size: 4545 1044 73 BamHI G'GATC_C Cuts at: 2992 3359 2992 Size: 367 5295 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2983 4438 618 Size: 325 217 627 1196 1455 1842 Fragments arranged by size: 1842 1455 1196 627 325 217 BanII G_rGCy'C Cuts at: 1391 2281 2314 2461 2981 1391 Size: 890 33 147 520 4072 Fragments arranged by size: 4072 890 520 147 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2017 928 Size: 971 118 4573 Fragments arranged by size: 4573 971 118 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3853 3887 1903 Size: 34 50 34 1832 34 3678 Fragments arranged by size: 3678 1832 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3554 4764 3554 Size: 1210 4452 BclI T'GATC_A Cuts at: 1548 1548 Size: 5662 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4273 136 Size: 107 122 71 631 605 2601 1525 Fragments arranged by size: 2601 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5657 5657 Size: 5662 BlpI GC'TnA_GC Cuts at: 3079 3079 Size: 5662 Bme1580I G_kGCm'C Cuts at: 1790 2168 2281 2314 2441 2461 2604 3723 4879 Size: 378 113 33 127 20 143 1119 1156 Cuts at: 4879 1790 Size: 2573 Fragments arranged by size: 2573 1156 1119 378 143 127 113 33 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5662 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3393 4351 442 Size: 416 53 83 399 24 1976 958 1753 Fragments arranged by size: 1976 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5662 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4322 1239 Size: 3083 2579 Bpu10I CC'TnA_GC Cuts at: 2696 2696 Size: 5662 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3787 4652 4950 3787 Size: 865 298 4499 Fragments arranged by size: 4499 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4325 882 Size: 617 2826 2219 Fragments arranged by size: 2826 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2674 3453 493 Size: 711 1470 779 2702 Fragments arranged by size: 2702 1470 779 711 BsaBI GATnn'nnATC Cuts at: 5656 5656 Size: 5662 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2955 3469 3851 4486 Size: 53 83 186 936 1422 514 382 635 Cuts at: 4486 275 Size: 1451 Fragments arranged by size: 1451 1422 936 635 514 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4095 4493 4767 4914 5325 4095 Size: 398 274 147 411 4432 Fragments arranged by size: 4432 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5437 Fragments arranged by size: 5437 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 2105 2938 1186 Size: 916 3 833 3910 Fragments arranged by size: 3910 916 833 3 BseYI C'CCAG_C Cuts at: 2449 4865 5361 2449 Size: 2416 496 2750 Fragments arranged by size: 2750 2416 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2390 1163 Size: 573 654 4435 Fragments arranged by size: 4435 654 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3112 3873 4901 1540 Size: 275 186 1111 761 1028 2301 Fragments arranged by size: 2301 1111 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2168 2441 2604 2920 3723 3808 4879 2168 Size: 273 163 316 803 85 1071 2951 Fragments arranged by size: 2951 1071 803 316 273 163 85 BsmI GAATG_Cn' Cuts at: 3173 3266 3173 Size: 93 5569 BsmBI CGTCTCn'nnnn_ Cuts at: 2671 2671 Size: 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2281 2314 2441 2461 2604 2920 Size: 399 378 113 33 127 20 143 316 Cuts at: 2920 2981 3723 3808 4879 1391 Size: 61 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 378 316 143 127 113 85 61 33 20 BspEI T'CCGG_A Cuts at: 5325 5325 Size: 5662 BspHI T'CATG_A Cuts at: 2421 3551 5281 2421 Size: 1130 1730 2802 Fragments arranged by size: 2802 1730 1130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2747 2805 3017 844 Size: 1903 58 212 3489 Fragments arranged by size: 3489 1903 212 58 BsrBI CCG'CTC Cuts at: 1394 1823 3549 1394 Size: 429 1726 3507 Fragments arranged by size: 3507 1726 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4157 4331 65 Size: 1183 2909 174 1396 Fragments arranged by size: 2909 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4306 4493 1700 Size: 110 2496 187 2869 Fragments arranged by size: 2869 2496 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5662 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5662 BssSI C'ACGA_G Cuts at: 2633 2789 3722 4734 2633 Size: 156 933 1012 3561 Fragments arranged by size: 3561 1012 933 156 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5662 BstBI TT'CG_AA Cuts at: 4466 4466 Size: 5662 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5662 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2272 2296 2011 Size: 261 24 5377 Fragments arranged by size: 5377 261 24 BstYI r'GATC_y Cuts at: 1972 2992 3359 3744 3761 5213 5299 5311 5657 Size: 1020 367 385 17 1452 86 12 346 Cuts at: 5657 1972 Size: 1977 Fragments arranged by size: 1977 1452 1020 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2894 2894 Size: 5662 BtgI C'CryG_G Cuts at: 513 1065 2241 2757 513 Size: 552 1176 516 3418 Fragments arranged by size: 3418 1176 552 516 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2571 487 Size: 683 56 1345 3578 Fragments arranged by size: 3578 1345 683 56 BtsI GCAGTG_nn' Cuts at: 3178 3972 3992 3178 Size: 794 20 4848 Fragments arranged by size: 4848 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5662 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2032 2067 529 Size: 35 1468 35 4124 Fragments arranged by size: 4124 1468 35 35 DraI TTT'AAA