Restriction map for
pFN21AE0823
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanII BbeI BbvCI
BcgI BciVI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KspI MamI
MfeI MflI MluI MroI MseI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 415 GGTACC G^GTACC
AciI 363 CCGC CCGC(-3/-1)
AluI 557 AGCT AG^CT
Alw44I 483 GTGCAC G^TGCAC
AlwNI 238 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 483 GTGCAC G^TGCAC
Asp718I 415 GGTACC G^GTACC
AspHI 483 GTGCAC G[AT]GC[AT]^C
BanI 415 GGTACC G^G[CT][GA]CC
BbrPI 480 CACGTG CAC^GTG
BbsI 46 GTCTTC GAAGAC(2/6)
BclI 134 TGATCA T^GATCA
BglI 247 GCCACACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 483 GTGCAC G[AGT]GC[ACT]^C
BpmI 410 CTCCAG CTGGAG(16/14)
BpuAI 46 GTCTTC GAAGAC(2/6)
BsaAI 480 CACGTG [CT]AC^GT[GA]
BsiHKAI 483 GTGCAC G[AT]GC[AT]^C
Bsp1286I 483 GTGCAC G[AGT]GC[ACT]^C
BstXI 626 CCAATGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 464 GGGCC G^G[ACGT]CC
DraIII 327 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 79 CTCTTC CTCTTC(1/4)
EcoT22I 612 ATGCAT ATGCA^T
FbaI 134 TGATCA T^GATCA
KpnI 415 GGTACC GGTAC^C
Ksp632I 79 CTCTTC CTCTTC(1/4)
MaeII 481 ACGT A^CGT
NdeI 315 CATATG CA^TATG
NsiI 612 ATGCAT ATGCA^T
PleI 408 GACTC GAGTC(4/5)
PmaCI 480 CACGTG CAC^GTG
PmlI 480 CACGTG CAC^GTG
Sau96I 464 GGGCC G^G[ACGT]CC
SbfI 419 CCTGCAGG CCTGCA^GG
SfiI 246 GGCCACACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 419 CCTGCAGG CCTGCA^GG
TfiI 240 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 177 GTAC GT^AC
416 GTAC
BfaI 85 CTAG C^TAG
622 CTAG
BsiYI 60 CCCTGAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
671 CCATTTTGAGG
BslI 60 CCCTGAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
671 CCATTTTGAGG
BssKI 204 CCTGG ^CC[ACGT]GG
412 CCAGG
BstNI 204 CCTGG CC^[AT]GG
412 CCAGG
EcoRII 204 CCTGG ^CC[AT]GG
412 CCAGG
HinfI 240 GATTC G^A[ACGT]TC
408 GACTC
MaeI 85 CTAG C^TAG
622 CTAG
MslI 168 CACCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
399 CATGACCATG
MvaI 204 CCTGG CC^[AT]GG
412 CCAGG
MwoI 211 GCCTGCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
247 GCCACACTGGC
NlaIV 415 GGTACC GG[ACGT]^[ACGT]CC
463 GGGGCC
NspI 614 GCATGT [GA]CATG^[CT]
653 ACATGC
PstI 420 CTGCAG CTGCA^G
564 CTGCAG
RsaI 177 GTAC GT^AC
416 GTAC
ScrFI 204 CCTGG CC^[ACGT]GG
412 CCAGG
SfaNI 154 GATGC GCATC(5/9)
208 GATGC
SfcI 420 CTGCAG C^T[GA][CT]AG
564 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
BalI BbvI BsgI BspMI BsrI
BstF5I Cac8I DdeI DpnII EaeI
Fnu4HI FokI HaeIII HphI ItaI
MaeIII MboI MboII MluNI MnlI
MscI NdeII NlaIII Sau3AI TseI
Tsp45I TspRI