Restriction map for
pFN21AE0851
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BbvI BcgI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FbaI Fnu4HI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII ItaI KasI KpnI
Ksp632I KspI MamI MboII MfeI
MflI MluI MluNI MroI MscI
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI TseI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 737 GTAC GT^AC
AlwI 84 GATCC GGATC(4/5)
AocI 846 CCTGAGG CC^T[ACGT]AGG
AseI 493 ATTAAT AT^TAAT
AsnI 493 ATTAAT AT^TAAT
AvaII 419 GGACC G^G[AT]CC
BanI 597 GGTGCC G^G[CT][GA]CC
BciVI 366 GTATCC GTATCC(6/5)
BpmI 136 CTCCAG CTGGAG(16/14)
BsaAI 340 TACGTG [CT]AC^GT[GA]
BsrBI 131 CCGCTC CCGCTC(-3/-3)
BsrDI 95 GCAATG GCAATG(2/0)
BsrI 44 ACTGG ACTGG(1/-1)
BssKI 416 CCTGG ^CC[ACGT]GG
BstNI 416 CCTGG CC^[AT]GG
Bsu36I 846 CCTGAGG CC^T[ACGT]AGG
Cac8I 531 GCTTGC GC[ACGT]^[ACGT]GC
Cfr13I 419 GGACC G^G[ACGT]CC
DpnII 84 GATC ^GATC
DraIII 300 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 846 CCTGAGG CC^T[ACGT]AGG
EcoRII 416 CCTGG ^CC[AT]GG
EcoRV 859 GATATC GAT^ATC
HaeIII 327 GGCC GG^CC
HindIII 529 AAGCTT A^AGCTT
MaeII 341 ACGT A^CGT
MboI 84 GATC ^GATC
MspA1I 400 CAGCTG C[AC]G^C[GT]G
MvaI 416 CCTGG CC^[AT]GG
NdeII 84 GATC ^GATC
NlaIV 597 GGTGCC GG[ACGT]^[ACGT]CC
NspI 451 ACATGC [GA]CATG^[CT]
PshBI 493 ATTAAT AT^TAAT
PvuII 400 CAGCTG CAG^CTG
RsaI 737 GTAC GT^AC
Sau3AI 84 GATC ^GATC
Sau96I 419 GGACC G^G[ACGT]CC
ScrFI 416 CCTGG CC^[ACGT]GG
SfcI 434 CTGTAG C^T[GA][CT]AG
SmlI 526 CTCAAG C^T[CT][GA]AG
SspI 244 AATATT AAT^ATT
Tsp45I 509 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 19 enzymes) #
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Asp700I 4 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
242 GAAATATTTC
BfaI 373 CTAG C^TAG
886 CTAG
BsiYI 336 CCGCTACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
472 CCCTTTTGCGG
BslI 336 CCGCTACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
472 CCCTTTTGCGG
BsmAI 308 GTCTC GTCTC(1/5)
619 GTCTC
BsmI 212 GAATGC GAATGC(1/-1)
395 GCATTC
BspMI 691 ACCTGC ACCTGC(4/8)
733 GCAGGT
FauI 130 CCCGC CCCGC(4/6)
516 CCCGC
HphI 126 TCACC GGTGA(8/7)
702 TCACC
MaeI 373 CTAG C^TAG
886 CTAG
MaeIII 193 GTAAC ^GT[ACGT]AC
509 GTGAC
MseI 494 TTAA T^TAA
898 TTAA
MslI 269 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
546 CATATTTGTG
PleI 141 GACTC GAGTC(4/5)
231 GACTC
SfaNI 48 GATGC GCATC(5/9)
101 GCATC
TfiI 816 GATTC G^A[AT]TC
828 GAATC
Tru9I 494 TTAA T^TAA
898 TTAA
TspRI 43 CACTG CA[GC]TG[ACGT][ACGT]^
320 CAGTG
XmnI 4 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
242 GAAATATTTC
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
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AciI AcsI AluI ApoI BstF5I
DdeI FokI HinfI MnlI NlaIII
Tsp509I