Restriction map for
pFN21AE0817
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# NO CUT ENZYMES ( 182 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HhaI HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MluI MluNI MroI MscI MseI
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 217 GACGTC GACGT^C
AflIII 413 ACATGT A^C[GA][CT]GT
AlwI 690 GATCC GGATC(4/5)
AvaII 63 GGTCC G^G[AT]CC
BciVI 673 GTATCC GTATCC(6/5)
BfaI 196 CTAG C^TAG
BpmI 696 CTCCAG CTGGAG(16/14)
BseRI 85 CTCCTC GAGGAG(10/8)
BsgI 429 CTGCAC GTGCAG(16/14)
BsiYI 698 CCAGTGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 698 CCAGTGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 219 CGTCTC CGTCTC(1/5)
BspLU11I 413 ACATGT A^CATGT
BspMI 292 GCAGGT ACCTGC(4/8)
BsrDI 528 CATTGC GCAATG(2/0)
BssKI 351 CCTGG ^CC[ACGT]GG
BstF5I 477 CATCC GGATG(2/0)
BstNI 351 CCTGG CC^[AT]GG
BstYI 689 AGATCC [GA]^GATC[CT]
EcoRII 351 CCTGG ^CC[AT]GG
FokI 477 CATCC GGATG(9/13)
HgaI 422 GACGC GACGC(5/10)
HphI 4 GGTGA GGTGA(8/7)
MaeI 196 CTAG C^TAG
MaeII 218 ACGT A^CGT
MfeI 638 CAATTG C^AATTG
MflI 689 AGATCC [GA]^GATC[CT]
MspA1I 169 CAGCTG C[AC]G^C[GT]G
MunI 638 CAATTG C^AATTG
MvaI 351 CCTGG CC^[AT]GG
NlaIV 62 GGGTCC GG[ACGT]^[ACGT]CC
NspI 413 ACATGT [GA]CATG^[CT]
PleI 524 GAGTC GAGTC(4/5)
PvuII 169 CAGCTG CAG^CTG
ScrFI 351 CCTGG CC^[ACGT]GG
SfcI 111 CTATAG C^T[GA][CT]AG
Tsp45I 5 GTGAC ^GT[GC]AC
XhoII 689 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 66 CCGC CCGC(-3/-1)
372 CCGC
AcyI 217 GACGTC G[GA]^CG[CT]C
422 GACGCC
AluI 170 AGCT AG^CT
733 AGCT
BbvI 355 GCAGC GCAGC(8/12)
731 GCAGC
BsaBI 144 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
547 GATTCTTATC
BsaHI 217 GACGTC G[GA]^CG[CT]C
422 GACGCC
BsaJI 251 CCATGG C^C[ACGT][ACGT]GG
359 CCATGG
BsmAI 220 GTCTC GTCTC(1/5)
283 GTCTC
DpnII 108 GATC ^GATC
690 GATC
DsaI 251 CCATGG C^C[GA][CT]GG
359 CCATGG
EarI 88 CTCTTC CTCTTC(1/4)
395 CTCTTC
EcoT14I 251 CCATGG C^C[AT][AT]GG
359 CCATGG
Fnu4HI 355 GCAGC GC^[ACGT]GC
731 GCAGC
Hin1I 217 GACGTC G[GA]^CG[CT]C
422 GACGCC
ItaI 355 GCAGC GC^[ACGT]GC
731 GCAGC
Ksp632I 88 CTCTTC CTCTTC(1/4)
395 CTCTTC
MamI 144 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
547 GATTCTTATC
MboI 108 GATC ^GATC
690 GATC
MwoI 241 GCTCCTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
355 GCAGCCATGGC
NcoI 251 CCATGG C^CATGG
359 CCATGG
NdeII 108 GATC ^GATC
690 GATC
Sau3AI 108 GATC ^GATC
690 GATC
StyI 251 CCATGG C^C[AT][AT]GG
359 CCATGG
TfiI 163 GATTC G^A[AT]TC
547 GATTC
TseI 355 GCAGC G^C[AT]GC
731 GCAGC
Tsp509I 558 AATT ^AATT
639 AATT
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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BsrI Cfr13I DdeI HaeIII HinfI
MaeIII MboII MnlI MslI NlaIII
Sau96I TspRI