Restriction map for
pFN21AE0862
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AvaII AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HindIII HinfI HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AciI 657 CCGC CCGC(-3/-1)
AcsI 886 GAATTC [GA]^AATT[CT]
AflII 777 CTTAAG C^TTAAG
AhdI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 70 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 886 GAATTC [GA]^AATT[CT]
AspEI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 400 GTGCTC G[AT]GC[AT]^C
BanI 118 GGCACC G^G[CT][GA]CC
BanII 158 GAGCCC G[GA]GC[CT]^C
BbsI 267 GAAGAC GAAGAC(2/6)
BfrI 777 CTTAAG C^TTAAG
BpuAI 267 GAAGAC GAAGAC(2/6)
BsiHKAI 400 GTGCTC G[AT]GC[AT]^C
BspMI 251 ACCTGC ACCTGC(4/8)
BsrI 558 ACTGG ACTGG(1/-1)
BstAPI 69 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 471 GGTCACC G^GT[ACGT]ACC
BstPI 471 GGTCACC G^GT[ACGT]ACC
Cac8I 342 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 390 GCGC GCG^C
Cfr13I 782 GGCCC G^G[ACGT]CC
DpnII 361 GATC ^GATC
DsaI 350 CCATGG C^C[GA][CT]GG
Eam1105I 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 313 CTCTTC CTCTTC(1/4)
EcoO65I 471 GGTCACC G^GT[ACGT]ACC
EcoRI 886 GAATTC G^AATTC
HapII 575 CCGG C^CGG
HhaI 390 GCGC GCG^C
HinP1I 390 GCGC G^CGC
HincII 247 GTTGAC GT[CT]^[GA]AC
HindII 247 GTTGAC GT[CT]^[GA]AC
HpaII 575 CCGG C^CGG
Ksp632I 313 CTCTTC CTCTTC(1/4)
MboI 361 GATC ^GATC
MspI 575 CCGG C^CGG
NcoI 350 CCATGG C^CATGG
NdeII 361 GATC ^GATC
PstI 67 CTGCAG CTGCA^G
Sau3AI 361 GATC ^GATC
Sau96I 782 GGCCC G^G[ACGT]CC
SmlI 777 CTTAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 177 GTAC GT^AC
232 GTAC
BalI 59 TGGCCA TGG^CCA
371 TGGCCA
BfaI 484 CTAG C^TAG
643 CTAG
BsiYI 56 CCCTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
552 CCTTAAACTGG
BslI 56 CCCTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
552 CCTTAAACTGG
BsmAI 463 GTCTC GTCTC(1/5)
646 GTCTC
BstXI 143 CCATTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
236 CCAAGATGCTGG
EcoT14I 350 CCATGG C^C[AT][AT]GG
617 CCTTGG
HphI 24 GGTGA GGTGA(8/7)
473 TCACC
MaeI 484 CTAG C^TAG
643 CTAG
MluNI 59 TGGCCA TGG^CCA
371 TGGCCA
MscI 59 TGGCCA TGG^CCA
371 TGGCCA
MseI 554 TTAA T^TAA
778 TTAA
MspA1I 73 CAGCTG C[AC]G^C[GT]G
586 CAGCTG
NlaIV 118 GGCACC GG[ACGT]^[ACGT]CC
322 GGCTCC
PvuII 73 CAGCTG CAG^CTG
586 CAGCTG
RsaI 177 GTAC GT^AC
232 GTAC
SfcI 67 CTGCAG C^T[GA][CT]AG
753 CTACAG
StyI 350 CCATGG C^C[AT][AT]GG
617 CCTTGG
Tru9I 554 TTAA T^TAA
778 TTAA
Tsp45I 472 GTCAC ^GT[GC]AC
536 GTGAC
Tsp509I 793 AATT ^AATT
887 AATT
XcmI 140 CCACCATTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
272 CCATTTCTTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BbvI BmyI BsaJI BseRI
BsmFI Bsp1286I BssKI BstF5I BstNI
DdeI EaeI EcoRII Fnu4HI FokI
HaeIII ItaI MaeIII MboII MnlI
MslI MvaI MwoI NlaIII ScrFI
SfaNI TseI TspRI