Restriction map for
pFN21AE0820
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# NO CUT ENZYMES ( 185 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaBI BsaI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MfeI MflI MluI MroI
MslI MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 163 GACGTC GACGT^C
AcsI 447 GAATTC [GA]^AATT[CT]
AcyI 163 GACGTC G[GA]^CG[CT]C
AfaI 423 GTAC GT^AC
ApoI 447 GAATTC [GA]^AATT[CT]
AvaII 275 GGTCC G^G[AT]CC
BalI 20 TGGCCA TGG^CCA
BbsI 336 GTCTTC GAAGAC(2/6)
BfaI 565 CTAG C^TAG
BpuAI 336 GTCTTC GAAGAC(2/6)
BsaAI 16 TACGTG [CT]AC^GT[GA]
BsaHI 163 GACGTC G[GA]^CG[CT]C
BsaJI 341 CCGAGG C^C[ACGT][ACGT]GG
BsgI 351 GTGCAG GTGCAG(16/14)
BsiEI 9 CGACCG CG[GA][CT]^CG
BsmAI 472 GTCTC GTCTC(1/5)
BsmFI 276 GTCCC GGGAC(10/14)
BspHI 245 TCATGA T^CATGA
BspMI 425 ACCTGC ACCTGC(4/8)
BssKI 201 CCTGG ^CC[ACGT]GG
BstNI 201 CCTGG CC^[AT]GG
DdeI 526 CTGAG C^T[ACGT]AG
DpnII 249 GATC ^GATC
DraII 274 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 20 TGGCCA [CT]^GGCC[GA]
EcoO109I 274 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 447 GAATTC G^AATTC
EcoRII 201 CCTGG ^CC[AT]GG
HgaI 57 GACGC GACGC(5/10)
Hin1I 163 GACGTC G[GA]^CG[CT]C
HphI 392 TCACC GGTGA(8/7)
MaeI 565 CTAG C^TAG
MaeIII 391 GTCAC ^GT[ACGT]AC
MboI 249 GATC ^GATC
MluNI 20 TGGCCA TGG^CCA
MscI 20 TGGCCA TGG^CCA
MseI 506 TTAA T^TAA
MvaI 201 CCTGG CC^[AT]GG
NdeII 249 GATC ^GATC
PpuMI 274 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 142 CAGCTG CAG^CTG
RcaI 245 TCATGA T^CATGA
RsaI 423 GTAC GT^AC
Sau3AI 249 GATC ^GATC
ScrFI 201 CCTGG CC^[ACGT]GG
Tru9I 506 TTAA T^TAA
Tsp45I 391 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 10 enzymes) #
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BsrI 271 ACTGG ACTGG(1/-1)
333 CCAGT
Cfr13I 204 GGCCC G^G[ACGT]CC
275 GGTCC
FauI 63 GCGGG CCCGC(4/6)
442 GCGGG
HaeIII 21 GGCC GG^CC
204 GGCC
MspA1I 61 CAGCGG C[AC]G^C[GT]G
142 CAGCTG
NlaIV 274 GGGTCC GG[ACGT]^[ACGT]CC
275 GGTCCC
Sau96I 204 GGCCC G^G[ACGT]CC
275 GGTCC
SfaNI 547 GATGC GCATC(5/9)
308 GCATC
Tsp509I 448 AATT ^AATT
495 AATT
TspRI 270 CACTG CA[GC]TG[ACGT][ACGT]^
384 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BstF5I Fnu4HI FokI ItaI MaeII
MboII MnlI NlaIII PstI SfcI
TseI