Cuts at: 2966 2966 Size: 5662 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4669 809 Size: 507 3353 1802 Fragments arranged by size: 3353 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2524 3109 3998 Size: 54 1425 50 275 18 694 585 889 Cuts at: 3998 5627 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 694 585 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3109 1537 Size: 275 1297 4090 Fragments arranged by size: 4090 1297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 2111 2480 3592 1353 Size: 749 9 369 1112 3423 Fragments arranged by size: 3423 1112 749 369 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4249 4623 4769 387 Size: 1064 138 2660 374 146 1280 Fragments arranged by size: 2660 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3725 4322 5109 5377 1239 Size: 393 2093 597 787 268 1524 Fragments arranged by size: 2093 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2277 2310 2457 2458 2277 Size: 33 147 1 5481 Fragments arranged by size: 5481 147 33 1 EcoRI G'AATT_C Cuts at: 2081 2971 3349 2081 Size: 890 378 4394 Fragments arranged by size: 4394 890 378 FspI TGC'GCA Cuts at: 4168 4168 Size: 5662 HaeII r_GCGC'y Cuts at: 1678 4809 1678 Size: 3131 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4303 4335 4377 4409 1373 Size: 32 135 32 2731 32 42 32 2626 Fragments arranged by size: 2731 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3006 3252 669 Size: 988 281 1068 246 3079 Fragments arranged by size: 3079 1068 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5662 HpaI GTT'AAC Cuts at: 3252 3252 Size: 5662 KpnI G_GTAC'C Cuts at: 2987 2987 Size: 5662 MfeI C'AATT_G Cuts at: 3261 3261 Size: 5662 MluI A'CGCG_T Cuts at: 5538 5538 Size: 5662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 2289 4776 4960 1305 Size: 927 57 2487 184 2007 Fragments arranged by size: 2487 2007 927 184 57 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3840 Fragments arranged by size: 3840 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2156 2189 2240 2444 2597 2633 Size: 648 843 147 33 51 204 153 36 Cuts at: 2633 3620 3979 4138 518 Size: 987 359 159 2042 Fragments arranged by size: 2042 987 843 648 359 204 159 153 147 51 36 33 MspA1I CmG'CkG Cuts at: 1606 2404 3078 3755 4903 5148 1606 Size: 798 674 677 1148 245 2120 Fragments arranged by size: 2120 1148 798 677 674 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5110 NdeI CA'TA_TG Cuts at: 387 387 Size: 5662 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5662 NotI GC'GGCC_GC Cuts at: 3109 3109 Size: 5662 NspI r_CATG'y Cuts at: 1132 1229 3020 4565 1132 Size: 97 1791 1545 2229 Fragments arranged by size: 2229 1791 1545 97 PasI CC'CwG_GG Cuts at: 1348 2461 1348 Size: 1113 4549 PciI A'CATG_T Cuts at: 4561 4561 Size: 5662 PflMI CCAn_nnn'nTGG Cuts at: 1108 2154 1108 Size: 1046 4616 PfoI T'CCnGG_A Cuts at: 2220 2220 Size: 5662 PmeI GTTT'AAAC Cuts at: 2966 2966 Size: 5662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2039 2071 2689 2721 3731 3763 5244 5276 2039 Size: 32 618 32 1010 32 1481 32 2425 Fragments arranged by size: 2425 1481 1010 618 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5662 PsiI TTA'TAA Cuts at: 3232 3232 Size: 5662 PspOMI G'GGCC_C Cuts at: 2277 2310 2457 2277 Size: 33 147 5482 Fragments arranged by size: 5482 147 33 PstI C_TGCA'G Cuts at: 830 2490 2772 2786 3014 830 Size: 1660 282 14 228 3478 Fragments arranged by size: 3478 1660 282 228 14 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5662 SalI G'TCGA_C Cuts at: 1936 3004 1936 Size: 1068 4594 SbfI CC_TGCA'GG Cuts at: 3014 3014 Size: 5662 ScaI AGT'ACT Cuts at: 3910 5622 3910 Size: 1712 3950 SfcI C'TryA_G Cuts at: 826 1045 2486 2768 2782 3010 4145 4826 5017 Size: 219 1441 282 14 228 1135 681 191 Cuts at: 5017 826 Size: 1471 Fragments arranged by size: 1471 1441 1135 681 282 228 219 191 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5662 SmaI CCC'GGG Cuts at: 1872 2989 1872 Size: 1117 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3766 4667 4929 820 Size: 197 926 1823 901 262 1553 Fragments arranged by size: 1823 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3453 493 Size: 2960 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5662 SphI G_CATG'C Cuts at: 1132 3020 1132 Size: 1888 3774 SspI AAT'ATT Cuts at: 5 52 3586 5 Size: 47 3534 2081 Fragments arranged by size: 3534 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5662 StyI C'CwwG_G Cuts at: 513 1065 3101 513 Size: 552 2036 3074 Fragments arranged by size: 3074 2036 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2146 2441 2796 3689 3706 3859 4044 Size: 1255 566 295 355 893 17 153 185 Cuts at: 4044 4489 325 Size: 445 1498 Fragments arranged by size: 1498 1255 893 566 445 355 295 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3908 5620 96 Size: 274 80 33 51 1443 1931 1712 138 Fragments arranged by size: 1931 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5622 5654 5622 Size: 32 5630 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5662 XbaI T'CTAG_A Cuts at: 2998 2998 Size: 5662 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2155 2155 Size: 5662 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5662 XmaI C'CCGG_G Cuts at: 1870 2987 1870 Size: 1117 4545 XmnI GAAnn'nnTTC Cuts at: 3791 3791 Size: 5662 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3470 276 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI