(Circular) (Six-base) MAPSORT of: pFN21AE0830.seq Check: 2890 from: 1 to: 5464 pFN21AE0830 5464 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2226 2226 Size: 5464 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2807 1937 Size: 870 4594 Acc65I G'GTAC_C Cuts at: 2785 2785 Size: 5464 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2405 3527 4911 5179 1632 Size: 773 1122 1384 268 1917 Fragments arranged by size: 1917 1384 1122 773 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 4363 5340 1203 Size: 3160 977 1327 Fragments arranged by size: 3160 1327 977 AgeI A'CCGG_T Cuts at: 4295 4295 Size: 5464 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5464 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2234 1184 Size: 1050 4414 AlwNI CAG_nnn'CTG Cuts at: 3207 4779 3207 Size: 1572 3892 ApaLI G'TGCA_C Cuts at: 3521 4677 3521 Size: 1156 4308 ApoI r'AATT_y Cuts at: 1493 2773 2999 3151 5260 1493 Size: 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 226 152 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2789 1870 Size: 73 846 4545 Fragments arranged by size: 4545 846 73 BamHI G'GATC_C Cuts at: 2794 3161 2794 Size: 367 5097 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2785 4240 618 Size: 325 217 627 998 1455 1842 Fragments arranged by size: 1842 1455 998 627 325 217 BanII G_rGCy'C Cuts at: 1391 2783 1391 Size: 1392 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2672 928 Size: 971 773 3720 Fragments arranged by size: 3720 971 773 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3655 3689 1903 Size: 34 1718 34 3678 Fragments arranged by size: 3678 1718 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2385 3356 4566 2385 Size: 971 1210 3283 Fragments arranged by size: 3283 1210 971 BclI T'GATC_A Cuts at: 1548 1548 Size: 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4075 136 Size: 107 122 71 631 605 2403 1525 Fragments arranged by size: 2403 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2038 2163 5459 2038 Size: 125 3296 2043 Fragments arranged by size: 3296 2043 125 BlpI GC'TnA_GC Cuts at: 2881 2881 Size: 5464 Bme1580I G_kGCm'C Cuts at: 1790 3525 4681 1790 Size: 1735 1156 2573 Fragments arranged by size: 2573 1735 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2289 3195 4153 Size: 416 53 83 399 24 872 906 958 Cuts at: 4153 442 Size: 1753 Fragments arranged by size: 1753 958 906 872 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4124 1239 Size: 2885 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3589 4454 4752 3589 Size: 865 298 4301 Fragments arranged by size: 4301 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4127 882 Size: 617 2628 2219 Fragments arranged by size: 2628 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3255 493 Size: 711 2051 2702 Fragments arranged by size: 2702 2051 711 BsaBI GATnn'nnATC Cuts at: 5458 5458 Size: 5464 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3271 3653 4288 275 Size: 53 83 186 936 1738 382 635 1451 Fragments arranged by size: 1738 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3897 4295 4569 4716 5127 3897 Size: 398 274 147 411 4234 Fragments arranged by size: 4234 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5239 Fragments arranged by size: 5239 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5464 BseYI C'CCAG_C Cuts at: 4667 5163 4667 Size: 496 4968 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 1163 Size: 573 468 4423 Fragments arranged by size: 4423 573 468 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3675 4703 1540 Size: 275 186 913 761 1028 2301 Fragments arranged by size: 2301 1028 913 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3525 3610 4681 3525 Size: 85 1071 4308 Fragments arranged by size: 4308 1071 85 BsmI GAATG_Cn' Cuts at: 2975 3068 2975 Size: 93 5371 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2783 3525 3610 4681 1391 Size: 399 993 742 85 1071 2174 Fragments arranged by size: 2174 1071 993 742 399 85 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 3353 5083 3353 Size: 1730 3734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2226 2819 844 Size: 1382 593 3489 Fragments arranged by size: 3489 1382 593 BsrBI CCG'CTC Cuts at: 1394 1823 3351 1394 Size: 429 1528 3507 Fragments arranged by size: 3507 1528 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2187 2661 3959 4133 65 Size: 1183 939 474 1298 174 1396 Fragments arranged by size: 1396 1298 1183 939 474 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4108 4295 1700 Size: 110 2298 187 2869 Fragments arranged by size: 2869 2298 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5464 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5464 BssSI C'ACGA_G Cuts at: 3524 4536 3524 Size: 1012 4452 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5464 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5464 BstYI r'GATC_y Cuts at: 1972 2038 2163 2794 3161 3546 3563 5015 5101 Size: 66 125 631 367 385 17 1452 86 Cuts at: 5101 5113 5459 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 631 385 367 346 125 86 66 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4912 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4725 Fragments arranged by size: 4725 683 56 BtsI GCAGTG_nn' Cuts at: 2980 3774 3794 2980 Size: 794 20 4650 Fragments arranged by size: 4650 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4471 809 Size: 507 3155 1802 Fragments arranged by size: 3155 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2911 3800 5429 Size: 54 1425 50 275 18 1081 889 1629 Cuts at: 5429 8 Size: 43 Fragments arranged by size: 1629 1425 1081 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2911 1537 Size: 275 1099 4090 Fragments arranged by size: 4090 1099 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2600 2654 3394 1353 Size: 1247 54 740 3423 Fragments arranged by size: 3423 1247 740 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4051 4425 4571 387 Size: 1064 138 2462 374 146 1280 Fragments arranged by size: 2462 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2405 3527 4124 4911 5179 1239 Size: 393 773 1122 597 787 268 1524 Fragments arranged by size: 1524 1122 787 773 597 393 268 EcoRI G'AATT_C Cuts at: 2773 3151 2773 Size: 378 5086 FspI TGC'GCA Cuts at: 3970 3970 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 4611 1678 Size: 2933 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4105 4137 4179 4211 1373 Size: 32 135 32 2533 32 42 32 2626 Fragments arranged by size: 2626 2533 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2808 3054 669 Size: 988 281 870 246 3079 Fragments arranged by size: 3079 988 870 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5464 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KpnI G_GTAC'C Cuts at: 2789 2789 Size: 5464 MfeI C'AATT_G Cuts at: 2744 3063 2744 Size: 319 5145 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4578 4762 1305 Size: 3273 184 2007 Fragments arranged by size: 3273 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3642 Fragments arranged by size: 3642 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3422 3781 3940 518 Size: 648 2256 359 159 2042 Fragments arranged by size: 2256 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2880 3557 4705 4950 1606 Size: 1274 677 1148 245 2120 Fragments arranged by size: 2120 1274 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4912 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2695 2822 4367 1132 Size: 97 1466 127 1545 2229 Fragments arranged by size: 2229 1545 1466 127 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5464 PciI A'CATG_T Cuts at: 4363 4363 Size: 5464 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5464 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5464 PsiI TTA'TAA Cuts at: 3034 3034 Size: 5464 PstI C_TGCA'G Cuts at: 830 2816 830 Size: 1986 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SbfI CC_TGCA'GG Cuts at: 2816 2816 Size: 5464 ScaI AGT'ACT Cuts at: 2360 3712 5424 2360 Size: 1352 1712 2400 Fragments arranged by size: 2400 1712 1352 SfcI C'TryA_G Cuts at: 826 1045 2548 2812 3947 4628 4819 826 Size: 219 1503 264 1135 681 191 1471 Fragments arranged by size: 1503 1471 1135 681 264 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5464 SmaI CCC'GGG Cuts at: 1872 2791 1872 Size: 919 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3568 4469 4731 820 Size: 197 926 1625 901 262 1553 Fragments arranged by size: 1625 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5464 SphI G_CATG'C Cuts at: 1132 2822 1132 Size: 1690 3774 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 2442 1517 Size: 925 4539 StyI C'CwwG_G Cuts at: 513 1065 2149 2903 513 Size: 552 1084 754 3074 Fragments arranged by size: 3074 1084 754 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3491 3508 3661 3846 4291 325 Size: 1255 1911 17 153 185 445 1498 Fragments arranged by size: 1911 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2276 2358 3710 Size: 274 80 33 51 1443 299 82 1352 Cuts at: 3710 5422 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1352 299 274 138 82 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5424 5456 5424 Size: 32 5432 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5464 XbaI T'CTAG_A Cuts at: 2800 2800 Size: 5464 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5464 XmaI C'CCGG_G Cuts at: 1870 2789 1870 Size: 919 4545 XmnI GAAnn'nnTTC Cuts at: 3593 3593 Size: 5464 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI